AurB Antibody - #AF5361

Fig. 8. Blocking Piezo1/Ca2+ signaling could inhibit the expression of AURKB and counteract senescence under mechanical strain (a) Visualization of Ca2+ through Flou-4 am fluorescent probe and quantitative analysis (n = 3). (b and c) RT-qPCR analysis of AURKB, P16, P21, LaminB1, HP1γ and H3K9me3 in chondrocytes treated with si-Piezo1 and BAPTA under mechanical strain (n = 3). (d) Western blots analysis (d) and relative protein expression (e, f, and g) of AURKB, P16, P21, LaminB1, COL2A1, SOX9, KAP1, HP1γ, and H3K9me3 in chondrocytes treated with si-Piezo1 and BAPTA under mechanical strain (n = 3). (h) Representative immunofluorescence images of HP1γ, H3K9me3, and KAP1 in chondrocytes treated with si-Piezo1 and BAPTA under mechanical strain. Scale bar: 50 µm. * , p 

Fig. 2. AURKB was upregulated in OA chondrocytes and mechanical strain treated chondrocytes. (a) Representative images of HE, Safranin-O/Fast Green staining of Sham and OA rat cartilage, OARSI score was used to quantify the degree of OA(n = 6). (b) Immunohistochemistry staining of Sham and OA rat cartilage, and the percentage of AURKB positive cells (n = 6). (c) Schematic diagram of the mechanical strain treatment on chondrocytes. (d) RT-qPCR analysis of Piezo1 in chondrocytes exposed to mechanical strain (n = 5). (e) The protein level of AURKB in chondrocytes was determined by western blots (n = 3). (f) Representative immunofluorescence images of AURKB in chondrocytes and mean fluorescence intensity (n = 3). * , p