Collagen III Antibody - #AF5457

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产品: Collagen III 抗体
货号: AF5457
描述: Rabbit polyclonal antibody to Collagen III
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P02461
RRID: AB_2837941

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Collagen III Antibody detects endogenous levels of total Collagen III.
RRID:
AB_2837941
引用格式: Affinity Biosciences Cat# AF5457, RRID:AB_2837941.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Alpha 1 type III collagen; Alpha1 (III) collagen; CO3A1_HUMAN; COL 3A1; COL3A1; Collagen alpha 1(III) chain; Collagen alpha-1(III) chain; Collagen III alpha 1 chain precursor; Collagen III alpha 1 polypeptide; Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant); Collagen type III alpha 1; Collagen type III alpha 1 chain; Collagen type III alpha; Collagen, fetal; EDS4A; Ehlers Danlos syndrome type IV, autosomal dominant; Fetal collagen; Type III collagen;

抗原和靶标

免疫原:

A synthesized peptide derived from human Collagen III, corresponding to a region within N-terminal amino acids.

基因/基因ID:
COL3A1(1281)
描述:
Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.

研究领域

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Digestive system > Protein digestion and absorption.

文献引用

1). An asymmetric chitosan scaffold for tendon tissue engineering: In vitro and in vivo evaluation with rat tendon stem/progenitor cells. Acta Biomaterialia, 2018 (PubMed: 29678676) [IF=9.4]

Application: IF/ICC    Species: rat    Sample: tendon

Fig. 6. | Analyses of tendon tissue by immunofluorescence. Fluorescence immunohistochemistry at the regenerated tendon in each group at 4 weeks. (A-D) The production of COL1; (E-H) The production of COL3; (I-L) The production of TNMD; (M-P) The production of CD31. Blue: DAPI (cell nuclei); Green:COL1/COL3/CD31; Red: TNMD. Scale bar = 100 μm.

Application: IHC    Species: rat    Sample: tendon

Fig. 5.| Detailed analyses of tendon tissue organization and immunohistochemistry. (A-E) DAPI staining and chart of calculated cell density. Data were mean ± S.D. * p < 0.05 vs. ED group. (F-Q)Representative images of regenerated tendon in each group at 4 weeks. Scale bar =100 μm.
2). A polysaccharide from Glycyrrhiza uralensis attenuates myocardial fibrosis via modulating the MAPK/PI3K/AKT signaling pathway. International journal of biological macromolecules, 2025 (PubMed: 39617235) [IF=7.7]
3). Intratunical injection of rat-derived bone marrow mesenchymal stem cells prevents fibrosis and is associated with increased Smad7 expression in a rat model of Peyronie's disease. Stem cell research & therapy, 2022 (PubMed: 35908015) [IF=7.5]

Application: IHC    Species: Rat    Sample:

Fig. 3BMSCs injection prevented collagen 3 expression and promoted Smad7 expression in the TA of the rats with PD. The positive expression levels of collagen 3 A and Smad7 B were analysed by immunohistochemistry. The positive expression levels were analysed using ImageJ software. *P 

Application: WB    Species: Rat    Sample:

Fig. 5 The expression levels of Smad7, collagen 3, elastase-2B and osteopontin in the TA of the rats with PD were analysed by western blot (A). The full-length gels were included in Additional file 1. The relative expression levels of Smad7 (B), collagen 3 (C), elastase-2B D and osteopontin E were analysed by ImageJ software. *P 
4). Ginsenoside RH4 inhibits Ang II-induced myocardial remodeling by interfering with NFIL3. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38359490) [IF=6.9]
5). Fisetin treatment alleviates kidney injury in mice with diabetes-exacerbated atherosclerosis through inhibiting CD36/fibrosis pathway. Acta Pharmacologica Sinica, 2023 (PubMed: 37225845) [IF=6.9]
6). Modified citrus pectin ameliorates myocardial fibrosis and inflammation via suppressing galectin-3 and TLR4/MyD88/NF-κB signaling pathway. Biomedicine & Pharmacotherapy, 2020 (PubMed: 32172066) [IF=6.9]

Application: IHC    Species: rat    Sample: heart

Fig. 5. |Effects of MCP on ISO-induced myocardial fibrosis in rats. (A and B) Immunohistochemical analysis of collagen I and collagen Ⅲ protein in heart cross sections of different groups on day 15 and day 22 (200× magnification). Scale bars 100 μm.

Application: WB    Species: rat    Sample: heart

Fig. 6. |Effects of MCP on the expression of collagen Ⅰ and collagen Ⅲ induced by ISO in rats. Protein and mRNA levels of collagen Ⅰ and collagen Ⅲ were analyzed by western blot and qRT-PCR in each group on day 15 and day 22. (A) Protein expression of collagen Ⅰ and collagen Ⅲ were measured by western blot.
7). Protective role of Gentianella acuta on isoprenaline induced myocardial fibrosis in rats via inhibition of NF-κB pathway. BIOMEDICINE & PHARMACOTHERAPY, 2019 (PubMed: 30554111) [IF=6.9]
8). Multi-omic analysis revealed the therapeutic mechanisms of Alpinia oxyphylla fructus water extract against bladder overactivity in spontaneously hypertensive rats. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 37976696) [IF=6.7]
9). Particulate Matter Induces Activation of Cardiac Fibroblasts via HIF1α-Mediated Autophagy. Environmental Sciences Europe, 2025 [IF=6.0]

Application: IF/ICC    Species: Rat    Sample:

Fig. 2 PM triggered fibrosis in NRCFs. a The procedures of cardiac fibroblasts extraction from neonatal rats. b The cell viability of NRCFs after exposure of PM (n = 6). c The representative images of immunofluorescence staining and the fluorescence intensity of α-SMA, CTGF, COL1A1, COL3A1 in NRCFs after exposure of PM for different time periods (n = 3). d Fibrosis-related genes expressions after PM exposure (n = 3). ns, not significant; *P 
10). Daucosterol Alleviates Alcohol−Induced Hepatic Injury and Inflammation through P38/NF−κB/NLRP3 Inflammasome Pathway. Nutrients, 2023 (PubMed: 36615880) [IF=5.9]
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