TERT Antibody - #AF5413

Figure 1 Human telomerase reverse transcriptase (hTERT) promotes the invasion of gastric cancer cells by upregulating integrin β1 (ITGB1). (A) Western blot analysis of hTERT in U2OS, SGC-7901 and HGC-27 cells. The cells were infected with lenti-hTERT and the corresponding control lentivirus. (B) Transwell analysis of the effects of hTERT on the invasion of the cancer cells. After 48 h of incubation on the top of the Transwell insert, the cells were fixed and stained, and the number of invasion cells was calculated. (C) Ten candidate molecules with more than fivefold (log ratio) increases in hTERT-overexpressed U2OS cells, revealed by proteomics analysis. (D) Western blot analysis of the expression of the above candidates in hTERT-overexpressed cancer cells and corresponding control cells. (E) Western blot analysis of knockdown of ITGB1 after hTERT overexpression. Stable SGC-7901 and HGC-27 cells were transfected with siRNA targeting ITGB1. Forty-eight hours later, the cells were harvested for western blot analysis. (F) ITGB1-mediated cell adhesion assay after knockdown of ITGB1. Cells were treated as in (E) in six-well plates, and then adhesion assays were performed. (G) Cell invasion assays after knockdown of ITGB1. Six hours after the transfection of siRNAs against ITGB1 in the stable cells, SGC-7901 and HGC-27 cells were resuspended and seeded in Transwells for another 48 h, and the number of invasion cells was calculated. *p<0.05, **p<0.01.

Figure 2 Human telomerase reverse transcriptase (hTERT) expression parallels that of integrin β1 (ITGB1) in gastric cancer tissues, and both are correlated with poor prognosis. (A) Representative immunohistochemical staining of hTERT and ITGB1 in gastric cancer tissues and corresponding adjacent non-cancerous tissues. (B) The association between hTERT/ITGB1 expression and TNM stage/lymphatic metastasis in patients with gastric cancer (p<0.01). (C) The correlations between the protein levels of hTERT and ITGB1 (p<0.01). (D) The receiver operating characteristic (ROC) curves for predicting patients’ survival time using hTERT or ITGB1 expression. (E) Kaplan–Meier analyses of overall survival according to hTERT or ITGB1 expression levels (p<0.01). (F) Kaplan–Meier analyses of overall survival according to the combination of the above two indices (p<0.01). AUC, area under the curve; TNM, tumour node metastases.