SirT1 Antibody - #DF6033

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产品: SirT1 抗体
货号: DF6033
描述: Rabbit polyclonal antibody to SirT1
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat, Monkey
蛋白号: Q96EB6
RRID: AB_2838007

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
SirT1 Antibody detects endogenous levels of total SirT1.
RRID:
AB_2838007
引用格式: Affinity Biosciences Cat# DF6033, RRID:AB_2838007.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

75SirT1; hSIR2; hSIRT1; HST2, S. cerevisiae, homolog of; NAD dependent deacetylase sirtuin 1; NAD dependent protein deacetylase sirtuin 1; OTTHUMP00000198111; OTTHUMP00000198112; Regulatory protein SIR2 homolog 1; SIR1_HUMAN; SIR2; SIR2 like 1; SIR2 like protein 1; SIR2, S.cerevisiae, homolog-like 1; SIR2-like protein 1; SIR2ALPHA; SIR2L1; Sirt1; SirtT1 75 kDa fragment; Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); Sirtuin 1; Sirtuin type 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human SirT1, corresponding to a region within C-terminal amino acids.

基因/基因ID:
SIRT1(23411)
描述:
The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARγ (7), and the PPARγ coactivator-1α (PGC-1α) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, since it is phosphorylated on Ser27 and Ser47 in vivo, however, the function of these phosphorylation sites has not yet been determined (9).

研究领域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

文献引用

1). Manganese-based nanozymes as broad-spectrum antioxidants against cisplatin-induced skeletal muscle atrophy. Chemical Engineering Journal, 2025 [IF=13.3]
2). Fyn deficiency inhibits oxidative stress by decreasing c-Cbl-mediated ubiquitination of Sirt1 to attenuate diabetic renal fibrosis. Metabolism, 2023 (PubMed: 36538986) [IF=10.8]
3). Chlorogenic acid exerts neuroprotective effect against hypoxia-ischemia brain injury in neonatal rats by activating Sirt1 to regulate the Nrf2-NF-κB signaling pathway. Cell Communication and Signaling, 2022 (PubMed: 35689269) [IF=8.4]

Application: WB    Species: Rat    Sample: primary cortical neurons

Fig. 9 Chlorogenic acid regulates the Nrf2-NF-κB signaling pathway by activating Sirt1 in primary cortical neurons. a Western blot evaluation of the protein levels of Sirt1, IκB, HO-1, Nrf2, NF-κB 24h after OGD. b–f Quantification of western blot data of Sirt1, IκB, HO-1, Nrf2, NF-κB. ∗∗P < 0.01 and ∗∗∗P < 0.001 vs. the control group. #P < 0.05, ##P < 0.01 and ###P < 0.001 vs. the OGD group. n = 3
4). Polyoxometalates Ameliorate Metabolic Dysfunction-Associated Steatotic Liver Disease by Activating the AMPK Signaling Pathway. International journal of nanomedicine, 2024 (PubMed: 39479173) [IF=8.0]
5). Hepatoprotective effects of polysaccharide from Morchella esculenta are associated with activation of the AMPK/Sirt1 signaling pathway in mice with NAFLD. International journal of biological macromolecules, 2025 (PubMed: 39884630) [IF=7.7]
6). Metformin suppresses cardiac fibroblast proliferation under high-glucose conditions via regulating the mitochondrial complex I protein Grim-19 involved in the Sirt1/Stat3 signaling pathway. Free radical biology & medicine, 2023 (PubMed: 37353174) [IF=7.1]

Application: WB    Species: Mouse    Sample:

Fig. 1. Metformin suppressed cardiac fibrosis in mice with diabetes. (a) Hematoxylin and eosin staining; (b) Masson trichrome staining; (c) α-SMA of the myocardium; and (d) Changes in the protein levels of Sirt1, p-Stat3 and Grim-19 in mice with diabetes. (e, f) Expression of Grim-19 in different tissues. (g) Venn diagram of genes regulated by metformin and Grim-19 by searching the TCMSP database. (h) GO enrichment analysis for metformin and Grim-19-related pathways. The y-axis represents the enriched GO terms; the x-axis represents the expression levels of metformin- and Grim-19-related mRNAs enriched in GO terms. Data are expressed as the mean ± standard deviation (n = 6 per group). *P < 0.05, **P < 0.01, ***P < 0.001.
7). β-patchoulene improves lipid metabolism to alleviate non-alcoholic fatty liver disease via activating AMPK signaling pathway. BIOMEDICINE & PHARMACOTHERAPY, 2021 (PubMed: 33341045) [IF=6.9]

Application: WB    Species: Human    Sample: L02 cell

Fig. 6. β-PAE promotes the expression of hepatic lipid oxidation-related proteins and genes in HFD-fed rats. (A–G) Western blot analysis on the expression of SIRT1, PGC-1α, PPARα, FGF21, CPT-1a and ACOX1; (H–K) The mRNA expression of SIRT1, PPARα, CPT-1a and ACOX1. Data are presented as the mean ± SD (n = 6~8). ##p < 0.01 vs. NC group; *p < 0.05, **p < 0.01 vs. Model group.
8). TMF inhibits extracellular matrix degradation by regulating the C/EBPβ/ADAMTS5 signaling pathway in osteoarthritis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38554527) [IF=6.9]

Application: WB    Species: Human    Sample: C28/I2 cells

Fig. 6. TMF inhibited ECM degradation by activating SIRT1 expression. (A–B) IHC assays were used to detect the altered expression of SIRT1. (C–J) Western blotting assays were used to detect the protein expression of Aggrecan, ADAMTS5, C/EBPβ, p-C/EBPβ, caspase3, cleaved-caspase3, SIRT1, and FOXO3a in EX527-treated C28/I2 cells. (K–L) The apoptosis rate was determined by a flow cytometer.

Application: IHC    Species: Human    Sample: C28/I2 cells

Fig. 6. TMF inhibited ECM degradation by activating SIRT1 expression. (A–B) IHC assays were used to detect the altered expression of SIRT1. (C–J) Western blotting assays were used to detect the protein expression of Aggrecan, ADAMTS5, C/EBPβ, p-C/EBPβ, caspase3, cleaved-caspase3, SIRT1, and FOXO3a in EX527-treated C28/I2 cells. (K–L) The apoptosis rate was determined by a flow cytometer.
9). White adipose tissue, a novel antirheumatic target: Clues from its secretory capability and adipectomy-based therapy. British journal of pharmacology, 2024 (PubMed: 38644540) [IF=6.8]
10). Insights into the mechanism of action of pterostilbene against influenza A virus-induced acute lung injury. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38583346) [IF=6.7]
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