CDCA8 Antibody - #DF6115

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产品: CDCA8 抗体
货号: DF6115
描述: Rabbit polyclonal antibody to CDCA8
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 31kDa; 31kD(Calculated).
蛋白号: Q53HL2
RRID: AB_2838082

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
预测:
Pig(86%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
CDCA8 Antibody detects endogenous levels of total CDCA8.
RRID:
AB_2838082
引用格式: Affinity Biosciences Cat# DF6115, RRID:AB_2838082.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BOR; BOREA_HUMAN; Borealin; CDCA8; Cell division cycle associated 8; cell division cycle associated protein 8; Cell division cycle-associated protein 8; Dasra B; Dasra-B; DasraB; FLJ10468; FLJ12042; hDasra B; hDasra-B; MESRGP; OTTHUMP00000004514; pluripotent embryonic stem cell related gene 3 protein; Pluripotent embryonic stem cell-related gene 3 protein;

抗原和靶标

免疫原:
Uniprot:

Q53HL2(BOREA_HUMAN) >>Visit HPA database.

基因/基因ID:
CDCA8(55143)
描述:
Borealin is a 280-amino acid protein that stabilizes the bipolar mitotic spindle. Borealin is part of a complex called Aurora B kinase that has essential regulatory roles at centromeres and the central spindle in cells undergoing mitosis. This complex makes up the enzymatic core of the chromosomal passenger complex (CPC). It is possible that the Borealin subunit directs the CPC to centromeric DNA. Deletion of Borealin delays mitotic progression and causes kinetochore-spindle to mis-attach. Deletion also causes an increase in bipolar spindles associated with ectopic asters. These extra poles, which form after chromosomes acquire their bipolar orientation, seriously disrupt chromosome allotment during anaphase. It is true that inappropriate chromosomal segregation and cell division may cause auneuploidy leading to cancer, so an abnormal expression of Borealin may lead to gastric cancer.
序列:
MAPRKGSSRVAKTNSLRRRKLASFLKDFDREVEIRIKQIESDRQNLLKEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEINKLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPPSKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVFKTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQRHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
100
Horse
100
Pig
86
Bovine
86
Sheep
86
Dog
86
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.

翻译修饰:

Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230. Phosphorylation (probably by CDK1) promotes targeting of the CPC to centromeric DNA.

Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.

细胞定位:

Nucleus>Nucleolus. Cytoplasm. Cytoplasm>Cytoskeleton>Spindle. Chromosome>Centromere.
Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

May form homooligomers and homodimers. Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB or AURKC; in the complex forms a triple-helix bundle-based subcomplex with INCENP and BIRC5. Interacts with SENP3, UBE2I and RANBP2. Interacts (phosphorylated) with SGO1 and SGO2; the association is dependent on CDK1.

蛋白家族:

The C-terminal region (aa 207-280) represents the dimerization motif.

Belongs to the borealin family.

文献引用

1). CDCA8, targeted by MYBL2, promotes malignant progression and olaparib insensitivity in ovarian cancer. American Journal of Cancer Research, 2023 (PubMed: 33575078) [IF=3.6]

Application: WB    Species: human    Sample: HGSOC and FT tissues

Figure 1.| CDCA8 is key gene high expressed and correlated to chemoresistance in ovarian cancer.(S and T) The mRNA and protein levels of CDCA8 in HGSOC and FT tissues.

Application: IHC    Species: human    Sample: tumor

Figure 2.| CDCA8 promotes the proliferation and motility of ovarian cancer. (H) Representative IHC staining of Ki-67 and CDCA8 in tumor tissues (400 ×), Scale bar: 25 µm.
2). Overexpression of CDCA8 predicts poor prognosis and promotes tumor cell growth in prostate cancer. Frontiers in Oncology, 2022 (PubMed: 35449575) [IF=3.5]

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