HSP70 Antibody - #AF5466

Figure 4 BM-MSC transfer prevents post-stroke pneumonia by releasing migrasomes. A–C) BMDM were treated with GFP expressing E. Coli (E. Coli: BMDM = 20:1), and the clearance effect was evaluated at 1 h. (A) BMDM were pre-treated with the conditioned medium (CM) of BM-MSC overnight. Phagocytic efficiency of BMDM was assessed with immunostaining. Experiments were repeated three times. (B,C) BMDM were pre-treated with the solute or extracellular vesicles (EVs, 50 µg ml−1) of BM-MSC overnight. Phagocytic efficiency of BMDM to E. Coli-GFP was assessed with flow cytometry (B) and immunostaining (C). Experiments were repeated for three times. *p < 0.05, **p < 0.01, compared with PBS-treated group by one-way ANOVA (mean ± standard deviation). D) Ex vivo imaging of major organs from tMCAO mice at 2 h, 1d and 3d after intravenously injection of DiR-labeled BM-MSC (2 × 106 cells per mouse). n = 3 in each group. E–H) EVs were isolated from lung tissue of mice at 3d after tMCAO. (E) Schematic diagram of the EVs separation process. (F) Particle diameter of EVs isolated from the lung or blood of BM-MSC-treated stroke mice were quantified. n = 3 in each group. *p < 0.05, compared with blood by Student's t-test (mean ± standard deviation). (G) Transmission electron microscopy (TEM) analysis of the lung EVs isolated from BM-MSC-treated mice after negative staining. n = 3 in each group. (H) Expression of migrasome and exosome markers in lung EVs was analyzed with western blot. The experiments were repeated three times. I) Stroke mice were intravenously injected with WGA-labeled BM-MSC (red, 2×106 cells per mouse). Lung sections were collected at 2 h,1d and 3d after injection then subjected to immunostaining of Iba1 (green) to label macrophages and TSPAN4 (white) to label migrasome. n = 3 in each group. Representative images were displayed.

Figure 3. |(A) Fluorescence microscopy images of CRT exposure of different treated B16F10 cells. Nucleus: blue color, CRT: green color. The scale bars are 12.5 μm. (B) Western blots of HSP70 expression of different treated B16F10 cells (n = 3). Statistical significance: *p < 0.05, **p <0.01, and ***p < 0.001.

Figure 4.| MHO7 induced the release of DAMPs from TNBC cells.(B) Expression of CRT and HSP70 proteins in the cell cytosol and the cell membrane were measured by western blot under treatment with MHO7.

Figure 4.| Synergistic antitumor effects of an anti-PD-L1 mAb and ALA-PDT in a UV-induced cSCC mouse model. (g) After 6 h of treatment,A431 cell membrane lysates were subjected to Western blotting using the indicated antibodies. The blots were probed with Na-K-ATPase as the loading control