Synaptotagmin 1 Antibody - #DF6174

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产品: Synaptotagmin 1 抗体
货号: DF6174
描述: Rabbit polyclonal antibody to Synaptotagmin 1
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: P21579
RRID: AB_2838141

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Synaptotagmin 1 Antibody detects endogenous levels of total Synaptotagmin 1.
RRID:
AB_2838141
引用格式: Affinity Biosciences Cat# DF6174, RRID:AB_2838141.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZp781D2042; FLJ42519; p65; SVP65; Synaptotagmin 2; Synaptotagmin I; Synaptotagmin II; Synaptotagmin-1; SYT; Syt1; SYT1_HUMAN; Syt2; SytI;

抗原和靶标

免疫原:

A synthesized peptide derived from human Synaptotagmin 1, corresponding to a region within the internal amino acids.

基因/基因ID:
SYT1(6857)
描述:
Synaptotagmin 1 (SYT1) is an integral membrane protein found in synaptic vesicles thought to play a role in vesicle trafficking and exocytosis (1). Individual SYT1 proteins are composed of an amino-terminal transmembrane region, a central linker region and a pair of carboxy-terminal C2 domains responsible for binding Ca2+ (2). The C2 domains appear to be functionally distinct, with the C2A domain responsible for regulating synaptic vesicle fusion in a calcium-dependent manner during exocytosis while the C2B domain allows for interaction between adjacent SYT1 proteins (3). Because synaptotagmin 1 binds calcium and is found in synaptic vesicles, this integral membrane protein is thought act as a calcium sensor in fast synaptic vesicle exocytosis. Evidence suggests possible roles in vesicle-mediated endocytosis and glucose-induced insulin secretion as well (4,5). SYT1 binds several different SNARE proteins during calcium-mediated vesicle endocytosis and an association between SYT1 and the SNARE protein SNAP-25 is thought to be a key element in vesicle-mediated exocytosis (6).

研究领域

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

文献引用

1). Electroacupuncture may alleviate neuropathic pain via suppressing P2X7R expression. Molecular Pain, 2021 (PubMed: 33626989) [IF=3.3]

Application: WB    Species: rats    Sample: spinal dorsal horn

Figure 2. EA reduced abnormal remodeling of dendritic spines/synapses, whereas BzATP had the opposite effects. (a) and (b) Representative Western blots and quantification data of the expression of Synaptotagmin 1/GAPDH. (n ¼ 3). (c) Dendritic spines. Scale bars, 10 lm. (d) Number of spines per 10 lm. (n ¼ 3). (e) The ultrastructure of synapses in the spinal dorsal horn from rats in each group (0.2 lm, 30,000 ). (f) Number of synapses per 30000x field. (n ¼ 4). (g) Width of synaptic space (nm). (n ¼ 4). (h) Vesicle density (vesicles/ mm2 ). (n ¼ 4). Columns represent the mean  SD. #p < 0.05 and ##p < 0.01 vs. the S group; $p < 0.05 and $$p < 0.01 vs. the M group; &&p < 0.01 vs. the MþEA group.

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