FRA2 Antibody - #AF5345

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产品: FRA2 抗体
货号: AF5345
描述: Rabbit polyclonal antibody to FRA2
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 35-45kD; 35kD(Calculated).
蛋白号: P15408
RRID: AB_2837830

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
FRA2 Antibody detects endogenous levels of total FRA2.
RRID:
AB_2837830
引用格式: Affinity Biosciences Cat# AF5345, RRID:AB_2837830.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ23306; Fos L2; FOS like antigen 2; Fos related antigen 2; Fos-related antigen 2; FosL 2; Fosl2; FOSL2_HUMAN; FRA 2; FRA-2;

抗原和靶标

免疫原:
Uniprot:

P15408(FOSL2_HUMAN) >>Visit HPA database.

基因/基因ID:
FOSL2(2355)
描述:
Controls osteoclast survival and size (By similarity). As a dimer with JUN, activates LIF transcription (By similarity). Activates CEBPB transcription in PGE2-activated osteoblasts.
序列:
MYQDYPGNFDTSSRGSSGSPAHAESYSSGGGGQQKFRVDMPGSGSAFIPTINAITTSQDLQWMVQPTVITSMSNPYPRSHPYSPLPGLASVPGHMALPRPGVIKTIGTTVGRRRRDEQLSPEEEEKRRIRRERNKLAAAKCRNRRRELTEKLQAETEELEEEKSGLQKEIAELQKEKEKLEFMLVAHGPVCKISPEERRSPPAPGLQPMRSGGGSVGAVVVKQEPLEEDSPSSSSAGLDKAQRSVIKPISIAGGFYGEEPLHTPIVVTSTPAVTPGTSNLVFTYPSVLEQESPASPSESCSKAHRRSSSSGDQSSDSLNSPTLLAL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Controls osteoclast survival and size. As a dimer with JUN, activates LIF transcription. Activates CEBPB transcription in PGE2-activated osteoblasts.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterodimer.

蛋白家族:

Belongs to the bZIP family. Fos subfamily.

研究领域

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

文献引用

1). FOSL2 participates in renal fibrosis via SGK1-mediated epithelial-mesenchymal transition of proximal tubular epithelial cells. Journal of Translational Internal Medicine, 2023 (PubMed: 37662889) [IF=4.7]

Application: WB    Species: Mouse    Sample: kidneys

Figure 2 The expression level of FOSL2 is elevated in UUO-injured kidneys. (A) Schematic diagram of UUO and adenovirus infection experiments in mice. (B) Western blotting analyses of protein expression levels of α-SMA, fibronectin, and collagen type I at days 3, 7, and 14. (C) Immunohistochemistry was conducted with anti-FOSL2 antibody to demonstrate FOSL2 protein expression in renal tissues. Magnification: 400×. Scale bar: 50 μm. (D) The mRNA and protein expression levels of FOSL2 were analyzed using qRT-PCR and western blotting, respectively. The values are expressed as mean ± standard deviation. *P < 0.05 versus sham (Day 3) group; ##P < 0.01 versus sham (Day 7) group, ++P < 0.01 versus sham (Day 14) group. UUO: unilateral ureteral obstruction; α-SMA: α-smooth muscle actin; qRT-PCR: quantitative real-time polymerase chain reaction; FOSL2: fos-related antigen 2.

Application: IHC    Species: Mouse    Sample: kidneys

Figure 2 The expression level of FOSL2 is elevated in UUO-injured kidneys. (A) Schematic diagram of UUO and adenovirus infection experiments in mice. (B) Western blotting analyses of protein expression levels of α-SMA, fibronectin, and collagen type I at days 3, 7, and 14. (C) Immunohistochemistry was conducted with anti-FOSL2 antibody to demonstrate FOSL2 protein expression in renal tissues. Magnification: 400×. Scale bar: 50 μm. (D) The mRNA and protein expression levels of FOSL2 were analyzed using qRT-PCR and western blotting, respectively. The values are expressed as mean ± standard deviation. *P < 0.05 versus sham (Day 3) group; ##P < 0.01 versus sham (Day 7) group, ++P < 0.01 versus sham (Day 14) group. UUO: unilateral ureteral obstruction; α-SMA: α-smooth muscle actin; qRT-PCR: quantitative real-time polymerase chain reaction; FOSL2: fos-related antigen 2.
2). Circ_0005615 Regulates the Progression of Colorectal Cancer Through the miR-873-5p/FOSL2 Signaling Pathway. Biochemical genetics, 2023 (PubMed: 36920708) [IF=2.1]

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