PCNA Antibody - #DF6067

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产品: PCNA 抗体
货号: DF6067
描述: Rabbit polyclonal antibody to PCNA
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: P12004
RRID: AB_2838035

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
PCNA Antibody detects endogenous levels of total PCNA.
RRID:
AB_2838035
引用格式: Affinity Biosciences Cat# DF6067, RRID:AB_2838035.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; OTTHUMP00000030189; OTTHUMP00000030190; PCNA; Pcna/cyclin; PCNA_HUMAN; PCNAR; Polymerase delta accessory protein; Proliferating cell nuclear antigen; wu:fa28e03; wu:fb36g03;

抗原和靶标

免疫原:

A synthesized peptide derived from human PCNA, corresponding to a region within the internal amino acids.

基因/基因ID:
PCNA(5111)
描述:
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation and PCNA (PC10) Mouse mAb has been used to assess PCNA levels in hundreds of scientific studies.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Genetic Information Processing > Replication and repair > DNA replication.

· Genetic Information Processing > Replication and repair > Base excision repair.

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Genetic Information Processing > Replication and repair > Mismatch repair.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

文献引用

1). Artificial nerve graft constructed by coculture of activated Schwann cells and human hair keratin for repair of peripheral nerve defects. Neural Regeneration Research, 2023 (PubMed: 36255001) [IF=5.9]

Application: IF/ICC    Species: Human    Sample:

Figure 4: The bioactive artificial nerve promotes Schwann cell proliferation in the injured area after sciatic nerve injury.Control group: direct suture of sciatic nerve transection; HHK group: artificial nerve bridging was used after sciatic nerve transection. (A) The number of S100-positive cells (green, stained by Dylight 488) was detected by immunohistochemical staining. The number of S100-positive cells in the control group was less than that in the HHK group. Scale bars: 200 μm (upper), 50 μm (lower). (B) Immunofluorescence staining was used for S100 (green, stained by Dylight 488) and PCNA (red, stained by Fluor594). The numbers of S100- and PCNA-positive cells in the control group were less than those in the HHK group. Scale bars: 100 μm. Data are expressed as mean ± SD (n = 6). ***P < 0.001 (independent sample t-test). HHK: Human hair keratin; PCNA: proliferating cell nuclear antigen.
2). 加载活化许旺细胞的人发角蛋白骨架构建新型人工神经移植物可桥接修复周围神经缺损. 中国神经再生研究(英文版), 2023 (PubMed: 36255001) [IF=5.9]

Application: IF/ICC    Species: Human    Sample:

Figure 4: The bioactive artificial nerve promotes Schwann cell proliferation in the injured area after sciatic nerve injury.Control group: direct suture of sciatic nerve transection; HHK group: artificial nerve bridging was used after sciatic nerve transection. (A) The number of S100-positive cells (green, stained by Dylight 488) was detected by immunohistochemical staining. The number of S100-positive cells in the control group was less than that in the HHK group. Scale bars: 200 μm (upper), 50 μm (lower). (B) Immunofluorescence staining was used for S100 (green, stained by Dylight 488) and PCNA (red, stained by Fluor594). The numbers of S100- and PCNA-positive cells in the control group were less than those in the HHK group. Scale bars: 100 μm. Data are expressed as mean ± SD (n = 6). ***P < 0.001 (independent sample t-test). HHK: Human hair keratin; PCNA: proliferating cell nuclear antigen.
3). A novel long non-coding RNA LINC00355 promotes proliferation of lung adenocarcinoma cells by down-regulating miR-195 and up-regulating the expression of CCNE1. CELLULAR SIGNALLING, 2020 (PubMed: 31689506) [IF=4.4]
4). The Inhibitory Effect of Eplerenone on Cell Proliferation in the Contralateral Kidneys of Rats with Unilateral Ureteral Obstruction. NEPHRON, 2017 (PubMed: 28402979) [IF=2.3]

Application: WB    Species: Rat    Sample:

Fig. 3. Inhibitory effect of eplerenone on proliferation in the contralateral kidney of rats with UUO. A The expression of PCNA in the contralateral kidney of the rats with UUO. IHC ×400; scale bar = 20 μm. Only a few PCNA-positive cells were observed in kidneys in the sham group. In contralateral kidney of UUO rats, PCNA-positive cells were observed mainly in the tubular epithelial cells; some of them were also observed in interstitium. Ba Western blot analysis of PCNA in the contralateral kidney. Bb Protein expression in treated rats in comparison with the sham group. Data represent the mean ± SD of data from 6 rats in each group vs. the sham group,

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