Cytochrome P450 1A1/2 Antibody - #AF5312

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产品: Cytochrome P450 1A1/2 抗体
货号: AF5312
描述: Rabbit polyclonal antibody to Cytochrome P450 1A1/2
应用: WB IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
蛋白ID: P04798 | P05177
RRID: AB_2837797

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Cytochrome P450 1A1/2 Peptide
相关下载
抗原肽/阻断肽MS报告
抗原肽/阻断肽HPLC报告
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
Cytochrome P450 1A1/2 Antibody detects endogenous levels of total Cytochrome P450 1A1/2.
RRID:
AB_2837797
引用格式: Affinity Biosciences Cat# AF5312, RRID:AB_2837797.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AHH; AHRR; Aryl hydrocarbon hydroxylase; CP11; CP1A1_HUMAN; CYP 1; CYP1; cyp1a1; CYPIA1; Cytochrome P1 450 dioxin inducible; Cytochrome P1-450; Cytochrome P450 1A1; Cytochrome P450 family 1 subfamily A polypeptide 1; Cytochrome P450 form 6; Cytochrome P450 subfamily I (aromatic compound inducible) polypeptide 1; Cytochrome P450-C; Cytochrome P450-P1; Flavoprotein-linked monooxygenase; Microsomal monooxygenase; P1 450; P450 C; P450 form 6; P450 P1; P450DX; Xenobiotic monooxygenase; Aryl hydrocarbon hydroxylase; CP 12; CP12; CP1A2_HUMAN; CYP1A2; CYPIA2; Cytochrome P(3)450; Cytochrome P450 1A2; Cytochrome P450 4; Cytochrome P450 family 1 polypeptide 2; Cytochrome P450 family 1 subfamily A polypeptide 2; Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2; Cytochrome P450-P3; Cytochrome P4501A2; Dioxin inducable P3 450; Flavoprotein linked monooxygenase; Microsomal monooxygenase; P(3)450; P3 450; P450 4; P450 form 4; P450 P3; P450(PA); Xenobiotic monooxygenase;

抗原和靶标

免疫原:

A synthesized peptide derived from human Cytochrome P450 1A1/2, corresponding to a region within N-terminal amino acids.

基因/基因ID:
CYP1A1(1543) | CYP1A2(1544)
描述:
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

研究领域

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Biosynthesis of other secondary metabolites > Caffeine metabolism.

· Metabolism > Amino acid metabolism > Tryptophan metabolism.

· Metabolism > Lipid metabolism > Linoleic acid metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

文献引用

1). LncRNA MACC1-AS1 induces gemcitabine resistance in pancreatic cancer cells through suppressing ferroptosis. Cell death discovery, 2024 (PubMed: 38413579) [IF=7.0]
2). CIRBP Enhances the Function of Yak Cumulus Cells by Activating AMPK/mTOR-Mediated Mitophagy. Biomolecules, 2025 (PubMed: 40563401) [IF=5.5]

Application: WB    Species: Yak    Sample: Yak Cumulus Cells

Figure 3. Overexpression of CIRBP improves YCC function. (A) Steroid-synthesis-related factors expression level after CIRBP overexpression, n = 4. (B) The mRNA expression level of cumulus-diffusion-related factors, n = 4. (C) The secretion of E2 and P4, n = 4. (D) E2 and P4 synthesis-related protein bands and analysis, n = 3. (E) Cumulus-diffusion-related protein bands and analysis, n = 3. (F) Immunofluorescence detection of cumulus-diffusion-related proteins. Values are mean ± SE, *: p < 0.05, **: p < 0.01, NS: no difference. Original Western blot images are provided in the Supplementary Materials.
3). GDU-952, a novel AhR agonist ameliorates skin barrier abnormalities and immune dysfunction in DNFB-induced atopic dermatitis in mice. Biochemical pharmacology, 2023 (PubMed: 37778446) [IF=5.3]
4). Advanced oxidation protein products downregulate CYP1A2 and CYP3A4 expression and activity via the NF-κB-mediated signaling pathway in vitro and in vivo. Laboratory Investigation, 2021 (PubMed: 34031539) [IF=5.1]
5). Gut microbial metabolite indole-3-propionic acid alleviates polycystic ovary syndrome in mice by regulating the AhR-NLRP3 axis. International immunopharmacology, 2025 (PubMed: 39826449) [IF=4.8]
6). Honokiol attenuates diabetes by enriching Akkermansia muciniphila andregulating tryptophan metabolism in mice. Chinese journal of natural medicines, 2026 (PubMed: 41571367) [IF=4.0]
7). Subacute ruminal acidosis downregulates FOXA2, changes oxidative status, and induces autophagy in the livers of dairy cows fed a high-concentrate diet. Journal of Dairy Science, 2023 (PubMed: 36631320) [IF=3.7]
8). Thiophene-based styrene derivative improves colitis symptoms in DSS-induced BALB/C mice through AhR-mediated gut barrier function and inflammatory responses. Toxicology and applied pharmacology, 2026 (PubMed: 41638505) [IF=3.3]
9). CIRBP Increases the synthesis and secretion of steroid hormones by in yak granulaso cells. The Journal of steroid biochemistry and molecular biology, 2024 (PubMed: 38143009) [IF=2.7]
10). Exosomes Derived from Yak Follicular Fluid Increase 2-Hydroxyestradiol Secretion by Activating Autophagy in Cumulus Cells. Animals, 2022 (PubMed: 36428401) [IF=2.7]

Application: WB    Species: Yak    Sample: YCCs

Figure 5.Yak follicular fluid exosomes could increase 2-OHE2 secretion in YCCs. (A) Immunofluorescence staining of CYP19A1 and CYP1A1 proteins in YCCs. Scale bar represents 50 μm. (B) Expression of 2-OHE2 secretion-related genes CYP17A1 (B1), CYP19A1 (B2), CYP1A1 (B3) and CYP1B1 (B4) in YCCs using the qPCR assay. (C) Western blot analysis of the protein expression in YCCs (C: Control, E: Yak follicular fluid exosomes) (See Figure S2). (D) ELISA kit analyses of the concentrations of estradiol (pg/mL) in the cell supernatant. (E) Quantitative Western blot results for CYP17A1 (E1), CYP19A1 (E2), CYP1A1 (E3) and CYP1B1 (E4). Data are expressed as the mean ± SD. n = 6. NS means no significant difference. * p < 0.05 and ** p < 0.01 indicate significant differences between the yak follicular fluid exosome treatments and the control.

Application: IF/ICC    Species: Yak    Sample: YCCs

Figure 5.Yak follicular fluid exosomes could increase 2-OHE2 secretion in YCCs. (A) Immunofluorescence staining of CYP19A1 and CYP1A1 proteins in YCCs. Scale bar represents 50 μm. (B) Expression of 2-OHE2 secretion-related genes CYP17A1 (B1), CYP19A1 (B2), CYP1A1 (B3) and CYP1B1 (B4) in YCCs using the qPCR assay. (C) Western blot analysis of the protein expression in YCCs (C: Control, E: Yak follicular fluid exosomes) (See Figure S2). (D) ELISA kit analyses of the concentrations of estradiol (pg/mL) in the cell supernatant. (E) Quantitative Western blot results for CYP17A1 (E1), CYP19A1 (E2), CYP1A1 (E3) and CYP1B1 (E4). Data are expressed as the mean ± SD. n = 6. NS means no significant difference. * p < 0.05 and ** p < 0.01 indicate significant differences between the yak follicular fluid exosome treatments and the control.
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