TSG6 Antibody - #AF5492

Fig. 7 MSCs-Exo enhanced TSG-6 expression in the colitis mice colon. a TSG-6 expression levels in colon tissue in the control, DSS + PBS, and DSS + Exo groups 10 days after colitis induction. b TSG-6 expression in the DSS + siNC Exo and DSS + siTSG-6 Exo groups. c TSG-6 protein expression in MSC-Exo. d The levels of TSG-6 in MSC-Exo and supernatant without exosomes. Exosomes in the supernatant were removed by ultrafiltration. All samples were adjusted to the same protein concentration, followed by ELISA. Data are presented as mean ± SD; n = 4 per group. *P < 0.05, **P < 0.01 and ns indicates P > 0.05

Figure 1 Representative photographs of SAH mice and the expression levels of TSG-6, NLRC4, GSDMD, and GSDMD-N after SAH. (A) Representative photographs of a sham and subarachnoid hemorrhage (SAH) mouse brain 24 hours after model construction. (B) Representative photographs of hematoxylin and eosin-stained slides of sham cerebral cortex and cortex 24 hours after SAH. Scale bar: 50 μm. (C, E) Western blot bands and quantification of TSG-6, NLRC4, GSDMD, and GSDMD-N proteins in sham mice and at 6, 12, 24, 48, and 72 hours after SAH. (D) Representative immunohistochemistry staining results for TSG-6 in the cortex of sham and SAH mice 24 hours after model construction. Scale bar: 50 μm. Values shown are means ± standard deviation of 6 samples from each group. **P < 0.01, ***P < 0.001, vs. sham group (one-way analysis of variance with Tukey’s honestly significant difference test). GSDMD: Gasdermin-D; NLRC4: NLR family CARD domain containing 4; SAH: subarachnoid hemorrhage; TSG-6: Tumor necrosis factor-stimulated gene-6; WB: western blot.

Figure 2 TSG-6 is expressed in astrocytes and neurons after subarachnoid hemorrhage (SAH). (A) Representative co-immunofluorescence (IF) images showing TSG-6 (green) in astrocytes (GFAP, red) of sham mice. (B) Representative co-IF images showing TSG-6 (green) in astrocytes (GFAP, red), microglia (Iba-1, red), and neurons (NeuN, red) of SAH mice 24 hours after SAH. Scale bars: 50 μm. (C, D) Quantification of IF staining results. Values shown are means ± standard deviation of 6 samples from each group. ****P < 0.0001 (one-way analysis of variance with Tukey’s honestly significant difference test [D] or Student’s t-test [C]). DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; Iba-1: ionized calcium-binding adapter molecule 1; IF: immunofluorescence; NeuN: neuronal nuclei antigen; SAH: subarachnoid hemorrhage; TSG-6: Tumor necrosis factor-stimulated gene-6.

Figure 7 Validation of hub genes in vivo. (A–D) The mRNA expression level of AOC3, IRF4, TLR4 and TNFAIP6 in renal tissue of iohexol, cisplatin or vancomycin induced AKI mice compared with controls. (E–K) The protein expression level of AOC3, IRF4, TLR4 and TNFAIP6 in renal tissue of iohexol, cisplatin or vancomycin induced AKI mice compared with controls. *p

Figure 3. Overexpression of CIRBP improves YCC function. (A) Steroid-synthesis-related factors expression level after CIRBP overexpression, n = 4. (B) The mRNA expression level of cumulus-diffusion-related factors, n = 4. (C) The secretion of E2 and P4, n = 4. (D) E2 and P4 synthesis-related protein bands and analysis, n = 3. (E) Cumulus-diffusion-related protein bands and analysis, n = 3. (F) Immunofluorescence detection of cumulus-diffusion-related proteins. Values are mean ± SE, *: p < 0.05, **: p < 0.01, NS: no difference. Original Western blot images are provided in the Supplementary Materials.

Figure 3. Overexpression of CIRBP improves YCC function. (A) Steroid-synthesis-related factors expression level after CIRBP overexpression, n = 4. (B) The mRNA expression level of cumulus-diffusion-related factors, n = 4. (C) The secretion of E2 and P4, n = 4. (D) E2 and P4 synthesis-related protein bands and analysis, n = 3. (E) Cumulus-diffusion-related protein bands and analysis, n = 3. (F) Immunofluorescence detection of cumulus-diffusion-related proteins. Values are mean ± SE, *: p < 0.05, **: p < 0.01, NS: no difference. Original Western blot images are provided in the Supplementary Materials.

Fig. 7 Expression level of TNFAIP6 in Low- and High- CD133 expression groups (A): Representative IHC staining images of CD133 and TNFAIP6 in two stemness group in the Zhujiang in-house cohort. (B): Box plot displaying the pathology score levels of TNFAIP6 and CD133 based between two stemness group in the Zhujiang in-house dataset; (C): The correlation of expression level between TNFAIP6 and CD133.