DNMT3B Antibody - #AF5493

>>买2送1 已结束>>
>>赠JD卡 已结束>>
(6)   (10)  
产品: DNMT3B 抗体
货号: AF5493
描述: Rabbit polyclonal antibody to DNMT3B
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit
蛋白号: Q9UBC3
RRID: AB_2837973

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
DNMT3B Antibody detects endogenous levels of total DNMT3B.
RRID:
AB_2837973
引用格式: Affinity Biosciences Cat# AF5493, RRID:AB_2837973.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cytosine 5methyltransferase 3B; DNA; DNA (cytosine 5) methyltransferase 3 beta; DNA (cytosine 5)-methyltransferase 3B; DNA (cytosine-5)-methyltransferase 3B; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; DNM3B_HUMAN; Dnmt3b; EC 2.1.1.37; ICF; ICF1; M.HsaIIIB; MGC124407; RP23-89H14.3;

抗原和靶标

免疫原:

A synthesized peptide derived from human DNMT3B, corresponding to a region within N-terminal amino acids.

基因/基因ID:
DNMT3B(1789)
描述:
Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region.

研究领域

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Knockdown of DNMT1 Induces SLCO3A1 to Promote Follicular Growth by Enhancing the Proliferation of Granulosa Cells in Mammals. International journal of molecular sciences, 2024 (PubMed: 38473715) [IF=5.6]

Application: WB    Species: Mouse    Sample: GCs

Figure 5. Knockdown of DNMT1 upregulates the mRNA and protein levels of SLCO3A1. (A) RT-qPCR analysis of SLCO3A1 expression in the small (3 mm in diameter) follicles. (B) Western blot analysis of SLCO3A1 in the small and large follicles. (C) RT-qPCR analysis of SLCO3A1 expression in the DMSO or 5-Aza-CdR treated-cells. (D) Western blot analysis of SLCO3A1 in the DMSO or 5-Aza-CdR treated-cells. (E) Chromatin accessibility assay of region-1, region-2, and region-3 in the SLCO3A1 promoter treated with DMSO or 5-Aza-CdR (region-1, +971/+1252 bp, region-2, +1433/+1557 bp, and region-3, +1623/+1831 bp, transcription start site = +2000 bp). (F) RT-qPCR analysis of SLCO3A1 expression in GCs transfected with siRNAs of DNMT1, DNMT3A, or DNMT3B. Western blot analysis of SLCO3A1, DNMT1 (G), DNMT3A (H), and DNMT3B (I) in the siRNAs of DNMT1, DNMT3A, or DNMT3B treated-cells. *** p < 0.001.
2). Decreased ovarian function and autophagy gene methylation in aging rats. Journal of Ovarian Research, 2020 (PubMed: 32014030) [IF=4.0]

Application: WB    Species: rat    Sample: ovaries

Fig. 6| Protein expression of Dnmt1, Dnmt2, Dnmt3A and Dnmt3B in the ovaries of rats.(a) Western blot results.
3). Inhibitory effect of bound polyphenol from foxtail millet bran on miR-149 methylation increases the chemosensitivity of human colorectal cancer HCT-8/Fu cells. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2021 (PubMed: 33011952) [IF=3.5]

Application: WB    Species: human    Sample: HCT-8 and HCT-8/Fu cells

Fig. 4 |Efects of BPIS on the expression of DNA methylation-related enzymes. a, b The expressions of DNMT3a, DNMT3b and MECP2 were measured in RNA and protein levels in HCT-8 and HCT-8/Fu cells by RT-PCR and western blotting assays.
4). The mechanisms underlying olanzapine-induced insulin resistance via the brown adipose tissue and the therapy in rats. Adipocyte, 2022 (PubMed: 35067163) [IF=3.5]
5). Regulation of RUNX3 Expression by DNA Methylation in Prostate Cancer [Retraction]. Cancer Management and Research, 2021 (PubMed: 35002319) [IF=2.5]
6). Prenatal stress increases corticosterone levels in offspring by impairing placental glucocorticoid barrier function. Research Square, 2023

Application: WB    Species: Rat    Sample:

Figure 4. Effects of prenatal stress on offspring enzymes of DNA methylation in placenta. The figure showed that the expressions of DNMT1, DNMT 3A, DNMT 3B, and β-actin were identified by q-PCR and western blot. The levels of DNMT 3A and DNMT 3B were reduced but DNMT1 expression was enhanced by prenatal stress on offspring. Three biological replicates were carried out. DNMT1, DNA Methyltransferase 1; DNMT 3A, DNA Methyltransferase 3A; DNMT 3B, DNA Methyltransferase 3B. All data are shown as mean ± SD. Number of animals in each group = 3. *Statistically significant difference compared with the OPC group (P

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.