EPHA4 Antibody - #AF5496

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产品: EPHA4 抗体
货号: AF5496
描述: Rabbit polyclonal antibody to EPHA4
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P54764
RRID: AB_2837975

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
EPHA4 Antibody detects endogenous levels of total EPHA4.
RRID:
AB_2837975
引用格式: Affinity Biosciences Cat# AF5496, RRID:AB_2837975.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cek 8; CEK8; EK8; eph receptor a4; EPH-like kinase 8; EPHA4; EPHA4_HUMAN; Ephrin type-A receptor 4; HEK 8; hEK8; Receptor protein-tyrosine kinase HEK8; Sek 1; SEK; TYRO 1 protein tyrosine kinase; TYRO1; Tyrosine-protein kinase receptor SEK; Tyrosine-protein kinase TYRO1;

抗原和靶标

免疫原:

A synthesized peptide derived from human EPHA4, corresponding to a region within the internal amino acids.

基因/基因ID:
EPHA4(2043)
描述:
Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.

研究领域

· Organismal Systems > Development > Axon guidance.   (View pathway)

文献引用

1). Nogo-A-Δ20/EphA4 interaction antagonizes apoptosis of neural stem cells by integrating p38 and JNK MAPK signaling. JOURNAL OF MOLECULAR HISTOLOGY, 2021 (PubMed: 33555537) [IF=2.9]

Application: IF/ICC    Species: rat    Sample: neural stem cells

Fig. 1 Co-expression of Nogo-A-Δ20 and EphA4 in neural stem cells. a The monoclonal antibody (mAb) A563 against aa 563–627 (amino acids (aa) 563–627) of rat Nogo-A-NiG-Δ20, which is the main inhibitory region of rat Nogo-A. Immunofuorescence staining demonstrate that NSCs spheres are both positive for A563 and EphA4. Co- expression regions are labeled in yellow in the merged images. Staining of Hoechst 33,258 to show the nuclei. Scale bar 50 μm. b We dissociated and plated the neurospheres as single cells and performed Nogo-A and EphA4 double immunostaining. Colocalization are presented in yellow in the merged images. These were colocalization restricted to NSCs growing in the presence of growth factors. Scale bar 100 μm. c–f Western blot analysis detecting NogoA (~135  kDa), EphA4 (~110  kDa), GFAP (~50  kDa) and β-actin (~43 kDa) proteins in supernatants collected from lysis solutions of neurospheres. All bands presented are representative images of at three independent experiments. M marker. g The diagram represents the three inhibitory regions of rat Nogo-A, which are Nogo-A NiR- Δ2 (amino acids [aa] 59–172), NiG-Δ20 (aa 544–725) and Nogo-66 (aa 1019–1083), respectively. The diferent antigen recognition sites can be recognized by various antibody

Application: WB    Species: rat    Sample: neural stem cells

Fig. 1 Co-expression of Nogo-A-Δ20 and EphA4 in neural stem cells. a The monoclonal antibody (mAb) A563 against aa 563–627 (amino acids (aa) 563–627) of rat Nogo-A-NiG-Δ20, which is the main inhibitory region of rat Nogo-A. Immunofuorescence staining demonstrate that NSCs spheres are both positive for A563 and EphA4. Co- expression regions are labeled in yellow in the merged images. Staining of Hoechst 33,258 to show the nuclei. Scale bar 50 μm. b We dissociated and plated the neurospheres as single cells and performed Nogo-A and EphA4 double immunostaining. Colocalization are presented in yellow in the merged images. These were colocalization restricted to NSCs growing in the presence of growth factors. Scale bar 100 μm. c–f Western blot analysis detecting NogoA (~135  kDa), EphA4 (~110  kDa), GFAP (~50  kDa) and β-actin (~43 kDa) proteins in supernatants collected from lysis solutions of neurospheres. All bands presented are representative images of at three independent experiments. M marker. g The diagram represents the three inhibitory regions of rat Nogo-A, which are Nogo-A NiR- Δ2 (amino acids [aa] 59–172), NiG-Δ20 (aa 544–725) and Nogo-66 (aa 1019–1083), respectively. The diferent antigen recognition sites can be recognized by various antibody
2). Short-term high-fat diet favors the appearances of apoptosis and gliosis by activation of ERK1/2/p38MAPK pathways in brain. Imaging, 2021 (PubMed: 34620734) [IF=0.4]

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