MLCK Antibody - #AF5314

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产品: MLCK 抗体
货号: AF5314
描述: Rabbit polyclonal antibody to MLCK
应用: WB
文献验证: WB
反应: Human, Mouse, Rat
预测: Bovine, Sheep, Rabbit, Dog, Chicken
蛋白号: Q15746
RRID: AB_2837799

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
MLCK Antibody detects endogenous levels of total MLCK.
RRID:
AB_2837799
引用格式: Affinity Biosciences Cat# AF5314, RRID:AB_2837799.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

deglutamylated form; DKFZp686I10125; EC 2.7.11.18; FLJ12216; Kinase related protein; Kinase-related protein; KRP; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; MYLK; MYLK_HUMAN; MYLK1; Myosin light chain kinase; Myosin light polypeptide kinase; OTTHUMP00000180642; OTTHUMP00000180643; smMLCK; smooth muscle; Smooth muscle myosin light chain kinase; Telokin;

抗原和靶标

免疫原:

A synthesized peptide derived from human MLCK, corresponding to a region within C-terminal amino acids.

基因/基因ID:
MYLK(4638)
描述:
Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Digestive system > Gastric acid secretion.

文献引用

1). Banana Starch Nanoparticles Disrupt the Integrity of the Intestinal Barrier by Opening Tight Junctions in Mice. Small (Weinheim an der Bergstrasse, Germany), 2024 (PubMed: 39668449) [IF=13.0]
2). Sanguinarine Improves Intestinal Health in Grass Carp Fed High-Fat Diets: Involvement of Antioxidant, Physical and Immune Barrier, and Intestinal Microbiota. Antioxidants, 2023 (PubMed: 37507906) [IF=7.0]

Application: WB    Species: grass carp    Sample:

Figure 5 Effects of adding sanguinarine to a high-fat diet on MLCK gene expression and protein expression of grass carp. (A) relative gene expression level of mlck; (B) relative protein level of MLCK; (C) Blot of MLCK and β-actin. Bars represent the mean ± SE. Significant differences are expressed by different superscripts (p < 0.05).
3). Multi-omic analysis revealed the therapeutic mechanisms of Alpinia oxyphylla fructus water extract against bladder overactivity in spontaneously hypertensive rats. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 37976696) [IF=6.7]
4). A comparative study of the ameliorative effects of hyaluronic acid oligosaccharides and hyaluronic acid on DSS-induced colitis in mice and research on relevant mechanisms. Food & function, 2023 (PubMed: 37366083) [IF=5.1]
5). Multi-omics approaches revealed the therapeutic mechanisms of Suo-Quan-Wan for treating overactive bladder in spontaneously hypertensive rats. Journal of ethnopharmacology, 2024 (PubMed: 37604331) [IF=4.8]
6). Qingchang Wenzhong Decoction ameliorates intestinal inflammation and intestinal barrier dysfunction in ulcerative colitis via the GC-C signaling pathway. Journal of ethnopharmacology, 2024 (PubMed: 38043755) [IF=4.8]
7). Fasudil Dichloroacetate Alleviates SU5416/Hypoxia-Induced Pulmonary Arterial Hypertension by Ameliorating Dysfunction of Pulmonary Arterial Smooth Muscle Cells. Drug Design Development and Therapy, 2021 (PubMed: 33935492) [IF=4.7]

Application: WB    Species: Human    Sample: pulmonary arterial smooth muscle cell

Figure 6 FDCA inhibited hypoxia-induced activation of the intracellular Ca2+ signaling pathway. (A) Representative images of [Ca2+]i. (B) Quantitative analysis of Fluo-4 AM fluorescence intensity. (C) Representative Western blot bands. (D) Quantitative analysis of CaMK II/p-CaMK II. (E) Quantitative analysis of p-MLCK/MLCK. Data are expressed as mean ± SD (n=3-5). *P<0.05, **P<0.01 vs normoxia control group; ##P<0.01 vs hypoxia group; &P<0.05, &&P<0.01 vs hypoxia+FDCA group, ns (not significant).
8). The Probiotic Lactobacillus paracasei Ameliorates Diarrhea Cause by Escherichia coli O8 via Gut Microbiota Modulation1. Frontiers in Nutrition, 2022 (PubMed: 35662940) [IF=4.0]

Application: WB    Species: Mouse    Sample:

FIGURE 8 Protein expression levels of IκBα, p65, MLC2, MLCK and β-actin on days 8 (A) and 15 (B). *Representative compared with CK group. *P < 0.05, **P < 0.01. ▲Representative compared with NC group. ▲P < 0.05, ▲▲P < 0.01 as determined by Student’s t-test.
9). Lifibrate attenuates blood-brain barrier damage following ischemic stroke via the MLCK/p-MLC/ZO-1 axis. Aging, 2024 (PubMed: 38546384) [IF=3.9]
10). Dietary sanguinarine ameliorates growth impairment and intestinal dysfunction in Ctenopharyngodon idellus fed a high cottonseed meal diet. Aquaculture, 2023 [IF=3.9]
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