产品: 磷酸化 ATRIP (Ser224) 抗体
货号: AF5437
描述: Rabbit polyclonal antibody to Phospho-ATRIP (Ser224)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Bovine, Sheep, Rabbit
分子量: 85 kDa; 86kD(Calculated).
蛋白号: Q8WXE1
RRID: AB_2837921

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Sheep(86%), Rabbit(88%)
克隆:
Polyclonal
特异性:
Phospho-ATRIP (Ser224) Antibody detects endogenous levels of ATRIP only when phosphorylated at Ser224.
RRID:
AB_2837921
引用格式: Affinity Biosciences Cat# AF5437, RRID:AB_2837921.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AGS1; ATIP; ATM and Rad3 related interacting protein; ATM and Rad3-related-interacting protein; ATR interacting protein; ATR-interacting protein; Atrip; ATRIP_HUMAN; DKFZp762J2115; FLJ12343; MGC20625; MGC21482; MGC26740;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q8WXE1 ATRIP_HUMAN:

Ubiquitous.

描述:
Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
序列:
MAGTSAPGSKRRSEPPAPRPGPPPGTGHPPSKRARGFSAAAAPDPDDPFGAHGDFTADDLEELDTLASQALSQCPAAARDVSSDHKVHRLLDGMSKNPSGKNRETVPIKDNFELEVLQAQYKELKEKMKVMEEEVLIKNGEIKILRDSLHQTESVLEEQRRSHFLLEQEKTQALSDKEKEFSKKLQSLQSELQFKDAEMNELRTKLQTSERANKLAAPSVSHVSPRKNPSVVIKPEACSPQFGKTSFPTKESFSANMSLPHPCQTESGYKPLVGREDSKPHSLRGDSIKQEEAQKSFVDSWRQRSNTQGSILINLLLKQPLIPGSSLSLCHLLSSSSESPAGTPLQPPGFGSTLAGMSGLRTTGSYDGSFSLSALREAQNLAFTGLNLVARNECSRDGDPAEGGRRAFPLCQLPGAVHFLPLVQFFIGLHCQALQDLAAAKRSGAPGDSPTHSSCVSSGVETNPEDSVCILEGFSVTALSILQHLVCHSGAVVSLLLSGVGADSAAGEGNRSLVHRLSDGDMTSALRGVADDQGQHPLLKMLLHLLAFSSAATGHLQASVLTQCLKVLVKLAENTSCDFLPRFQCVFQVLPKCLSPETPLPSVLLAVELLSLLADHDQLAPQLCSHSEGCLLLLLYMYITSRPDRVALETQWLQLEQEVVWLLAKLGVQSPLPPVTGSNCQCNVEVVRALTVMLHRQWLTVRRAGGPPRTDQQRRTVRCLRDTVLLLHGLSQKDKLFMMHCVEVLHQFDQVMPGVSMLIRGLPDVTDCEEAALDDLCAAETDVEDPEVECG

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Bovine
100
Rabbit
88
Sheep
86
Pig
75
Dog
63
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q8WXE1 作为底物

Site PTM Type Enzyme
S9 Phosphorylation
S13 Phosphorylation
T26 Phosphorylation
S31 Phosphorylation
K32 Acetylation
S38 Phosphorylation
S68 Phosphorylation Q13535 (ATR)
S72 Phosphorylation Q13535 (ATR)
K96 Ubiquitination
K122 Ubiquitination
S162 Phosphorylation
K205 Ubiquitination
K214 Ubiquitination
S219 Phosphorylation
S221 Phosphorylation
S224 Phosphorylation P24941 (CDK2)
S239 Phosphorylation P24941 (CDK2)
K270 Ubiquitination
K295 Ubiquitination
S305 Phosphorylation
T307 Phosphorylation
S310 Phosphorylation
S512 Phosphorylation
S518 Phosphorylation
T523 Phosphorylation
Y636 Phosphorylation

研究背景

功能:

Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.

翻译修饰:

Phosphorylated by ATR.

细胞定位:

Nucleus.
Note: Redistributes to discrete nuclear foci upon DNA damage.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous.

亚基结构:

Interacts with ATR (By similarity). Heterodimer with ATR. The heterodimer binds the RPA complex and is then recruited to single-stranded DNA. Interacts with CEP164 (via N-terminus). Interacts with CINP.

蛋白家族:

The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.

Belongs to the ATRIP family.

研究领域

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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