PIK3CG Antibody - #DF6069

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产品: PIK3CG 抗体
货号: DF6069
描述: Rabbit polyclonal antibody to PIK3CG
应用: WB IF/ICC
文献验证: IF/ICC
反应: Human, Mouse, Rat
蛋白号: P48736
RRID: AB_2838037

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
PIK3CG Antibody detects endogenous levels of total PIK3CG.
RRID:
AB_2838037
引用格式: Affinity Biosciences Cat# DF6069, RRID:AB_2838037.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

1 phosphatidylinositol 3 kinase; 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma; 5-bisphosphate 3-kinase catalytic subunit gamma isoform; p110 gamma; p110gamma; p120 PI3K; p120-PI3K; Phosphatidylinositol 3 kinase catalytic 110 kD gamma; Phosphatidylinositol 3 kinase gamma, p110 gamma; Phosphatidylinositol 3 kinase, catalytic, gamma polypeptide; Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit gamma; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma isoform; Phosphatidylinositol-4; Phosphoinositide 3 kinase catalytic gamma polypeptide; Phosphoinositide 3 kinase gamma catalytic subunit; PI3 kinase p110 subunit gamma; PI3-kinase subunit gamma; PI3CG; PI3K; PI3K-gamma; PI3Kgamma; PIK3; Pik3cg; PK3CG_HUMAN; PtdIns-3-kinase subunit gamma; PtdIns-3-kinase subunit p110-gamma; Serine/threonine protein kinase PIK3CG;

抗原和靶标

免疫原:

A synthesized peptide derived from human PIK3CG, corresponding to a region within C-terminal amino acids.

基因/基因ID:
PIK3CG(5294)
描述:
Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).

研究领域

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

文献引用

1). Innate/Inflammatory Bioregulation of Surfactant Protein D Alleviates Rat Osteoarthritis by Inhibiting Toll-Like Receptor 4 Signaling. Frontiers in Immunology, 2022 (PubMed: 35865531) [IF=5.7]

Application: IF/ICC    Species: Rat    Sample: chondrocytes

Figure 8 Suppression of inflammatory response and the PI3K and NF-κB pathways by SP-D treatment in vitro. (A, B) Chondrocytes were pre-incubated with rhSP-D and transfection of pcDNA3.1-SP-D plasmid for 2 h before LPS co-treatment for 24 h. The concentrations of IL-1β and TNF-α were determined by ELISA. Data were expressed as mean ± SEM (n = 3). ***P < 0.001 vs. control group; ###P < 0.001 vs. LPS group. (C–F) Immunofluorescence with antibodies to PI3K and p65 in chondrocytes, and the fluorescence images of PI3K and p65-Tracker Red in chondrocytes. The IOD was quantified according to immunofluorescence. Data were expressed as mean ± SEM (n = 3). *P < 0.05 vs. LPS group; ##P < 0.01 and ###P < 0.001 vs. LPS + rhSP-D group.

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