PPAR gamma Antibody - #DF6073

Figure 3. Leptin deficiency decreases SREBP1 via upregulation of PPAR-γ in NPC cells. (A) Western blot analysis of PPAR-γ and SREBP1 in leptin-depleted NPC cells in the absence or presence of GW9662. (B,C) The levels of TG and cholesterol in the indicated NPC cells with GW9662 treatment were measured. All data were obtained from three independent experiments. Data are presented as the mean ± SD. *: p < 0.05; **: p < 0.01; ***: p < 0.001.

FIGURE 2 Expression level of lipid metabolism cytokine in MCD-fed mice. (A,B) The mRNA and protein expression levels of FASN and SERBP-1C were increased. PPAR-α and ACOX-1 expression were decreased in NAFLD liver tissues. (Data are represented by at least three independent mean ± SD, *p < 0.05, **p < 0.01 vs normal group).

Fig. 3. E4BP4 mediates the actions of glucocorticoid in adipocyte formation. (a, b) The adipogenic phenotypes of 3T3-L1 cells transfected with pCDNA3.1-E4BP4 or control after induction by MI for 6 days were assessed by ORO staining. (b) Triglyceride accumulation was quantified and normalized to protein amount. (c) The mRNA levels of PPARg2, aP2, adiponectin, and LPL at day 6 were detected by qPCR. (d) The protein levels of PPARg and aP2 at day 6 were measured by Western blot. (e) The adipogenic phenotypes of 3T3-L1 cells transfected with siE4BP4 or control after being induced by DEX only for 6 days were assessed by ORO staining. (f) Triglyceride accumulation was quantified and normalized to protein amount. (g) qPCR analysis of PPARg2, aP2, adiponectin, and LPL. (h) Western blot analysis of PPARg and aP2. The results are expressed as mean ± SD (n ¼ 3); *P < 0.05 and **P < 0.01 indicate significant difference from the control.