SirT1 Antibody - #DF6033

Figure 1. SIRT1 is downregulated in Fibrotic Bladder Tissue and Activated Fibroblasts. A) t‐distributed stochastic neighbor embedding (t‐SNE) visualization illustrating the distribution of five distinct cell populations isolated from murine bladder tissues in both control and BOO‐induced groups (n = 3 per group). Fibro, fibroblast; UC, urothelial cell; IC, immune cell; SMC, smooth muscle cell; SC, Schwann cell. B) Gene expression patterns of marker genes on t‐SNE plots: fibroblasts (Pdgfra), urothelial cells (Upk1a), immune cells (Ptprc), smooth muscle cells (Acta2), and Schwann cells (Cdh19). C) Dot plot summarizing the top five marker genes in each cluster. D) Gene Set Enrichment Analysis (GSEA) highlighting biological processes significantly associated with marker genes derived from each cell population. E) Feature plot analysis reveals pronounced downregulation of Sirt1expression in fibrotic bladder tissue. F,G) Bulk RNA sequencing analysis of a rat BOO model identifies differentially expressed genes (DEGs) enriched in pathways related to fibroblast proliferation, concurrent with reduced Sirt1expression. H) Representative histopathological images of bladder sections stained with hematoxylin and eosin (H&E) and Sirius red, comparing control and BOO groups (scale bar = 100 µm). Data are presented as the mean ± standard deviation (SD) (n = 3). I) Immunofluorescence staining confirms predominant SIRT1 expression within PDGFRA⁺ fibroblasts and demonstrates significant reduction following BOO induction (scale bar = 20 µm). J) Western blot analysis shows decreased SIRT1 protein levels accompanied by elevated expression of collagen‐I (COL‐1) and fibronectin (FN) in fibrotic bladders. K) Integrated analysis of the publicly available transcriptomic dataset GSE15871 (Gene Expression Omnibus) reveals significant downregulation of Sirt1in TGF‐β1‐stimulated fibroblasts relative to controls. Data are presented as the mean ± SD (n = 3). L) Validation of SIRT1 suppression in cultured mouse bladder fibroblasts following TGF‐β1 treatment. Data are presented as the mean ± SD (n = 3). Statistical analysis was performed using Student's t‐test.

Fig. 9 Chlorogenic acid regulates the Nrf2-NF-κB signaling pathway by activating Sirt1 in primary cortical neurons. a Western blot evaluation of the protein levels of Sirt1, IκB, HO-1, Nrf2, NF-κB 24h after OGD. b–f Quantification of western blot data of Sirt1, IκB, HO-1, Nrf2, NF-κB. ∗∗P < 0.01 and ∗∗∗P < 0.001 vs. the control group. #P < 0.05, ##P < 0.01 and ###P < 0.001 vs. the OGD group. n = 3

Fig. 1. Metformin suppressed cardiac fibrosis in mice with diabetes. (a) Hematoxylin and eosin staining; (b) Masson trichrome staining; (c) α-SMA of the myocardium; and (d) Changes in the protein levels of Sirt1, p-Stat3 and Grim-19 in mice with diabetes. (e, f) Expression of Grim-19 in different tissues. (g) Venn diagram of genes regulated by metformin and Grim-19 by searching the TCMSP database. (h) GO enrichment analysis for metformin and Grim-19-related pathways. The y-axis represents the enriched GO terms; the x-axis represents the expression levels of metformin- and Grim-19-related mRNAs enriched in GO terms. Data are expressed as the mean ± standard deviation (n = 6 per group). *P < 0.05, **P < 0.01, ***P < 0.001.

Fig. 6. TMF inhibited ECM degradation by activating SIRT1 expression. (A–B) IHC assays were used to detect the altered expression of SIRT1. (C–J) Western blotting assays were used to detect the protein expression of Aggrecan, ADAMTS5, C/EBPβ, p-C/EBPβ, caspase3, cleaved-caspase3, SIRT1, and FOXO3a in EX527-treated C28/I2 cells. (K–L) The apoptosis rate was determined by a flow cytometer.

Fig. 6. TMF inhibited ECM degradation by activating SIRT1 expression. (A–B) IHC assays were used to detect the altered expression of SIRT1. (C–J) Western blotting assays were used to detect the protein expression of Aggrecan, ADAMTS5, C/EBPβ, p-C/EBPβ, caspase3, cleaved-caspase3, SIRT1, and FOXO3a in EX527-treated C28/I2 cells. (K–L) The apoptosis rate was determined by a flow cytometer.

Fig. 6. β-PAE promotes the expression of hepatic lipid oxidation-related proteins and genes in HFD-fed rats. (A–G) Western blot analysis on the expression of SIRT1, PGC-1α, PPARα, FGF21, CPT-1a and ACOX1; (H–K) The mRNA expression of SIRT1, PPARα, CPT-1a and ACOX1. Data are presented as the mean ± SD (n = 6~8). ##p < 0.01 vs. NC group; *p < 0.05, **p < 0.01 vs. Model group.