DNA PK Antibody - #AF5340

>>买2送1 进行中>>
>>赠JD卡 进行中>>
(4)   (2)  
产品: DNA PK 抗体
货号: AF5340
描述: Rabbit polyclonal antibody to DNA PK
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse
预测: Pig, Bovine, Sheep
蛋白号: P78527
RRID: AB_2837825

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Polyclonal
特异性:
DNA PK Antibody detects endogenous levels of total DNA PK.
RRID:
AB_2837825
引用格式: Affinity Biosciences Cat# AF5340, RRID:AB_2837825.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DNA dependent protein kinase catalytic subunit; DNA PK catalytic subunit; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNAPK; DNAPK catalytic subunit; DNPK 1; DNPK1; Hyper radiosensitivity of murine scid mutation, complementing 1; Hyperradiosensitivity complementing 1, mouse, homolog of 1; HYRC 1; HYRC; HYRC1; IMD26; p350; p460; PKRDC; PRKDC; PRKDC_HUMAN; Protein Kinase DNA Activated Catalytic Polypeptide; XRCC 7; XRCC7;

抗原和靶标

免疫原:

A synthesized peptide derived from human DNA PK, corresponding to a region within C-terminal amino acids.

基因/基因ID:
PRKDC(5591)
描述:
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

文献引用

1). Di (2-ethyl-hexyl) phthalate disrupts placental growth in a dual blocking mode. Journal of hazardous materials, 2022 (PubMed: 34396966) [IF=12.2]
2). The dual HDAC-PI3K inhibitor CUDC-907 displays single-agent activity and synergizes with PARP inhibitor olaparib in small cell lung cancer. Journal of experimental & clinical cancer research : CR, 2020 (PubMed: 33069237) [IF=11.3]

Application: WB    Species: human    Sample: SCLC cells

Fig. 5 Effects of CUDC-907 and olaparib on the DNA repair pathways. a Representative images of Rad51 immunofluorescence staining in SCLC cells treated with drugs as indicated for 24 h. Scale bar, 10 μm. Quantification of Rad51 fluorescence intensities from three independent experiments was shown in right panels. b, c Western blot analysis of DDR proteins (b) and MYC paralogs and FoxM1 (c) in SCLC cells followed 10 nM CUDC-907 and 10 μM olaparib as single-agents or in combination treatment for 24 h. The antibody against MYCN was used to detect MYCN in H526 and H69 and anti-c-MYC was applied for the detection of c-MYC in rest of cell lines, β-actin was used as a loading control
3). Arsenic trioxide ameliorates experimental autoimmune encephalomyelitis in C57BL/6 mice by inducing CD4+ T cell apoptosis. Journal of Neuroinflammation, 2020 (PubMed: 32375831) [IF=9.3]
4). Suppression of fumarate hydratase activity increases the efficacy of cisplatin-mediated chemotherapy in gastric cancer. Cell Death & Disease, 2019 (PubMed: 31138787) [IF=8.1]

Application: WB    Species: human    Sample: SGC7901 and HGC27cells

Fig. 4| FH contributed to CDDP sensitivity via affecting DNA damage repair.g Immunoblotting for NHEJ pathway components in the indicated groups

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.