产品: Grp75 抗体
货号: DF6108
描述: Rabbit polyclonal antibody to Grp75
应用: WB IHC
反应: Human, Mouse, Rat
预测: Bovine, Sheep, Rabbit
分子量: 74kDa; 74kD(Calculated).
蛋白号: P38646
RRID: AB_2838076

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Sheep(100%), Rabbit(100%)
克隆:
Polyclonal
特异性:
Grp75 Antibody detects endogenous levels of total Grp75.
RRID:
AB_2838076
引用格式: Affinity Biosciences Cat# DF6108, RRID:AB_2838076.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

75 kDa glucose regulated protein; 75 kDa glucose-regulated protein; CSA; Glucose Regulated Protein; Grp 75; GRP-75; GRP75; GRP75_HUMAN; Heat shock 70 kDa protein 9; Heat shock 70kD protein 9; heat shock 70kDa protein 9; Heat shock 70kDa protein 9B; Heat shock protein 74 kDa A; Heat shock protein A; Heat shock protein cognate 74; Hsc74; Hsp74; Hsp74a; HSPA9; Hspa9a; HSPA9B; MGC4500; mitochondrial; Mortalin 2; Mortalin; Mortalin perinuclear; Mortalin2; MOT 2; MOT; MOT2; Mthsp70; p66 mortalin; P66 MOT; PBP74; Peptide binding protein 74; Peptide-binding protein 74; Stress 70 protein mitochondrial; Stress 70 protein mitochondrial precursor; Stress-70 protein;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat-inducible (1,2). This protein is essential for transporting many mitochondrial proteins from the cytoplasm to mitochondria (3). Grp75 inactivates the tumor suppressor p53 (4). Studies found that Grp75 is overexpressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (5). Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts (6).
序列:
MISASRAAAARLVGAAASRGPTAARHQDSWNGLSHEAFRLVSRRDYASEAIKGAVVGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTADGERLVGMPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDIKNVPFKIVRASNGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGLDKSEDKVIAVYDLGGGTFDISILEIQKGVFEVKSTNGDTFLGGEDFDQALLRHIVKEFKRETGVDLTKDNMALQRVREAAEKAKCELSSSVQTDINLPYLTMDSSGPKHLNMKLTRAQFEGIVTDLIRRTIAPCQKAMQDAEVSKSDIGEVILVGGMTRMPKVQQTVQDLFGRAPSKAVNPDEAVAIGAAIQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGEREMAGDNKLLGQFTLIGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMVKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLARKDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Rabbit
100
Pig
0
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P38646 作为底物

Site PTM Type Enzyme
S3 Phosphorylation
S5 Phosphorylation
T22 Phosphorylation
S29 Phosphorylation
Y46 Phosphorylation
T62 Phosphorylation P33981 (TTK)
S65 Phosphorylation P33981 (TTK)
K76 Ubiquitination
T86 Phosphorylation
T87 Phosphorylation
K106 Ubiquitination
T111 Phosphorylation
Y118 Phosphorylation
T120 Phosphorylation
K121 Acetylation
K121 Ubiquitination
Y128 Phosphorylation
K135 Acetylation
K135 Ubiquitination
K138 Acetylation
K138 Ubiquitination
K143 Acetylation
K143 Ubiquitination
S148 Phosphorylation
K159 Acetylation
Y161 Phosphorylation
S162 Phosphorylation
S164 Phosphorylation
T177 Phosphorylation
Y181 Phosphorylation
T185 Phosphorylation
K187 Ubiquitination
T192 Phosphorylation
Y196 Phosphorylation
S200 Phosphorylation
T205 Phosphorylation
K206 Acetylation
K206 Ubiquitination
S212 Phosphorylation
R218 Methylation
Y230 Phosphorylation
K234 Acetylation
K234 Ubiquitination
S253 Phosphorylation
K265 Ubiquitination
S266 Phosphorylation
K288 Acetylation
K288 Ubiquitination
K291 Acetylation
K300 Acetylation
K300 Ubiquitination
R307 Methylation
T333 Phosphorylation
S336 Phosphorylation
K345 Acetylation
T356 Phosphorylation
R360 Methylation
T362 Phosphorylation
K368 Acetylation
K368 Ubiquitination
S376 Phosphorylation
K377 Acetylation
S378 Phosphorylation
T390 Phosphorylation
K394 Acetylation
K394 Ubiquitination
T398 Phosphorylation
R405 Methylation
S408 Phosphorylation
T462 Phosphorylation
T480 Phosphorylation
C487 S-Nitrosylation
K498 Acetylation
T504 Phosphorylation
R513 Methylation
S533 Phosphorylation
K555 Ubiquitination
K567 Acetylation
Y568 Phosphorylation
K595 Acetylation
K600 Acetylation
C608 S-Nitrosylation
K610 Acetylation
K612 Acetylation
K612 Ubiquitination
S616 Phosphorylation
R624 Methylation
S627 Phosphorylation
S638 Phosphorylation
S639 Phosphorylation
K646 Acetylation
K646 Ubiquitination
Y652 Phosphorylation
K653 Methylation
K653 Ubiquitination
S662 Phosphorylation
K671 Acetylation
K675 Acetylation
K678 Acetylation

研究背景

功能:

Chaperone protein which plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis. Interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU. Regulates erythropoiesis via stabilization of ISC assembly. May play a role in the control of cell proliferation and cellular aging (By similarity).

细胞定位:

Mitochondrion. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts strongly with the intermediate form of FXN and weakly with its mature form. Interacts with HSCB. Associates with the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MICOS10/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1, MTX2 and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. Interacts with DNLZ, the interaction is required to prevent self-aggregation. Interacts with TESPA1. Interacts with PDPN. Interacts with NFU1, NFS1 and ISCU.

蛋白家族:

Belongs to the heat shock protein 70 family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

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