COX IV Antibody - #AF5468

Fig. 6 Levels of IRE1α-related pro-apoptotic protein caspase-3 and anti-apoptotic protein BCL-2. Expression of pro-apoptotic protein caspase-3 and anti-apoptotic protein BCL-2 (A, B) in CD4+ T cells. Protein expression were determined by western blotting and showed as the relative expression values of COX-IV. COX-IV was used as a loading control to normalize protein levels. Data are shown as Mean ± SD (n = 3). Statistically significant differences were determined by one-way analysis of variance (ANOVA) followed by Dunn’s/Tukey’s test. *P < 0.05, **P < 0.01, ****P < 0.0001

FIGURE 4 MiR‐130 inhibits hypoxia‐induced PASMC apoptosis by regulating PPARγ expression. (A–C) Western blotting for Bax, Bcl‐2, cleaved caspase‐3, caspase‐3 and apoptosis‐inducing factor (AIF) expression in PASMCs in Nor, Hyp, Hyp+miR‐130 inhibitor and Hyp+miR‐130 inhibitor+siPPARγ groups, β‐actin was used as a loading control (n = 4). (D‐F) The expression levels of cytochrome c (Cyt C) in mitochondrial and cytosol pellets in PASMCs were examined by western blotting with antibodies against Cyt C with COX IV as a mitochondria marker and β‐actin as the internal control (n = 4). (G) The apoptosis index of PASMCs in each group was measured by TUNEL assay (n = 10) (×200; scale bars indicate 100 µm) and is shown as the ratio of TUNEL positive cells (red) to total cells (blue). Data are presented as the mean ± SD. *p < 0.05, **p < 0.01

FIGURE 1. Zinc can inhibit Parthanatos in the spinal cord of SCI mice. (A) Western blot and (B) statistical results of western blots of PARP‐1 (n = 5, one‐way ANOVA followed by Bonferroni's post hoc test). (C) Immunofluorescence and (D) statistical analysis of immunofluorescence staining of PARP‐1 in nerve cells from each group (Scale bar = 50 μm, n = 3, one‐way ANOVA followed by Games–Howell post hoc test). (E) Mitochondrial membrane potential was measured with the JC‐1 probe (n = 5, one‐way ANOVA followed by Bonferroni's post hoc test). (F, H, J) Western blot and (G, I, K) statistical results of western blots of Mito‐AIF, Cyto‐AIF, and Nucleo‐AIF (n = 5, one‐way ANOVA followed by Bonferroni's post hoc test). The data represent means ± SD, * means, p 

Fig. 5 Effect of intervene in SIRT-1 and combine with OA on p62, Beclin-1, LC3B, SIRT-1, PGC-1a, MFN1, MFF and COX-IV protein expression in HepG2 cells. HepG2 cells were treated with 1.5 mM OA for 48 h, following T6 (2 μM) or CAY (20 μM) 2 h. a The p62, Beclin-1 and LC3B protein expression was detected by Western blotting. b The same method was used to detect the SIRT-1, PGC-1a, MFN1, MFF and COX-IV protein expression. The bands were normalized using GAPDH. The images were quantified with ImageJ. Data are presented as the mean ± SD; *P < 0.05, **P < 0.01 compared with CON group; #P < 0.05, ##P < 0.01 compared with OA group