Fibronectin Antibody - #AF5335

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产品: Fibronectin 抗体
货号: AF5335
描述: Rabbit polyclonal antibody to Fibronectin
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit
蛋白号: P02751
RRID: AB_2837820

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Fibronectin Antibody detects endogenous levels of total Fibronectin.
RRID:
AB_2837820
引用格式: Affinity Biosciences Cat# AF5335, RRID:AB_2837820.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CIG; Cold insoluble globulin; Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED B; Fibronectin 1; FINC; FINC_HUMAN; FN; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor; MSF; Ugl-Y3;

抗原和靶标

免疫原:

A synthesized peptide derived from human Fibronectin, corresponding to a region within C-terminal amino acids.

基因/基因ID:
FN1(2335)
描述:
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

文献引用

1). Genomic and Transcriptomic Characterization of Natural Killer T Cell Lymphoma. CANCER CELL, 2020 (PubMed: 32183952) [IF=48.8]

Application: IF/ICC    Species: human    Sample: NK-92 cells

Figure 6. Biological Function of MGA and BRDT. (D) Immunofluorescence images of E-cadherin (green), fibronectin (red), or vimentin (red) in NK-92 cells transfected with MGA shRNAs or scramble. Cells were counterstained with DAPI (blue). (G) Immunofluorescence images of E-cadherin (green), fibronectin (red), or vimentin (red) in NK-92 cells transfected with pCMV6-BRDT (upper panel) or vector control (lower panel). Cells were counterstained with DAPI (blue).
2). A Constant Filgotinib Delivery Adhesive Platform Based on Polyethylene Glycol (PEG) Hydrogel for Accelerating Wound Healing via Restoring Macrophage Mitochondrial Homeostasis. Small (Weinheim an der Bergstrasse, Germany), 2024 (PubMed: 39679768) [IF=13.0]
3). Roles of gut microbiome-associated metabolites in pulmonary fibrosis by integrated analysis. NPJ biofilms and microbiomes, 2024 (PubMed: 39702426) [IF=9.2]
4). A novel trans-acting lncRNA of ACTG1 that induces the remodeling of ovarian follicles. International journal of biological macromolecules, 2023 (PubMed: 37276900) [IF=7.7]
5). Three-dimensional bioprinted cell-adaptive hydrogel with anisotropic micropores for enhancing skin wound healing. International journal of biological macromolecules, 2024 (PubMed: 39343255) [IF=7.7]
6). Renal tubular epithelial cell-derived Exosomal miR-330-3p plays a key role in fibroblast activation and renal fibrosis by regulating CREBBP. Stem cell research & therapy, 2025 (PubMed: 40269958) [IF=7.5]

Application: WB    Species: Mouse    Sample:

Fig. 3 Exosomes of tubular cell origin aggravate renal fibrosis. A Experimental design. Ctrl-Exo or UA-Exo from NRK-52E cells were injected (100 µL) into Adenine-induced model mice through the tail vein twice weekly for 8 weeks. B Mice were injected with DiR-labeled exosomes from NRK-52E cells via the tail vein. Fluorescent images of organs were acquired using an IVIS Spectrum Small Animal Optical Imaging System. Fluorescence luminescence of the heart, lungs, liver, spleen, and kidneys was assessed 12 h after injection. C, D Representative western blots (C) and quantitative data (D) of CD63, Hsp70, and TSG101 in the kidneys of model mice after injecting Ctrl-Exo or UA-Exo (n = 6). *p 
7). Huaier polysaccharides suppress triple-negative breast cancer metastasis and epithelial-mesenchymal transition by inducing autophagic degradation of Snail. Cell and Bioscience, 2021 (PubMed: 34481526) [IF=7.5]

Application: WB    Species: human    Sample: breast cancer cells

Fig. 1| PS-T inhibits invasion, migration and EMT in breast cancer cells in vitro and in vivo. d 4 T-1 and MDA-MB-231 cells are treated with 5 μg/mL PS-T for 24 h. The levels of each EMT marker are quantifed using the NIH ImageJ software. (mean ± SD, *P < 0.05 and **P < 0.01)

Application: WB    Species: Human    Sample: breast cancer cells

Fig. 1 PS-T inhibits invasion, migration and EMT in breast cancer cells in vitro and in vivo. a The invasiveness of 4 T-1 and MDA-MB-231 cells after treatment with PS-T at 5 μg/mL for 12 h is evaluated using Transwell invasion assays at 24 h (mean  ±  SD, ***P  <  0.001). b Migratory ability of 4 T-1 and MDA-MB-231 cells after treatment with PS-T at different concentrations is evaluated using scratch assays at different time points (mean  ±  SD, **P  <  0.01, ***P  <  0.001). c Mice are treated with normal saline (control), 25 μg/g or 100 μg/g PS-T by oral gavage every other day for 21 days (mean  ±  SD, *P  <  0.05, **P  <  0.01, scale bars: 100 μm). d 4 T-1 and MDA-MB-231 cells are treated with 5 μg/mL PS-T for 24 h. The levels of each EMT marker are quantified using the NIH ImageJ software. (mean  ±  SD, *P  <  0.05 and **P  <  0.01)
8). Mechanism of ketotifen fumarate inhibiting renal calcium oxalate stone formation in SD rats. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2022 (PubMed: 35643070) [IF=6.9]
9). Betulinic acid Attenuated Bleomycin-induced Pulmonary Fibrosis by Effectively Intervening Wnt/β-catenin Signaling. PHYTOMEDICINE, 2021 (PubMed: 33341025) [IF=6.7]
10). Bergenin attenuates bleomycin‐induced pulmonary fibrosis in mice via inhibiting TGF‐β1 signaling pathway. PHYTOTHERAPY RESEARCH, 2021 (PubMed: 34375009) [IF=6.1]
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