(15)
(20)
- WB
- IHC
- IF/ICC
- Cites
1/20
FAP Antibody - AF5344 at 1/100 staining Human normal tissues adjacent to esophageal cancer by IHC-P.
FAP Antibody - AF5344 at 1/100 staining Human normal tissues adjacent to colorectal cancer by IHC-P.
产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
FAP1 Antibody detects endogenous levels of total FAP.
RRID:
AB_2837829
引用格式: Affinity Biosciences Cat# AF5344, RRID:AB_2837829.
引用格式: Affinity Biosciences Cat# AF5344, RRID:AB_2837829.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
170 kDa melanoma membrane bound gelatinase; 170 kDa melanoma membrane-bound gelatinase; DPPIV; FAP; FAPA; Integral membrane serine protease; SEPR_HUMAN; Seprase;Prolyl endopeptidase FAP; Dipeptidyl peptidase FAP; Fibroblast activation protein alpha; FAPalpha; FAP alpha; Gelatine degradation protease FAP; Post-proline cleaving enzyme; Serine integral membrane protease; SIMP; Surface-expressed protease; Seprase;
抗原和靶标
免疫原:
A synthesized peptide derived from human FAP, corresponding to a region within the internal amino acids.
基因/基因ID:
文献引用
1). Spatial-Drug-Laden Protease-Activatable M1 Macrophage System Targets Lung Metastasis and Potentiates Antitumor Immunity. ACS nano, 2023
(PubMed: 36877635)
[IF=15.8]
2). Microvesicle-inspired oxygen-delivering nanosystem potentiates radiotherapy-mediated modulation of tumor stroma and antitumor immunity. Biomaterials, 2022
(PubMed: 36257260)
[IF=12.8]
3). Exosomal miR-500a-5p derived from cancer-associated fibroblasts promotes breast cancer cell proliferation and metastasis through targeting USP28. Theranostics, 2023
(PubMed: 33664871)
[IF=12.4]
4). FAP-catalyzed in situ self-assembly of magnetic resonance imaging probe for early and precise staging of liver fibrosis. Science advances, 2025
(PubMed: 40073128)
[IF=11.7]
5). Irinotecan/scFv co-loaded liposomes coaction on tumor cells and CAFs for enhanced colorectal cancer therapy. Journal of Nanobiotechnology, 2021
(PubMed: 34906155)
[IF=10.2]
6). Cancer-associated fibroblasts secrete CSF3 to promote TNBC progression via enhancing PGM2L1-dependent glycolysis reprogramming. Cell Death & Disease, 2025
[IF=9.6]
7). Tsp2 Facilitates Tumor-associated Fibroblasts Formation and Promotes Tumor Progression in Retroperitoneal Liposarcoma. International Journal of Biological Sciences, 2022
(PubMed: 35982904)
[IF=8.2]
8). IL-17a promotes hepatocellular carcinoma by increasing FAP expression in hepatic stellate cells via activation of the STAT3 signaling pathway. Cell death discovery, 2024
(PubMed: 38740736)
[IF=7.0]
Application: IF/ICC Species: Human Sample:
Fig. 2 IL-17a activates LX2 cell line, promotes their proliferation and migration, and inhibits their apoptosis. A Representative images indicating IL-17RA expression in the LX2 cell line. (Immunofluorescence staining, 200x, blue fluorescence, nuclei; red fluorescence, IL-17RA). B Representative images of quiescent (upper) and activated (lower) LX2 cells before and after IL-17a stimulation. C Cell proliferation curves of LX2 cells stimulated with 20 ng/ml IL-17a or treated with PBS, as determined by a CCK8 assay. D Proliferation rate of cells with IL-17a stimulation or PBS treatment, as determined by an EdU incorporation assay. E Representative images and statistical analysis of cell proliferation after IL-17a stimulation or PBS treatment by a plate colony formation assay. F Representative images of cell proliferation after IL-17a stimulation or PBS treatment, as shown by EdU incorporation (immunofluorescence staining, 200x, blue fluorescence, nuclei; red fluorescence, proliferating cells). G Representative images and statistical analysis of cell migration after IL-17a stimulation or PBS treatment in the Transwell assay. H Representative images of cell migration after IL-17a stimulation or PBS treatment in the wound healing assay. I Statistical analysis of wound closure after IL-17a stimulation or PBS treatment in the wound healing assay. J Apoptosis rate after IL-17a stimulation or PBS treatment, as determined by a TUNEL assay. K Representative images of apoptosis in the LX2 cell line after IL-17a stimulation or PBS treatment in the TUNEL assay (immunofluorescence staining, 200x, blue fluorescence, nuclei, green fluorescence, nuclei in cells undergoing apoptosis). L mRNA expression of several hepatic stellate cell-related genes in LX2 cell lines after IL-17a stimulation or PBS treatment. *, p
9). Radiation-induced FAP + fibroblasts are involved in keloid recurrence after radiotherapy. Frontiers in Cell and Developmental Biology, 2022
(PubMed: 36092734)
[IF=4.6]
10). Evaluation of a Novel Gd-FAPI Dimer Molecular Probe Targeting Fibroblast Activation Protein for Imaging of Solid Tumors. Molecular pharmaceutics, 2025
(PubMed: 39927594)
[IF=4.5]
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