IGF1 Antibody - #DF6096

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产品: IGF1 抗体
货号: DF6096
描述: Rabbit polyclonal antibody to IGF1
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白ID: P05019
RRID: AB_2838064

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
IGF1 Antibody detects endogenous levels of total IGF1.
RRID:
AB_2838064
引用格式: Affinity Biosciences Cat# DF6096, RRID:AB_2838064.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

IBP1; IGF I; IGF IA; IGF IB; IGF-I; Igf1; IGF1_HUMAN; IGF1A; IGFI; IGFIA; Insulin like growth factor 1 (somatomedin C); Insulin like growth factor 1; Insulin like growth factor IA; Insulin like growth factor IB; Insulin-like growth factor I; Mechano growth factor; MGF; OTTHUMP00000195080; OTTHUMP00000195081; OTTHUMP00000195082; OTTHUMP00000195083; OTTHUMP00000195084; Somatomedia C; Somatomedin C; Somatomedin-C;

抗原和靶标

免疫原:

A synthesized peptide derived from human IGF1, corresponding to a region within the internal amino acids.

基因/基因ID:
IGF1(3479)
描述:
IGF1, also named as IBP1, MGF, IGF-IA and Somatomedin-C, belongs to the insulin family. IGF1 is structurally and functionally related to insulin but have a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. The antibody is specific to isoform IGF-1A.

研究领域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

文献引用

1). Dual-action bisphosphonate hydrogel preserves cortical bone integrity and restores healing cascade in osteoporotic fracture-related infection. Nature communications, 2026 (PubMed: 41519832) [IF=16.6]
2). Tumor-associated macrophages/C-X-C motif chemokine ligand 1 promotes breast cancer autophagy-mediated chemoresistance via IGF1R/STAT3/HMGB1 signaling. Cell death & disease, 2024 (PubMed: 39394189) [IF=8.1]

Application: WB    Species: Mouse    Sample: MDA-MB-231 cells

Fig. 5: Identification of IGF1/IGF1R as a downstream signaling for CXCL1-induced autophagy.A The heatmap and Venn diagram analyses were conducted using data from a high-throughput quantitative PCR chip to evaluate differences among the indicated four groups, with a focus on IGF1 as the primary target. B Western blotting analysis was performed to evaluate the impact of varying concentrations of CXCL1 (0–50 ng/mL), CXCL1 overexpression, or CXCL1 knockdown on the expression levels of IGF1 and its receptor IGF1R in MDA-MB-231 cells. C Immunofluorescence was utilized to examine the expression and distribution of IGF1R with 20 ng/mL CXCL1 alone or its combination with 40 ng/mL IGF1 (scale bars indicate 20 μm). D, E mRFP-GFP-LC3 reporter assay was conducted to investigate the effect of 40 ng/mL IGF1 or IGF1R silence on CXCL1-mediated autophagy in MDA-MB-231 cells (n = 5, scale bars indicate 20 μm). F CoIP analysis was conducted to assess the effect of CXCL1 treatment (20 ng/mL) on protein interaction between IGF1 and IGF1R in MDA-MB-231 cells. G, H Western blotting assay was utilized to examine whether the change of IGF1R expression was affected by proteasome degradation. CXCL1 dose was 20 ng/mL, IGF1 dose was 40 ng/mL, CHX dose was 10 μM, and MG132 dose was 10 μM. Values are presented as Mean ± SD, n = 3 unless otherwise indicated, *P 
3). GPNMB and ATP6V1A interact to mediate microglia phagocytosis of multiple types of pathological particles. Cell reports, 2025 (PubMed: 39992792) [IF=7.5]
4). Cathepsin S activity controls chronic stress-induced muscle atrophy and dysfunction in mice. Cellular and Molecular Life Sciences, 2023 (PubMed: 37589754) [IF=6.2]

Application: WB    Species: Mouse    Sample:

Fig. 4 CTSS deficiency ameliorated stress-related anabolic and catabolic molecular alterations. a–e: Representative immunoblotting images and quantitative data for CTSS, IGF-1, IRS-2, p-PI3K, p-Akt, p-mTOR, p-FoxO1α, MuRF-1, MAFbx1, PGC-1α, PPAR-γ, C-caspase-3, and Bcl-2 in GAS muscles at Day 14 after stress (n = 3). Data are mean ± SEM, and p-values were determined by a one-way ANOVA followed by Bonferroni post hoc tests (b–e). CW: CTSS+/+ control mice, CK: CTSS−/− control mice, SW: 14-day-stressed CTSS+/+ mice, SK: 14-day-stressed CTSS−/− mice. *p 
5). An IGF1-expressing endometrial stromal cell population is associated with human decidualization. BMC Biology, 2022 (PubMed: 36482461) [IF=5.4]

Application: IHC    Species: Human    Sample:

Fig. 7 The aberrant ratio of IGF1+SC to IGF1R+SC is observed in repeated implantation failure patients. A Cell phone heatmap showed the interaction of cells. The number indicates the number of interactions. B Heat map showing relative expression (z-score) of IGF1 and IGF1R in the total Rem_SC (Rem_SC and dRem_SC) from Endometrium and Decidua. C Heat map showing relative expression (z-score) of IGF1 and IGF1R in the stroma cells from Ctrl and RIF. D, E The expression of IGF1 and IGF1R in the endometrial stroma from controls and RIF patients at the WOI time were analyzed by immunohistochemistry (n = 3 for each group). Scale bar, 20 μm. F General diagram of decidualization process. During the time of WOI, the heterogeneity of stromal cells determines different division of labor. More specifically, IGF1+ stromal cells (Rem) start endometrial decidualization (mainly IGF1R+ stromal cells, i.e., dRem and dSec) by crosstalking with EVT and immune cells, including AREG+ NK cells; IL1B+ stromal cells (dSec) participate in the establishment and maintenance of decidua hemostasis.
6). Cardiomyocyte protective effects of thyroid hormone during hypoxia/reoxygenation injury through activating of IGF‐1‐mediated PI3K/Akt signalling. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021 (PubMed: 33724692) [IF=5.3]

Application: WB    Species: mouse    Sample: cardiomyocytes

FIGURE 6| T3 protected cardiomyocytes from H/R-induced apoptosis through PI3K/Akt signalling pathway (A and B). A, The expression of Akt, P-Akt, PI3K, P-Akt, IGF-1, IGF-1R; β-actin protein expression was used for normalization and (B) representative bands quantified in the corresponding bar graph.
7). Kisspeptin mediates the impact of chronic psychological stress on reproductive and metabolic dysregulation in polycystic ovary syndrome: evidence from human and rat models. Journal of ovarian research, 2025 (PubMed: 41430614) [IF=4.0]
8). WISP2/IGF-1 promotes the survival of DSCs and impair the cytotoxicity of decidual NK cells. REPRODUCTION, 2021 (PubMed: 33561006) [IF=3.7]
9). Capsaicin reduces blood glucose and prevents prostate growth by regulating androgen, RAGE/IGF-1/Akt, TGF-β/Smad signalling pathway and reversing epithelial-mesenchymal transition in streptozotocin-induced diabetic mice. Naunyn-Schmiedeberg's archives of pharmacology, 2024 (PubMed: 38700794) [IF=3.1]
10). Novel_circ_003686 regulates the osteogenic differentiation of MSCs in patients with myeloma bone disease through miR-142-5p/IGF1 axis. Journal of bone oncology, 2023 (PubMed: 38021072) [IF=3.1]

Application: WB    Species: Human    Sample:

Fig. 5 Regulatory relationship between miR-142-3p and IGF1. (A) Online TargetScan (https://www.targetscan.org/vert_72/) suggests that miR-142-3p may bind to 3′ untranslated region (3′UTR) of IGF1 mRNA. (B) The binding of miR-142-3p to IGF1 was verified by dual-luciferase reporter assay. (C) IGF1 mRNA expression after miR-142-3p-mimic treatment was examined by RT-PCR analysis. (D-E) The IGF1 was silencing by si-IGF1-1, si-IGF1-2 or si-IGF1-3 in MBD-BMSC, and the protein expression was observed by WB. (F) IGF1 mRNA expression after IGF1 was silenced was examined by RT-PCR. * P < 0.05, ** P < 0.01 and *** P < 0.001.
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