PIAS4 Antibody - #AF5329

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产品: PIAS4 抗体
货号: AF5329
描述: Rabbit polyclonal antibody to PIAS4
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Dog, Chicken
蛋白号: Q8N2W9
RRID: AB_2837814

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
PIAS4 Antibody detects endogenous levels of total PIAS4.
RRID:
AB_2837814
引用格式: Affinity Biosciences Cat# AF5329, RRID:AB_2837814.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

E3 SUMO protein ligase PIAS4; E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; PAIASgamma; PIAS 4; PIAS gamma; PIAS-gamma; Pias4; PIAS4_HUMAN; PIASG; PIASgamma; PIASy; Protein inhibitor of activated STAT 4; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; Protein inhibitor of activated STAT protein PIASy; Zinc finger MIZ type containing 6; ZMIZ 6; ZMIZ6;

抗原和靶标

免疫原:

A synthesized peptide derived from human PIAS4, corresponding to a region within C-terminal amino acids.

基因/基因ID:
PIAS4(51588)
描述:
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway.

研究领域

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

文献引用

1). Role of SUMOylation of STAT1 in tubular epithelial‑mesenchymal transition induced by high glucose. Molecular Medicine Reports, 2023 (PubMed: 36601740) [IF=3.4]

Application: WB    Species: Human    Sample: HK-2 cells

Figure 4. High glucose upregulates STAT1 SUMOylation in HK-2 cells. (A) Co-IP suggested that the binding of STAT1 and UBC9 was upregulated under high-glucose conditions. The data were analyzed by Student's t-test. (B) Efficiency of UBC9 knockdown was analyzed by western blotting and was semi-quantified using densitometry. The data were analyzed by one-way ANOVA and Tukey post hoc test. (C and D) Co-IP suggested that the SUMOylation of STAT1 was enhanced under high-glucose conditions. The data were analyzed by one-way ANOVA and Tukey post hoc test. (E) Binding of STAT1 and PIASs was analyzed. Results suggested that PIAS4 mediated the upregulation of STAT1 SUMOylation. The data were analyzed by Student's t-test. (F) Efficiency of PIAS4 knockdown was analyzed by western blotting and was semi-quantified using densitometry. The data were analyzed by one-way ANOVA and LSD post hoc test. The effects of PIAS4 siRNA transfection on STAT1 SUMOylation (G) were analyzed by co-IP and (H) were semi-quantified using densitometry. The results suggested that PIAS4 knockdown inhibited STAT1 SUMOylation. The data were analyzed by one-way ANOVA and Tukey post hoc test. IP:STAT1 means the cell lysate was incubated with STAT1 antibody. Input was used as a positive control to detect the presence of target proteins. n=3. *P

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