HDAC5 Antibody - #AF5348

Fig. 2. AKAP5-mediated cardiomyocyte hypertrophy CaMKII/HDAC pathway. (A) Expression of CaMKII/HDAC and hypertrophy-related proteins after H/R and AKAP5 transfection; (B) Expression of CaMKII/HDAC proteins after H/R and siAKAP5 transfection and KN93(10 uM, 1 h) intervention. Analysis by one-way ANOVA, All data are presented as the means ± SD (n = 3). ***P < 0.001 and ****P < 0.0001 vs. the CON group; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. the H/R group; **P < 0.01, ***P < 0.001 and ****P < 0.0001 vs. the H/R + siAKAP5 group. CON, normal control group; H/R, hypoxia-reoxygenation group; NC, empty vector group; siAKAP5, siRNA-AKAP5 transfection group; KN93, an inhibitor of calmodulin-dependent kinase type II; AKAP5, A-kinase anchoring protein 5; CaMKII, Ca2+/CaM-dependent protein kinase II; HDAC4 /5, Histone deacetylases 4/5; MEF2C, Myocyte enhancer factor 2C.

Fig. 4. |Histone deacetylase 5 (HDAC5) is involved in WNT10A-induced apoptosis of Sn-SMSCs: B: Immunohistochemistry staining for HDAC5 (scale bars = 50 μm) and caspase3 (scale bars = 100 μm) after 0/1/24/72 h of culture in complete medium supplemented with 50 ng/mL WNT10A(n = 5).

Fig. 4. |Histone deacetylase 5 (HDAC5) is involved in WNT10A-induced apoptosis of Sn-SMSCs: C: HDAC5 mRNA (left), protein (middle) levels. and HDAC5 phosphorylation at s498 (right); *P<0.05

Fig. 4. Histone deacetylase 5 (HDAC5) is involved in WNT10A-induced apoptosis of Sn-SMSCs: A: RNA-seq results after 3 days of culture in complete medium supplemented with PBS (control) or 50 ng/mL WNT10A (n = 3). A: Top 10 hub proteins (left) and PPI (right). B: Immunohistochemistry staining for HDAC5 (scale bars = 50 μ m) and caspase3 (scale bars = 100 μ m) after 0/1/ 24/72 h of culture in complete medium supplemented with 50 ng/mL WNT10A (n = 5). C: HDAC5 mRNA (left), protein (middle) levels. and HDAC5 phos- phorylation at s498 (right); *P<0.05.

Fig. 4. Histone deacetylase 5 (HDAC5) is involved in WNT10A-induced apoptosis of Sn-SMSCs: A: RNA-seq results after 3 days of culture in complete medium supplemented with PBS (control) or 50 ng/mL WNT10A (n = 3). A: Top 10 hub proteins (left) and PPI (right). B: Immunohistochemistry staining for HDAC5 (scale bars = 50 μ m) and caspase3 (scale bars = 100 μ m) after 0/1/ 24/72 h of culture in complete medium supplemented with 50 ng/mL WNT10A (n = 5). C: HDAC5 mRNA (left), protein (middle) levels. and HDAC5 phos- phorylation at s498 (right); *P<0.05.

Fig. 5. WNT10A phosphorylates HDAC5 via the Wnt/calcium pathway: A: Expression of β-catenin and GSK3β (left, upper); expression of p-JNK and JNK (left, lower); expression of p-PKC and PKC, p-CaMKII and CaMKII, p-NFAT, and NFAT (middle); ratio of p-PKC:PKC, p-CaMKII:CaMKII and p-NFAT:NFAT (right) after 24 h of culture in complete medium supplemented with PBS (control) or 50 ng/mL WNT10A. B: Measurement of intracellular Ca2+ concentration using Fluo-4 AM. C: Expression of p-CaMKII and CaMKII, p-HDAC5 s498 and HDAC5, and p53 and acetylation p53 at K120 (upper); ratio of p-CaMKII:CaMKII and p-HDAC5 s498:HDAC5 (lower) after 24 h of culture in complete medium supplemented with PBS (control), 50 ng/mL WNT10A, or 50 ng/mL WNT10A + 10 μ M KN-62 (an inhibitor of CaMKII) (n = 5). D: Immunofluorescence staining for HDAC5 after 24 h of culture in complete medium supplemented with PBS (control), 50 ng/mL WNT10A, or 50 ng/mL WNT10A + 10 μ M KN-62 (n = 5); scale bars = 50 μ m; *P<0.05, **P<0.01.