Desmin Antibody - #AF5334

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产品: Desmin 抗体
货号: AF5334
描述: Rabbit polyclonal antibody to Desmin
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 52 kDa; 54kD(Calculated).
蛋白号: P17661
RRID: AB_2837819

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WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
克隆:
Polyclonal
特异性:
Desmin Antibody detects endogenous levels of total Desmin.
RRID:
AB_2837819
引用格式: Affinity Biosciences Cat# AF5334, RRID:AB_2837819.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CMD1I; CSM1; CSM2; DES; DESM_HUMAN; Desmin; FLJ12025; FLJ39719; FLJ41013; FLJ41793; Intermediate filament protein; OTTHUMP00000064865;

抗原和靶标

免疫原:
Uniprot:

P17661(DESM_HUMAN) >>Visit HPA database.

基因/基因ID:
DES(1674)
描述:
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
序列:
MSQAYSSSQRVSSYRRTFGGAPGFPLGSPLSSPVFPRAGFGSKGSSSSVTSRVYQVSRTSGGAGGLGSLRASRLGTTRTPSSYGAGELLDFSLADAVNQEFLTTRTNEKVELQELNDRFANYIEKVRFLEQQNAALAAEVNRLKGREPTRVAELYEEELRELRRQVEVLTNQRARVDVERDNLLDDLQRLKAKLQEEIQLKEEAENNLAAFRADVDAATLARIDLERRIESLNEEIAFLKKVHEEEIRELQAQLQEQQVQVEMDMSKPDLTAALRDIRAQYETIAAKNISEAEEWYKSKVSDLTQAANKNNDALRQAKQEMMEYRHQIQSYTCEIDALKGTNDSLMRQMRELEDRFASEASGYQDNIARLEEEIRHLKDEMARHLREYQDLLNVKMALDVEIATYRKLLEGEESRINLPIQTYSALNFRETSPEQRGSEVHTKKTVMIKTIETRDGEVVSEATQQQHEVL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P17661 作为底物

Site PTM Type Enzyme
Y5 Phosphorylation
S6 Phosphorylation
S7 Phosphorylation
S8 Phosphorylation
S12 Phosphorylation Q96GD4 (AURKB) , P17612 (PRKACA) , Q13464 (ROCK1)
S13 Phosphorylation
T17 Phosphorylation P17612 (PRKACA) , Q13464 (ROCK1) , Q96GD4 (AURKB)
S28 Phosphorylation
S31 Phosphorylation
S32 Phosphorylation P06493 (CDK1)
S42 Phosphorylation
K43 Methylation
S45 Phosphorylation P17612 (PRKACA)
Y54 Phosphorylation
S57 Phosphorylation
T59 Phosphorylation
S60 Phosphorylation Q13464 (ROCK1) , Q96GD4 (AURKB) , P17612 (PRKACA)
S68 Phosphorylation
S72 Phosphorylation
T76 Phosphorylation Q13464 (ROCK1) , P17612 (PRKACA)
T77 Phosphorylation Q13464 (ROCK1)
T79 Phosphorylation
S81 Phosphorylation
S82 Phosphorylation
Y83 Phosphorylation
T106 Phosphorylation
K109 Acetylation
K109 Ubiquitination
Y122 Phosphorylation
Y155 Phosphorylation
T170 Phosphorylation
S231 Phosphorylation
K240 Acetylation
K241 Acetylation
Y281 Phosphorylation
Y296 Phosphorylation
S301 Phosphorylation
R315 Methylation
Y324 Phosphorylation
S330 Phosphorylation
Y331 Phosphorylation
T332 Phosphorylation
K339 Acetylation
S358 Phosphorylation
Y363 Phosphorylation
R386 Methylation
Y388 Phosphorylation
K407 Acetylation
K407 Sumoylation
K407 Ubiquitination
S414 Phosphorylation
Y423 Phosphorylation
S424 Phosphorylation
S432 Phosphorylation
T450 Phosphorylation

研究背景

功能:

Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity).

翻译修饰:

ADP-ribosylation prevents ability to form intermediate filaments.

Phosphorylation at Ser-7, Ser-28 and Ser-32 by CDK1, phosphorylation at Ser-60 by AURKB and phosphorylation at Thr-76 by ROCK1 contribute to efficient separation of desmin intermediate filaments during mitosis.

细胞定位:

Cytoplasm>Myofibril>Sarcomere>Z line. Cytoplasm. Cell membrane>Sarcolemma. Nucleus.
Note: Localizes in the intercalated disks which occur at the Z line of cardiomyocytes (PubMed:24200904, PubMed:26724190). Localizes in the nucleus exclusively in differentiating cardiac progenitor cells and premature cardiomyocytes (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homopolymer. Interacts with DST (By similarity). Interacts with MTM1. Interacts with EPPK1; interaction is dependent of higher-order structure of intermediate filament. Interacts with CRYAB. Interacts with NEB (via nebulin repeats 160-164). Interacts (via rod region) with NEBL (via nebulin repeats 1-5).

蛋白家族:

Belongs to the intermediate filament family.

研究领域

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

文献引用

1). Satellite cell-derived exosome-mediated delivery of microRNA-23a/27a/26a cluster ameliorates the renal tubulointerstitial fibrosis in mouse diabetic nephropathy. Acta Pharmacologica Sinica (PubMed: 37596398) [IF=8.2]
2). Human umbilical cord mesenchymal stem cells ameliorate erectile dysfunction in rats with diabetes mellitus through the attenuation of ferroptosis. Stem Cell Research & Therapy (PubMed: 36064453) [IF=7.5]
3). Exosomes derived from smooth muscle cells ameliorate diabetes‐induced erectile dysfunction by inhibiting fibrosis and modulating the NO/cGMP pathway. Journal of Cellular and Molecular Medicine (PubMed: 33009701) [IF=5.3]

Application: IF/ICC    Species: rat    Sample: CCSMCs

FIGURE 1 |Cell identification and exosome characterization. (C) Representative immunofluorescence results of CCSMCs show positive expression for α-SMA and desmin. Scale bars = 50 μm
4). Bi-phasic effect of gelatin in myogenesis and skeletal muscle regeneration. Disease Models & Mechanisms (PubMed: 34821368) [IF=4.3]
5). Integrated Network Pharmacology and Cellular Assay to Explore the Mechanisms of Selenized Tripterine Phytosomes (Se@Tri-PTs) Alleviating Podocyte Injury in Diabetic Nephropathy. Current pharmaceutical design (PubMed: 37961864) [IF=3.1]

Application: WB    Species: Mouse    Sample: podocytes

Fig. (7). Expressions of nephrin and desmin in podocytes treated for 48 h and quantified by western blot. **P < 0.01, compared to the NG group. Abbreviations: NG, normal glucose; DM, D-mannitol (30 mmol/L); HG, high glucose (30 mmol/L). (A higher resolution/colour version of this figure is available in the electronic copy of the article).

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