PSMD4 Antibody - #DF6206

(7)  
产品: PSMD4 抗体
货号: DF6206
描述: Rabbit polyclonal antibody to PSMD4
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 41kDa, 52kDa; 41kD(Calculated).
蛋白号: P55036
RRID: AB_2838172

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
PSMD4 Antibody detects endogenous levels of total PSMD4.
RRID:
AB_2838172
引用格式: Affinity Biosciences Cat# DF6206, RRID:AB_2838172.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

26S protease subunit S5a; 26S proteasome non ATPase regulatory subunit 4; 26S proteasome non-ATPase regulatory subunit 4; 26S proteasome regulatory subunit rpn10; 26S proteasome regulatory subunit S5A; AF 1; AF; AF1; Angiocidin; Antisecretory factor 1; ASF; DS5a; MCB 1; MCB1; Multiubiquitin chain binding protein; Multiubiquitin chain-binding protein; OTTHUMP00000059963; Prosome macropain; Proteasome (prosome macropain) 26S subunit non ATPase 4; Proteasome 26S non ATPase subunit 4; Proteasome 26S subunit non ATPase 4; PSMD 4; Psmd4; PSMD4_HUMAN; pUB R5; pUBR5; Rpn 10; Rpn10; RPN10 homolog; S5A; S5a/antisecretory factor protein;

抗原和靶标

免疫原:
Uniprot:

P55036(PSMD4_HUMAN) >>Visit HPA database.

基因/基因ID:
PSMD4(5710)
描述:
S5a (PSMD4) is a subunit of the 19S regulatory proteasome complex functioning in ubiquitinated-protein targeting and degradation (1). S5a contains two polyubiquitin binding motifs (UIM) that bind multiubiquitin chains by hydrophobic interaction (2,3). In addition to ubiquitin, the UIM of S5a shows high affinity to a ubiquitin-like domain present in many proteins. S5a binds to these types of proteins directly and mediates their targeting to the proteasome for degradation (4,5).
序列:
MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATTGTEDSDDALLKMTISQQEFGRTGLPDLSSMTEEEQIAYAMQMSLQGAEFGQAESADIDASSAMDTSEPAKEEDDYDVMQDPEFLQSVLENLPGVDPNNEAIRNAMGSLASQATKDGKKDKKEEDKK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P55036 作为底物

Site PTM Type Enzyme
C9 S-Nitrosylation
C37 S-Nitrosylation
K40 Ubiquitination
K74 Ubiquitination
K81 Ubiquitination
K83 Ubiquitination
C87 S-Nitrosylation
K98 Ubiquitination
S115 Phosphorylation
K122 Acetylation
K122 Ubiquitination
K126 Acetylation
K126 Ubiquitination
K129 Ubiquitination
K133 Ubiquitination
K135 Ubiquitination
K152 Ubiquitination
S242 Phosphorylation
T250 Phosphorylation
T253 Phosphorylation
S256 Phosphorylation
K262 Ubiquitination
S266 Phosphorylation
Y326 Phosphorylation
S358 Phosphorylation
S361 Phosphorylation
K365 Acetylation
K365 Ubiquitination
K368 Acetylation
K369 Ubiquitination

研究背景

功能:

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.

亚基结构:

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases and few additional components including PSMD4. Interacts with NUB1. Interacts with SQSTM1. Interacts with UBQLN4. Interacts with UBE3A. Interacts with UBQLN1 (via ubiquitin-like domain). Interacts with DDI2.

蛋白家族:

The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.

Belongs to the proteasome subunit S5A family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.