HDAC10 Antibody - #AF5474

>>买2送1 进行中>>
>>赠JD卡 进行中>>
(1)   (7)  
产品: HDAC10 抗体
货号: AF5474
描述: Rabbit polyclonal antibody to HDAC10
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Dog
蛋白号: Q969S8
RRID: AB_2837956

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
HDAC10 Antibody detects endogenous levels of total HDAC10.
RRID:
AB_2837956
引用格式: Affinity Biosciences Cat# AF5474, RRID:AB_2837956.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZP761B039; HD 10; HD10; HDA10_HUMAN; HDAC 10; Hdac10; Histone deacetylase 10; MGC149722; OTTHUMP00000028555;

抗原和靶标

免疫原:

A synthesized peptide derived from human HDAC10, corresponding to a region within N-terminal amino acids.

基因/基因ID:
HDAC10(83933)
描述:
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.

研究领域

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

文献引用

1). Myeloid-specific Hdac10 deletion protects against LPS-induced acute lung injury via P62 acetylation at lysine 165. Respiratory research, 2024 (PubMed: 38956592) [IF=4.7]

Application: WB    Species: Mouse    Sample: lung tissues

Fig. 1 HDAC10 expression is increased in the lung macrophages following LPS exposure. A HDAC10 mRNA level in LPS-exposed ALI mice lung tissues. B, C HDAC10 protein level in LPS-stimulated ALI mice lung tissues. D, E Increased HDAC10 level in lung tissues of LPS-stimulated ALI mice measured by IHC staining for HDAC10. F, G IF staining of HDAC10 and F4/80 in mice lung tissues before or after LPS exposure. H, I WB analysis of HDAC10 in MH-S cells after 100 μg/ml LPS stimulation for 0-12 h and at different concentrations for 12 h. J, K HDAC10 mRNA expression in MH-S cells after 100 μg/ml LPS stimulation for 0-12 h and at different concentrations for 12 h. The data are expressed as the mean ±SEM (n = 3-5 mice/group). The data of Fig. 1G expressed as the median. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Data are representative of three independent experiments with similar results (A, E, J, K). See also Fig. S1

Application: IF/ICC    Species: Mouse    Sample: lung tissues

Fig. 1 HDAC10 expression is increased in the lung macrophages following LPS exposure. A HDAC10 mRNA level in LPS-exposed ALI mice lung tissues. B, C HDAC10 protein level in LPS-stimulated ALI mice lung tissues. D, E Increased HDAC10 level in lung tissues of LPS-stimulated ALI mice measured by IHC staining for HDAC10. F, G IF staining of HDAC10 and F4/80 in mice lung tissues before or after LPS exposure. H, I WB analysis of HDAC10 in MH-S cells after 100 μg/ml LPS stimulation for 0-12 h and at different concentrations for 12 h. J, K HDAC10 mRNA expression in MH-S cells after 100 μg/ml LPS stimulation for 0-12 h and at different concentrations for 12 h. The data are expressed as the mean ±SEM (n = 3-5 mice/group). The data of Fig. 1G expressed as the median. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Data are representative of three independent experiments with similar results (A, E, J, K). See also Fig. S1

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.