HADH Antibody - #DF6221

(2)  
产品: HADH 抗体
货号: DF6221
描述: Rabbit polyclonal antibody to HADH
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 34kDa; 34kD(Calculated).
蛋白号: Q16836
RRID: AB_2838187

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MS Report
HPLC Report
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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Zebrafish(100%), Bovine(89%), Horse(100%), Sheep(89%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
HADH Antibody detects endogenous levels of total HADH.
RRID:
AB_2838187
引用格式: Affinity Biosciences Cat# DF6221, RRID:AB_2838187.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

3 hydroxyacyl Coenzyme A dehydrogenase; HAD; HADH; HADH1; HADHSC; HADHSC, formerly; HADSC, formerly; HCDH; HCDH_HUMAN; HHF4; Hydroxyacyl CoA dehydrogenase; Hydroxyacyl-coenzyme A dehydrogenase; hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; L 3 hydroxyacyl Coenzyme A dehydrogenase short chain; M SCHAD; Medium and short chain L 3 hydroxyacyl coenzyme A dehydrogenase; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; MGC8392; mitochondrial; MSCHAD; OTTHUMP00000162626; OTTHUMP00000219688; SCHAD; SCHAD, formerly; Short chain 3 hydroxyacyl CoA dehydrogenase mitochondrial; short chain 3-hydroxyacyl-coa dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase;

抗原和靶标

免疫原:
Uniprot:

Q16836(HCDH_HUMAN) >>Visit HPA database.

基因/基因ID:
HADH(3033)
表达:
Q16836 HCDH_HUMAN:

Expressed in liver, kidney, pancreas, heart and skeletal muscle.

描述:
This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15. [provided by RefSeq, May 2010]
序列:
MAFVTRQFMRSVSSSSTASASAKKIIVKHVTVIGGGLMGAGIAQVAAATGHTVVLVDQTEDILAKSKKGIEESLRKVAKKKFAENLKAGDEFVEKTLSTIATSTDAASVVHSTDLVVEAIVENLKVKNELFKRLDKFAAEHTIFASNTSSLQITSIANATTRQDRFAGLHFFNPVPVMKLVEVIKTPMTSQKTFESLVDFSKALGKHPVSCKDTPGFIVNRLLVPYLMEAIRLYERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIVDGWHEMDAENPLHQPSPSLNKLVAENKFGKKTGEGFYKYK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Bovine
89
Sheep
89
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q16836 作为底物

Site PTM Type Enzyme
S13 Phosphorylation
K68 Ubiquitination
S73 Phosphorylation
R75 Methylation
K76 Acetylation
K80 Acetylation
K81 Acetylation
K87 Acetylation
K87 Ubiquitination
K127 Ubiquitination
K132 Acetylation
K179 Acetylation
K185 Acetylation
K185 Ubiquitination
K192 Acetylation
K192 Ubiquitination
T193 Phosphorylation
S196 Phosphorylation
S201 Phosphorylation
K202 Acetylation
K202 Ubiquitination
K206 Ubiquitination
K212 Acetylation
K212 Ubiquitination
T214 Phosphorylation
Y226 Phosphorylation
Y234 Phosphorylation
K241 Acetylation
K241 Ubiquitination
K249 Acetylation
Y264 Phosphorylation
T269 Phosphorylation
T270 Phosphorylation
S290 Phosphorylation
K301 Ubiquitination
K304 Acetylation
K312 Acetylation
K312 Methylation
K312 Ubiquitination
Y313 Phosphorylation

研究背景

功能:

Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA.

翻译修饰:

Succinylation at Lys-81, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5 (By similarity).

细胞定位:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in liver, kidney, pancreas, heart and skeletal muscle.

亚基结构:

Homodimer.

蛋白家族:

Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

研究领域

· Metabolism > Lipid metabolism > Fatty acid elongation.

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Amino acid metabolism > Lysine degradation.

· Metabolism > Amino acid metabolism > Tryptophan metabolism.

· Metabolism > Carbohydrate metabolism > Butanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

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