BMPR2 Antibody - #AF5383

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产品: BMPR2 抗体
货号: AF5383
描述: Rabbit polyclonal antibody to BMPR2
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat, Monkey
预测: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 115 kDa; 115kD(Calculated).
蛋白号: Q13873
RRID: AB_2837868

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
预测:
Bovine(100%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
BMPR2 Antibody detects endogenous levels of total BMPR2.
RRID:
AB_2837868
引用格式: Affinity Biosciences Cat# AF5383, RRID:AB_2837868.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BMP type II receptor; BMP type-2 receptor; BMPR 2; BMPR 3; BMPR II; BMPR-2; BMPR-II; Bmpr2; BMPR2_HUMAN; BMPR3; BMPRII; BMR 2; BMR2; Bone morphogenetic protein receptor type 2; Bone morphogenetic protein receptor type II; Bone morphogenetic protein receptor type-2; Bone morphogenic protein receptor type II serine threonine kinase; BRK 3; BRK3; PPH 1; PPH1; Serine threonine kinase type II activin receptor like kinase; T ALK; TALK; Type II activin receptor like kinase;

抗原和靶标

免疫原:
Uniprot:

Q13873(BMPR2_HUMAN) >>Visit HPA database.

基因/基因ID:
BMPR2(659)
表达:
Q13873 BMPR2_HUMAN:

Highly expressed in heart and liver.

描述:
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators.
序列:
MTSSLQRPWRVPWLPWTILLVSTAAASQNQERLCAFKDPYQQDLGIGESRISHENGTILCSKGSTCYGLWEKSKGDINLVKQGCWSHIGDPQECHYEECVVTTTPPSIQNGTYRFCCCSTDLCNVNFTENFPPPDTTPLSPPHSFNRDETIIIALASVSVLAVLIVALCFGYRMLTGDRKQGLHSMNMMEAAASEPSLDLDNLKLLELIGRGRYGAVYKGSLDERPVAVKVFSFANRQNFINEKNIYRVPLMEHDNIARFIVGDERVTADGRMEYLLVMEYYPNGSLCKYLSLHTSDWVSSCRLAHSVTRGLAYLHTELPRGDHYKPAISHRDLNSRNVLVKNDGTCVISDFGLSMRLTGNRLVRPGEEDNAAISEVGTIRYMAPEVLEGAVNLRDCESALKQVDMYALGLIYWEIFMRCTDLFPGESVPEYQMAFQTEVGNHPTFEDMQVLVSREKQRPKFPEAWKENSLAVRSLKETIEDCWDQDAEARLTAQCAEERMAELMMIWERNKSVSPTVNPMSTAMQNERNLSHNRRVPKIGPYPDYSSSSYIEDSIHHTDSIVKNISSEHSMSSTPLTIGEKNRNSINYERQQAQARIPSPETSVTSLSTNTTTTNTTGLTPSTGMTTISEMPYPDETNLHTTNVAQSIGPTPVCLQLTEEDLETNKLDPKEVDKNLKESSDENLMEHSLKQFSGPDPLSSTSSSLLYPLIKLAVEATGQQDFTQTANGQACLIPDVLPTQIYPLPKQQNLPKRPTSLPLNTKNSTKEPRLKFGSKHKSNLKQVETGVAKMNTINAAEPHVVTVTMNGVAGRNHSVNSHAATTQYANGTVLSGQTTNIVTHRAQEMLQNQFIGEDTRLNINSSPDEHEPLLRREQQAGHDEGVLDRLVDRRERPLEGGRTNSNNNNSNPCSEQDVLAQGVPSTAADPGPSKPRRAQRPNSLDLSATNVLDGSSIQIGESTQDGKSGSGEKIKKRVKTPYSLKRWRPSTWVISTESLDCEVNNNGSNRAVHSKSSTAVYLAEGGTATTMVSKDIGMNCL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Rabbit
100
Dog
92
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Binds to BMP7, BMP2 and, less efficiently, BMP4. Binding is weak but enhanced by the presence of type I receptors for BMPs. Mediates induction of adipogenesis by GDF6.

细胞定位:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in heart and liver.

亚基结构:

Interacts with GDF5.

蛋白家族:

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Development > Axon guidance.   (View pathway)

文献引用

1). Exosomal miR-100-5p inhibits osteogenesis of BMSCs and angiogenesis of VECs by suppressing BMPR2/Smad1/5/9 signaling pathway. Stem Cell Research & Therapy, 2021 (PubMed: 34256859) [IF=7.5]

Application: WB    Species: Human    Sample: HUVECs

Fig. 6 MiR-100-5p inhibited osteogenesis of hBMSCs and angiogenesis of HUVECs, promoting adipogenesis of hBMSCs. A RT-PCR was used to detect the expression of miR-100-5p in hBMSCs. B WB was used to detect the expression of BMPR2, collagen type 1, OCN, RUNX2, ALP, OPN, and PPARγ in hBMSCs. C The expression of miR-100-5p in HUVECs. D WB was used to measure the expression of BMPR2, FGF2, and VEGFA in HUVECs. E ALP staining of hBMSCs (scale bar = 100 μm). F Alizarin Red S staining of hBMSCs after cultured in ODM for 14 days. G Oil Red O staining of hBMSCs (scale bar = 50 μm). H Tube formation assay of HUVECs (scale bar = 200 μm). NM-CTL, controls for differentiation. *P < 0.05, versus NC group; #P < 0.05, versus agomiR-100-5p group. All data were expressed as mean ± SEM
2). Magnesium-enriched microenvironment promotes odontogenic differentiation in human dental pulp stem cells by activating ERK/BMP2/Smads signaling. Stem Cell Research & Therapy, 2019 (PubMed: 31823825) [IF=7.5]

Application: WB    Species: Human    Sample: DPSCs

Fig. 6 The ERK and BMP2 signaling pathway is activated by high extracellular Mg2+ in DPSCs during odontogenic differentiation. a ERK phosphorylation was significantly enhanced in DPSCs treated with 1 mM, 5 mM, and 10 mM Mg2+ compared with the 0 mM Mg2+ group, but p38 and JNK phosphorylation amounts were unchanged. b ERK phosphorylation was reduced by 2-APB. c Consistently, the protein levels of BMP2, BMPR1, and phosphorylated Smad1/5/9 were significantly increased in DPSCs exposed to high extracellular Mg2+. d BMP2, BMPR1, and phosphorylated Smad1/5/9 protein amounts were decreased by 2-APB
3). RGMa Participates in the Blood–Brain Barrier Dysfunction Through BMP/BMPR/YAP Signaling in Multiple Sclerosis. Frontiers in Immunology, 2022 (PubMed: 35664003) [IF=5.7]

Application: WB    Species: Mice    Sample: brain and spinal cord

Figure 3 BMP2 and BMPR II expression in EAE mice. (A–F) BMP2 and BMPR II in the brain and spinal cord were both upregulated in the 14- and 21-day group compared with the control group. BMP2 and BMPR II in both the brain and spinal cord were significantly higher in the 21-day than the 0-day groups (n = 4, error bar: SEM; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one-way ANOVA with Bonferroni). (G–J) Immunofluorescence showed BMP2 and BMPR II were located in CD31+ endothelial cells in both the brain cortex and the white matter of the spinal cord. Scale bar: 50 µm (brain), 25 µm (spinal cord).
4). Activation of the BMP2-SMAD1-CGRP pathway in dorsal root ganglia contributes to bone cancer pain in a rat model. Heliyon, 2024 (PubMed: 38496903) [IF=4.0]

Application: WB    Species: Rat    Sample:

Fig. 4. BMPRs are involved in BCP. (A) Western blot analysis of BMPR1B levels in the L3-L5 DRG ipsilateral to the tumor-bearing bone in rats with BCP versus sham controls on day 21. n = 8 per group. ***p = 0.0002 (Mann Whitney test). Top: representative Western blot. Bottom: quantification of BMPR1B expression levels. (B) Western blot analysis of BMPR2 levels in rats with BCP versus sham control rats. n = 8 per group. *p = 0.0281 (Mann Whitney test). Top: representative Western blot. Bottom: quantification of BMPR2 expression levels. (C) Paw withdrawal mechanical threshold (PWMT) in BCP animals given LDN193189 or vehicle (****p < 0.0001); two-way ANOVA with the Geisser-Greenhouse correction and Tukey's multiple comparisons test. (D) Limb use scores in BCP animals given LDN193189 or vehicle (*p = 0.0168, **p = 0.0062, ***p = 0.0005 and 0.0004 for day 11 and day 14 respectively, ****p < 0.0001); two-way ANOVA with the Geisser-Greenhouse correction and Tukey's multiple comparisons test.

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