Caspase 1 Antibody - #DF6148

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产品: Caspase 1 抗体
货号: DF6148
描述: Rabbit polyclonal antibody to Caspase 1
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Rabbit
蛋白号: P29466
RRID: AB_2838115

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 50ul RMB¥ 1250 现货
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WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Caspase 1 Antibody detects endogenous levels of total Caspase 1.
RRID:
AB_2838115
引用格式: Affinity Biosciences Cat# DF6148, RRID:AB_2838115.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CASP-1; CASP1; CASP1_HUMAN; Caspase 1; Caspase-1 subunit p10; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1 beta converting enzyme; IL1B convertase; Interleukin 1 beta convertase; Interleukin 1B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45;

抗原和靶标

免疫原:

A synthesized peptide derived from human CASP1, corresponding to a region within C-terminal amino acids.

基因/基因ID:
CASP1(834)
描述:
Caspase-1, or interleukin-1 converting enzyme (ICE/ICEα), is a class I cysteine protease, which also includes caspases -4, -5, -11, and -12. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1 and interferon-γ inducing factor (IL-18) into their mature forms (1,2). Like other caspases, caspase-1 is proteolytically activated from a proenzyme to produce a tetramer of its two active subunits, p20 and p10. Caspase-1 has a large amino-terminal pro-domain that contains a caspase recruitment domain (CARD). Overexpression of caspase-1 can induce apoptosis (3). Mice deficient in caspase-1, however, have no overt defects in apoptosis but do have defects in the maturation of pro-IL-1β and are resistant to endotoxic shock (4,5). At least six caspase-1 isoforms have been identified, including caspase-1 α, β, γ, δ, ε and ζ (6). Most caspase-1 isoforms (α, β, γ and δ) produce products between 30-48 kDa and induce apoptosis upon over-expression. Caspase-1 ε typically contains only the p10 subunit, does not induce apoptosis and may act as a dominant negative. The widely expressed ζ isoform of caspase-1 induces apoptosis and lacks 39 amino-terminal residues found in the α isoform (6). Activation of caspase-1 occurs through an oligomerization molecular platform designated the inflammasome that includes caspase-5, Pycard/Asc, and NALP1 (7).

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

文献引用

1). 20(S)- Protopanaxadiol saponins isolated from Panax notoginseng target the binding of HMGB1 to TLR4 against inflammation in experimental ulcerative colitis. Phytotherapy Research, 2023 (PubMed: 37424151) [IF=6.1]
2). Transcranial focused ultrasound stimulation alleviates NLRP3-related neuroinflammation induced by ischemic stroke via regulation of the Nespas/miR-383-3p/SHP2 pathway. International immunopharmacology, 2025 (PubMed: 39616858) [IF=4.8]
3). Activation of CB2 receptor inhibits pyroptosis and subsequently ameliorates cecal ligation and puncture-induced sepsis. International immunopharmacology, 2021 (PubMed: 34364304) [IF=4.8]
4). SIRT1 regulates cigarette smoke extract‑induced alveolar macrophage polarization and inflammation by inhibiting the TRAF6/NLRP3 signaling pathway. Molecular medicine reports, 2025 (PubMed: 39635829) [IF=3.4]

Application: WB    Species: Rat    Sample: NR8383 cells

Figure 3. Ov-SIRT1 overexpression inhibits TRAF6/NLRP3 signaling activation in CSE-induced macrophages. (A) TRAF6, NLRP3 and cleaved caspase-1 protein expression in NR8383 cells. (B) Validation of TRAF6 binding ability to NLRP3. TRAF6 (C) protein and (D) mRNA and (E) TRAF6, NLRP3 and cleaved caspase-1 protein expression in NR8383 cells following TRAF6 overexpression. **P
5). Berberine attenuates uric acid-induced cell injury by inhibiting NLRP3 signaling pathway in HK-2 cells. Naunyn-Schmiedeberg's Archives of Pharmacology, 2023 (PubMed: 37193772) [IF=3.1]

Application: WB    Species: Human    Sample: HK-2 cells

Fig. 6 Effect of BBR on NLRP3 pathway in HK-2 cells using Western blot. A Western blot analyses of NLRP3, ASC, cl-Caspase1, Caspase1, IL-18, IL-1β, GSDMD, and β-actin. B–H Intensity of NLRP3, ASC, cl-Caspase1, Caspase1, IL-18, IL-1β, and GSDMD relative to β-actin. Values represent the means ± SD (n = 3). BBR, berberine (20 μM); UA, uric acid (20 mg/dL); cl-Caspase1, cleaved-Caspase1.
6). Polygalasaponin F ameliorates middle cerebral artery occlusion-induced focal ischemia / reperfusion injury in rats through inhibiting TXNIP/NLRP3 signaling pathway. Journal of neuroimmunology, 2024 (PubMed: 38198981) [IF=2.9]
7). Protective Effects of Cannabinoid Type 2 Receptor Activation Against Microglia Overactivation and Neuronal Pyroptosis in Sepsis-Associated Encephalopathy. Neuroscience, 2022 (PubMed: 35460837) [IF=2.9]
8). XueFu ZhuYu Decoction Alleviates Cardiopulmonary Bypass-Induced NLRP3 Inflammasome-Dependent Pyroptosis by Inhibiting IkB-α/NF-κB Pathway in Acute Lung Injury Rats. Evidence-based Complementary and Alternative Medicine, 2022 (PubMed: 36124015)

Application: IF/ICC    Species: Rat    Sample: lung tissues

Figure 6 XFZYD decreased pyroptosis in rat models with CPB-induced ALI. (a) Pyroptosis-related proteins in the lung tissues of rats with CPB-induced acute lung injury, including NLRP3, ASC, and caspase 1 were examined by immunofluorescence analysis. (b) Quantitative analysis of NLRP3, ASC, and caspase 1 expression. Scare bar = 100 μm. Values are expressed as mean ± SD. ∗P < 0.05; ∗∗P < 0.01, vs. CPB group.

Application: WB    Species: Rat    Sample: lung tissues

Figure 7 XFZYD inhibited the IKBα/NF-κB signaling in rat models with CPB-induced ALI. (a) The mRNA expression levels of NLRP3, ASC, GSDMD, and caspase 1 in CPB-induced lung injury rats treated with XFZYD, Ac-YVAD-CMK, and Bay-11-7082 were measured via RT-qPCR. (b and c) western blots were performed to determine NLRP3, ASC, Caspase-1 p20, Pro-GSDMD, GSDMD p30, IL-18, IL-1β p-P65, P65, p-IKBα, and IKBα levels in lung tissues of rats with CPB-induced acute lung injury. β-actin was used as a loading control for the blots. All the data were presented as the means ± SD. from independent experiments performed in triplicate. ∗P < 0.05; ∗∗P < 0.01, vs. CPB group.
9). Sulforaphane Ameliorates Limb Ischemia/Reperfusion-Induced Muscular Injury in Mice by Inhibiting Pyroptosis and Autophagy via the Nrf2-ARE Pathway. Evidence-Based Complementary and Alternative Medicine, 2022 (PubMed: 35600947)

Application: WB    Species: Mice    Sample:

Figure 4 (a) Immunofluorescent staining labeled autophagic markers Beclin-1 and LC3 was conducted to assess the extent of autophagy in each group of mice (scale bar = 50 μm); (b) Western blot was used to examine the expression of autophagy markers in the mouse muscle homogenate. The experiments were repeated three times. ∗p < 0.05, ∗∗p < 0.01.

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