SIRT7 Antibody - #DF6161

FIGURE 2 | Effects of the RvE1 and LXA4 combination on NF-κB modification.(F) Phosphorylation and acetylation levels of p65 and expressions of SIRT1, SIRT6 and SIRT7 protein (normalized to that of β-tubulin) detected by western blotting.

Figure 1 miR-335-5p and SIRT7 expression following treatment with high glucose, TNF-α, and H2O2 (A) Relative miR-335-5p expression in HUVECs determined using RT-PCR assay (n=3, in all groups). (B) Relative SIRT7 expression in HUVECs determined using RT-PCR assay (n=3, in all groups). (C) Western blot assay on HUVECs for SIRT7 expression following SIRT7–siRNA transfection (n=3, in all groups). NS: non-significance, **P<0.01 versus the blank or control group, ***P<0.005 versus the blank or control group, ##P<0.01 versus the underlined group, ###P<0.005 versus the underlined group. All raw Western blots data of this article are presented in Supplementary Figure S4. Values of all groups followed a Gaussian distribution.

FIGURE 5 | Correlation between sirtuin (SIRT)7 expression and immune infiltration levels of M1 macrophages and T cell exhaustion in breast cancer-luminal.. (B) Tumor infiltration of M1 macrophages and T cell exhaustion in breast cancer-luminal. The expression of SIRT7 (a: + + +, d: +). The expression of IRF5 (b: ++, e: –). The expression of PD1 (PDCD1) (c: + + +, f: +). The expression density of SIRT7, PD1, and IRF5 in breast cancer tissue was quantitated by scoring staining intensity,including negative (–) and weak (+) staining, moderate (++) and strong (+ + +) staining, respectively

Fig. 6. GRIM-19 stabilizes p53 in CRC cells through the SIRT7/PCAF/MDM2 axis. (A) Ratio of NAD + /NADH in HCT-8 cells transfected with the indicated siRNA or plasmids. (B) The half-life of MDM2 in HCT-8 cells transfected with the indicated plasmids was analyzed by Western blot. (C) Ubiquitylation levels of MDM2 in HCT-8 cells transfected with the indicated plasmids. Cells were treated with 20 μ M MG132 for 4 h before harvesting. (D) Western blot analyses of p53 expression in HCT-8 cells transfected with GRIM-19 and/or siSIRT7. (E) Ubiquitylation levels of p53 in HCT-8 cells transfected with GRIM-19 and/or siSIRT7. (F) Ubiquitylation levels of MDM2 in HCT-8 cells transfected with GRIM-19 and/or siSIRT7. (G) Acetylation levels of PCAF and MDM-2/PCAF interaction in HCT-8 cells transfected with GRIM-19 and/or siSIRT7. (H) Schematic of p53 activation by GRIM-19 through SIRT7-mediated MDM2 ubiquitination.