| 产品: | BUB3 抗体 |
| 货号: | AF5252 |
| 描述: | Rabbit polyclonal antibody to BUB3 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 37 kDa; 37kD(Calculated). |
| 蛋白号: | O43684 |
| RRID: | AB_2837738 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5252, RRID:AB_2837738.
展开/折叠
Aa2-050; AU019800; AU021329; AU043350; AW146323; BUB 3; BUB 3L; BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog; bub3; BUB3 budding uninhibited by benzimidazoles 3 homolog; BUB3 mitotic checkpoint protein; BUB3_HUMAN; BUB3L; Budding uninhibited by benomyl; Budding uninhibited by benzimidazoles 3; Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae); Budding uninhibited by benzimidazoles 3 homolog (yeast); Budding uninhibited by benzimidazoles 3 homolog; Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of; C78067; hBUB3; Mitotic checkpoint component; Mitotic checkpoint protein bub3; OTTHUMP00000020679; OTTHUMP00000020680; WD repeat type I transmembrane protein A72.5;
抗原和靶标
- O43684 BUB3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPEVNVMVTGSWDQTVKLWDPRTPCNAGTFSQPEKVYTLSVSGDRLIVGTAGRRVLVWDLRNMGYVQQRRESSLKYQTRCIRAFPNKQGYVLSSIEGRVAVEYLDPSPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAFSNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDAETKPKSPCT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O43684 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Sumoylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| S18 | Phosphorylation | PR:O43683 (hBUB1) | Uniprot |
| S19 | Phosphorylation | O43683 (BUB1) | Uniprot |
| K21 | Ubiquitination | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| T86 | Phosphorylation | Uniprot | |
| R126 | Methylation | Uniprot | |
| T127 | Phosphorylation | Uniprot | |
| C129 | S-Nitrosylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| Y141 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| R149 | Methylation | Uniprot | |
| T154 | Phosphorylation | Uniprot | |
| K179 | Acetylation | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| R186 | Methylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| Y194 | Phosphorylation | Uniprot | |
| Y207 | Phosphorylation | P14618 (PKM) | Uniprot |
| S211 | Phosphorylation | Uniprot | |
| K216 | Acetylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K227 | Sumoylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K322 | Acetylation | Uniprot | |
| K322 | Sumoylation | Uniprot | |
| K322 | Ubiquitination | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| C327 | S-Nitrosylation | Uniprot | |
| T328 | Phosphorylation | Uniprot |
研究背景
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Poly-ADP-ribosylated by PARP1.
Nucleus. Chromosome>Centromere>Kinetochore.
Note: Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Interacts with ZNF207/BuGZ; leading to promote stability and kinetochore loading of BUB3.
Belongs to the WD repeat BUB3 family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.