Cleaved-Caspase 4 (Gln81) Antibody - #AF5373

Figure 9. GLI2 promoted drug tolerance. (A) Expression level of GLI2 after siRNA treatment in colorectal cancer cell (lovo) and gastric cancer by (a) PCR assay and (B) WB. (C) CCK-8 assay. Alive and dead cell staining in (D) colorectal cancer and (E) gastric cancer.

Fig. 4 F1/F3 mediates HeLa cell pyroptosis through the caspase-3/GSDME signalling pathway. (A)-(I): HeLa cells (1.0 × 106) were treated with F1/F3 (10 µg/ml), P3 or left untreated for 1 h, the expression of pyroptosis-related proteins was tested by Western blot. (A) GSDMD. (B) Caspase-1. (C) Caspase-4. (D) Caspase-5. (E) Cleaved caspase-4. (F) GSDMB. (G) Caspase-3. (H) Cleaved caspase-3. (I) Cleaved GSDME-N. (J) LDH release was detected by F1/F3, caspase inhibitor and caspase-3 inhibitor (Ac-DEVD-CHO) combined with F1/F3. These results come from a representative experiment in which two independent experiments were conducted. All data are presented as the mean ± SEM. *P-value 

Figure 4. Melatonin attenuates DON-induced apoptosis by suppressing ER stress in human granulosa cells. (A) Cells were treated with different concentrations of DON for 24 h. The protein levels of BiP, CHOP, and phosphorylated-eIF2α were assessed by western blot. (B) For the inhibition of ROS production, Mito-TEMPO was added 1 h before DON treatment. Cells were cultured for another 24 h and the levels of BiP, CHOP, and phosphorylated-eIF2α were measured by western blot. (C) Granulosa cells were cultured in medium containing DON with or without melatonin for 24 h. The levels of BiP, CHOP, and phosphorylated-eIF2α were analyzed by western blot. (D) The levels of Cleaved Caspase 4 and Phosphorylated JNK were analyzed by western blot. (E) ER stress inhibitor 4-PBA was added 1 h before DON and melatonin incubation. The cells were cultured for 24 h and cell viability was determined using MTT assay kit. (F) The protein levels of Bax, Bcl-2, and Cleaved Caspase-3 from granulosa cells treated as depicted in Figure 4D were analyzed by western blot. (G) Quantification of Bcl-2 and Bax expression using densitometric analysis. β-actin served as the control for loading. Data are represented as means ± SEM of three independent experiments. ∗P < 0.05, ∗∗P < 0.01.