TSG6 Antibody - #AF5492

Fig. 7 MSCs-Exo enhanced TSG-6 expression in the colitis mice colon. a TSG-6 expression levels in colon tissue in the control, DSS + PBS, and DSS + Exo groups 10 days after colitis induction. b TSG-6 expression in the DSS + siNC Exo and DSS + siTSG-6 Exo groups. c TSG-6 protein expression in MSC-Exo. d The levels of TSG-6 in MSC-Exo and supernatant without exosomes. Exosomes in the supernatant were removed by ultrafiltration. All samples were adjusted to the same protein concentration, followed by ELISA. Data are presented as mean ± SD; n = 4 per group. *P < 0.05, **P < 0.01 and ns indicates P > 0.05

Figure 1 Representative photographs of SAH mice and the expression levels of TSG-6, NLRC4, GSDMD, and GSDMD-N after SAH. (A) Representative photographs of a sham and subarachnoid hemorrhage (SAH) mouse brain 24 hours after model construction. (B) Representative photographs of hematoxylin and eosin-stained slides of sham cerebral cortex and cortex 24 hours after SAH. Scale bar: 50 μm. (C, E) Western blot bands and quantification of TSG-6, NLRC4, GSDMD, and GSDMD-N proteins in sham mice and at 6, 12, 24, 48, and 72 hours after SAH. (D) Representative immunohistochemistry staining results for TSG-6 in the cortex of sham and SAH mice 24 hours after model construction. Scale bar: 50 μm. Values shown are means ± standard deviation of 6 samples from each group. **P < 0.01, ***P < 0.001, vs. sham group (one-way analysis of variance with Tukey’s honestly significant difference test). GSDMD: Gasdermin-D; NLRC4: NLR family CARD domain containing 4; SAH: subarachnoid hemorrhage; TSG-6: Tumor necrosis factor-stimulated gene-6; WB: western blot.

Figure 2 TSG-6 is expressed in astrocytes and neurons after subarachnoid hemorrhage (SAH). (A) Representative co-immunofluorescence (IF) images showing TSG-6 (green) in astrocytes (GFAP, red) of sham mice. (B) Representative co-IF images showing TSG-6 (green) in astrocytes (GFAP, red), microglia (Iba-1, red), and neurons (NeuN, red) of SAH mice 24 hours after SAH. Scale bars: 50 μm. (C, D) Quantification of IF staining results. Values shown are means ± standard deviation of 6 samples from each group. ****P < 0.0001 (one-way analysis of variance with Tukey’s honestly significant difference test [D] or Student’s t-test [C]). DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; Iba-1: ionized calcium-binding adapter molecule 1; IF: immunofluorescence; NeuN: neuronal nuclei antigen; SAH: subarachnoid hemorrhage; TSG-6: Tumor necrosis factor-stimulated gene-6.