MLCK Antibody - #AF5314

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产品: MLCK 抗体
货号: AF5314
描述: Rabbit polyclonal antibody to MLCK
应用: WB
文献验证: WB
反应: Human, Mouse, Rat
预测: Bovine, Sheep, Rabbit, Dog, Chicken
蛋白号: Q15746
RRID: AB_2837799

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
MLCK Antibody detects endogenous levels of total MLCK.
RRID:
AB_2837799
引用格式: Affinity Biosciences Cat# AF5314, RRID:AB_2837799.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

deglutamylated form; DKFZp686I10125; EC 2.7.11.18; FLJ12216; Kinase related protein; Kinase-related protein; KRP; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; MYLK; MYLK_HUMAN; MYLK1; Myosin light chain kinase; Myosin light polypeptide kinase; OTTHUMP00000180642; OTTHUMP00000180643; smMLCK; smooth muscle; Smooth muscle myosin light chain kinase; Telokin;

抗原和靶标

免疫原:

A synthesized peptide derived from human MLCK, corresponding to a region within C-terminal amino acids.

基因/基因ID:
MYLK(4638)
描述:
Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Digestive system > Gastric acid secretion.

文献引用

1). Banana Starch Nanoparticles Disrupt the Integrity of the Intestinal Barrier by Opening Tight Junctions in Mice. Small (Weinheim an der Bergstrasse, Germany), 2024 (PubMed: 39668449) [IF=13.0]
2). Sanguinarine Improves Intestinal Health in Grass Carp Fed High-Fat Diets: Involvement of Antioxidant, Physical and Immune Barrier, and Intestinal Microbiota. Antioxidants, 2023 (PubMed: 37507906) [IF=7.0]

Application: WB    Species: grass carp    Sample:

Figure 5 Effects of adding sanguinarine to a high-fat diet on MLCK gene expression and protein expression of grass carp. (A) relative gene expression level of mlck; (B) relative protein level of MLCK; (C) Blot of MLCK and β-actin. Bars represent the mean ± SE. Significant differences are expressed by different superscripts (p < 0.05).
3). Multi-omic analysis revealed the therapeutic mechanisms of Alpinia oxyphylla fructus water extract against bladder overactivity in spontaneously hypertensive rats. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 37976696) [IF=6.7]
4). A comparative study of the ameliorative effects of hyaluronic acid oligosaccharides and hyaluronic acid on DSS-induced colitis in mice and research on relevant mechanisms. Food & function, 2023 (PubMed: 37366083) [IF=5.1]
5). Multi-omics approaches revealed the therapeutic mechanisms of Suo-Quan-Wan for treating overactive bladder in spontaneously hypertensive rats. Journal of ethnopharmacology, 2024 (PubMed: 37604331) [IF=4.8]
6). Qingchang Wenzhong Decoction ameliorates intestinal inflammation and intestinal barrier dysfunction in ulcerative colitis via the GC-C signaling pathway. Journal of ethnopharmacology, 2024 (PubMed: 38043755) [IF=4.8]
7). Fasudil Dichloroacetate Alleviates SU5416/Hypoxia-Induced Pulmonary Arterial Hypertension by Ameliorating Dysfunction of Pulmonary Arterial Smooth Muscle Cells. Drug Design Development and Therapy, 2021 (PubMed: 33935492) [IF=4.7]

Application: WB    Species: Human    Sample: pulmonary arterial smooth muscle cell

Figure 6 FDCA inhibited hypoxia-induced activation of the intracellular Ca2+ signaling pathway. (A) Representative images of [Ca2+]i. (B) Quantitative analysis of Fluo-4 AM fluorescence intensity. (C) Representative Western blot bands. (D) Quantitative analysis of CaMK II/p-CaMK II. (E) Quantitative analysis of p-MLCK/MLCK. Data are expressed as mean ± SD (n=3-5). *P<0.05, **P<0.01 vs normoxia control group; ##P<0.01 vs hypoxia group; &P<0.05, &&P<0.01 vs hypoxia+FDCA group, ns (not significant).
8). The Probiotic Lactobacillus paracasei Ameliorates Diarrhea Cause by Escherichia coli O8 via Gut Microbiota Modulation1. Frontiers in Nutrition, 2022 (PubMed: 35662940) [IF=4.0]

Application: WB    Species: Mouse    Sample:

FIGURE 8 Protein expression levels of IκBα, p65, MLC2, MLCK and β-actin on days 8 (A) and 15 (B). *Representative compared with CK group. *P < 0.05, **P < 0.01. ▲Representative compared with NC group. ▲P < 0.05, ▲▲P < 0.01 as determined by Student’s t-test.
9). Long-term tuina can inhibit the occurrence of gastroparesis by protecting gastrointestinal function in diabetic rats. Frontiers in endocrinology, 2025 (PubMed: 40636716) [IF=3.9]

Application: WB    Species: Rat    Sample:

Figure 5. mRNA and protein expression of CaM and MLCK in gastric antrum tissues of rats in each group. (A, B) Gastric antrum tissue CaM and MLCK mRNA expression. (C–E) Expression of CaM and MLCK protein in gastric antrum tissue. Data are expressed as mean ± standard deviation, n = 5. NC, Normal control group; DM, Diabetes mellitus group; DM + GT, Diabetes mellitus + gentle touch group; DM + TT, Diabetes mellitus + tuina treatment group. CaM, Calmodulin; MLCK, Myosin light chain kinase.
10). Lifibrate attenuates blood-brain barrier damage following ischemic stroke via the MLCK/p-MLC/ZO-1 axis. Aging, 2024 (PubMed: 38546384) [IF=3.9]
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