NDUFA4 Antibody - #AF5277

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产品: NDUFA4 抗体
货号: AF5277
描述: Rabbit polyclonal antibody to NDUFA4
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Chicken
蛋白号: O00483
RRID: AB_2837763

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
NDUFA4 Antibody detects endogenous levels of total NDUFA4.
RRID:
AB_2837763
引用格式: Affinity Biosciences Cat# AF5277, RRID:AB_2837763.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CI 9k; CI-MLRQ; Complex I 9kDa subunit; Complex I-MLRQ; MLRQ; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 9kD; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4; NADH-ubiquinone oxidoreductase MLRQ subunit; NDUA4_HUMAN; NDUFA4;

抗原和靶标

免疫原:

A synthesized peptide derived from human NDUFA4, corresponding to a region within C-terminal amino acids.

基因/基因ID:
NDUFA4(4697)
描述:
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

研究领域

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

文献引用

1). Oligodendrocyte-astrocyte crosstalk in Parkinson's disease mediates neuronal ferroptosis via the FGF signaling pathway. NPJ Parkinson's disease, 2025 (PubMed: 40410211) [IF=8.7]

Application: WB    Species: Mouse    Sample:

Fig. 7: Oxidative phosphorylation and ferroptosis processes are activated in oligodendrocytes and astrocytes. A The results of the Western Blot show that the protein expression levels of Slc25a4, Ndufa4, and Atp6v0c, which are related to mitochondrial oxidative phosphorylation, are significantly increased in astrocytes and oligodendrocytes under PD conditions. Additionally, in PD astrocytes, there is a significant decrease in the expression of the Mt1 protein. B The results of the ELISA kit show that the Ca2+ content in astrocytes of PD mice is significantly increased. At the same time, the iron ion content in the SN of mice is significantly elevated, while the GSH content, total SOD activity, and MDA content are significantly decreased, indicating the presence of high levels of ROS and the activation of ferroptosis processes in the SN of PD mice.

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