VDAC1 Antibody - #AF5478

Fig. 2 Activation of VEGFR3 decreases renal oxidative stress levels and restored mitophagy in Ang II-induced hypertensive mice. A Significant statistics of GO term enrichment analysis (Control versus Ang II). B, C Renal tissues were stained to detect MitoSox Assay Kit (red). Scale bar, 100 μm. D Expressions of SQSTM1 and LC3 II/LC3 I were determined in mitochondria of the renal cortex from each group mice. E 3-nitrotyrosine, 8-oxo-dG, and SOD1 protein expression levels in renal cortex were examined by immunoblotting. F, G Immunoblots were statistically quantified by densitometry. H, I HE and Masson staining were carried out to assess the degree of renal tubular injury and fibrosis. J, K Semiquantitative scoring of tubular injury and renal fibrosis in different groups. Scale bar, 2–200 μm. n = 5 mice per group. Data in (B-K) were analyzed with one-way ANOVA. **P 

Figure 3. Effect of α-PA combined with 5-FU treatment on VDAC-1 and HK-2 protein expression in HT-29 cells. HT-29 cells (1.0×105 cells/30-mm plate) were treated with 50, 100 or 250 µg/mM α-PA combined with 5-FU for 72 h. (A) Immunocytochemical staining was performed to determine the expression levels of HK-2 (green) and VDAC-1 (red) (Hoechst 33342 (blue) (×400 magnification). Quantitative analysis of the relative expression levels of (B) HK-2 and (C) VDAC-1 after treatment. Data are presented as the mean ± SD (n=3-5). *P

Fig. 5. TRPV1 overexpression regulates the expression of mitophagy-related proteins in the cytoplasm and mitochondria in the TAC-induced HF model. (A) Representative Western blot images showing the protein expression of mitophagy markers (Parkin, PTEN-induced kinase 1 (PINK1), and sequestosome 1 (SQSTM1)) in the cytoplasm and mitochondria fractions. GAPDH and voltage-dependent anion channel 1 (VDAC1) were used as loading controls for the cytoplasm and mitochondria fractions, respectively. (B) Quantification of the cytoplasmic protein levels of Parkin, PINK1, and SQSTM1 in the experimental groups, with GAPDH as the standard. (C) Quantification of the mitochondrial protein levels of Parkin, PINK1, and SQSTM1 in the experimental groups, with VDAC1 as the standard. n = 8 per group. Data are presented as mean ± standard deviation.