Smad4 Antibody - #AF5247

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产品: Smad4 抗体
货号: AF5247
描述: Rabbit polyclonal antibody to Smad4
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: Q13485
RRID: AB_2837733

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Smad4 Antibody detects endogenous levels of total Smad4.
RRID:
AB_2837733
引用格式: Affinity Biosciences Cat# AF5247, RRID:AB_2837733.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

(Small) Mothers Against Decapentaplegic; Deleted in Pancreatic Carcinoma 4; Deleted in Pancreatic Carcinoma; Deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; DPC 4; DPC4; hSMAD4; JIP; MAD homolog 4; MAD mothers against decapentaplegic Drosophila homolog 4; MAD mothers against decapentaplegic homolog 4; MADH 4; MADH4; Med; Medea; Mothers against decapentaplegic homolog 4; Mothers against decapentaplegic, Drosophila, homolog of, 4; Mothers against DPP homolog 4; MYHRS; OTTHUMP00000163548; SMA- and MAD-related protein 4; SMAD 4; SMAD family member 4; SMAD mothers against DPP homolog 4; SMAD4; SMAD4_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human Smad4, corresponding to a region within N-terminal amino acids.

基因/基因ID:
SMAD4(4089)
描述:
Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

文献引用

1). MCU regulating bone remodeling and osteogenic function through mitochondrial calcium homeostasis and oxidative stress alteration. Free radical biology & medicine, 2025 (PubMed: 40389181) [IF=7.1]
2). Dietary protein levels changed the hardness of muscle by acting on muscle fiber growth and the metabolism of collagen in sub-adult grass carp (Ctenopharyngodon idella). Journal of Animal Science and Biotechnology, 2022 (PubMed: 36002862) [IF=7.0]

Application: WB    Species: grass carp    Sample: muscle

Fig. 3 Western blot analysis of relative protein expression in the muscle of grass carp. A Collagen transcription related factors; B Related factors regulating collagen mRNA translation; C Collagen degradation related factors. Values are means ± SD and n = 6 for each group. Different letters are significantly different (P < 0.05)
3). LINC00909 up-regulates pluripotency factors and promotes cancer stemness and metastasis in pancreatic ductal adenocarcinoma by targeting SMAD4. Biology direct, 2024 (PubMed: 38504385) [IF=5.5]

Application: WB    Species: Human    Sample: PDAC cells

Fig. 5. LINC00909 enhances the stemness of pancreatic ductal adenocarcinoma (PDAC) by inhibiting SMAD4 expression. (A–C) RT-qPCR analysis was performed to detect the mRNA levels of SMAD4 in LINC00909-overexpressing cells (A) and LINC00909-knockdown cells (B, C). (D–F) Western blotting analysis was conducted to examine the protein expression of SMAD4 in PDAC cells. (G, H) The efficiency of SMAD4 knockdown in PANC-1 (G) and AsPC-1 cells (H) was detected by RT-qPCR. (I, J) Representative images of sphere-formation assays in PANC-1/KD and AsPC-1/KD cells transfected with siSMAD4-2. (K, L) The protein levels of SMAD4, KLF4, c-Myc, JNK, p-JNK, JUN and p-JUN in PANC-1/LINC00909-KD (K) and AsPC-1/LINC00909-KD cells (L) transfected with siSMAD4-2 were analyzed by western blotting. (M) PANC-1 cells transfected with EV or vector encoding LINC00909 were treated with actinomycin D (1 mg/ml) at the indicated time point. (N, O) PANC-1 or AsPC-1 cells transfected with siNC, siLINC00909-3, or siLINC00909-6 were treated with actinomycin D (1 mg/ml) at the indicated time point. Total RNA was extracted and analyzed by RT-qPCR to examine the relative levels of SMAD4 mRNA (M–O). All *P 
4). Novel polysaccharide identified from Cortinarius purpurascens demonstrated anti-fibrosis effects in cardiac fibroblasts. Food Bioscience, 2023 [IF=4.8]
5). Jiawei Maxing Shigan Tang alleviates radiation-induced lung injury via TGF-β1/Smad signaling pathway mediated by regulatory T cells. Journal of ethnopharmacology, 2024 (PubMed: 37944875) [IF=4.8]

Application: WB    Species: Human    Sample:

Fig. 1. Evaluation of the effects of CD4+CD25+ Tregs on TGF-β1/Smad pathway and the EMT process. (A) The release of TGF-β1 from Tregs was detected by ELISA. (B–F) Protein levels of Smad4, Vimentin, α-SMA, and E-cadherin in type II AECs co-cultured with Tregs supernatant were detected by Western blot. *P＜0.05, **P＜0.01, ***P＜0.001, n = 3.
6). Enhancement of flavor and healthcare substances, mouthfeel parameters and collagen synthesis in the muscle of on-growing grass carp (Ctenopharyngodon idella) fed with graded levels of glutamine. Aquaculture, 2020 [IF=3.9]
7). Enhancements in flavor substances, mouthfeel characteristics and collagen synthesis in the muscle of sub-adult grass carp (Ctenopharyngodon Idella): Application of a dietary lysine nutrition strategy. Aquaculture, 2023 [IF=3.9]
8). Astragaloside IV inhibits cell proliferation in vulvar squamous cell carcinoma through the TGF‐β/Smad signaling pathway. Dermatologic Therapy, 2019 (PubMed: 30536730) [IF=3.7]

Application: WB    Species: human    Sample: SW962 cells

Figure 3. |AS-Ⅳ reverses TGF-β/Smad signaling abnormalities and inhibits TGF-β1-mediated cell proliferation and anti-apoptosis in SW962 cells. SW962 cells were treated with TGF-β1 (10 ng/ml) alone or in combination with AS-Ⅳ (800 μg/ml) for 24 h and 48 h, respectively.Western blot assays evaluated the protein levels of TGF-βRI, TGF-βRII,Smad2/3, p-Smad2/3 and Smad4 (A,B).
9). Comprehensive upstream and downstream regulatory analyses identify miR-675-3p as a potential prognostic biomarker in melanoma. Human Cell, 2021 (PubMed: 33400243) [IF=3.4]

Application: WB    Species: Human    Sample: melanoma A375 cells

Fig. 7 miR-675-3p may promote cell proliferation, regulate the cell cycle, and activate the TGF-β, HIF-1 signaling pathways. a miR- 675-3p facilitated the G0/G1–G2/M transition in human melanoma A375 cells, b miR-675-3p promoted cell proliferation. c The TGFβ2, Smad2/3, Smad4, and HIF1A protein levels in the TGF-β/SMAD and HIF-1 signaling pathways were signifcantly higher, while TGF β1 was decreased relative to the negative control group
10). Huangqi decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathway. BMC complementary medicine and therapies, 2023 (PubMed: 37365530) [IF=3.3]

Application: WB    Species: Mouse    Sample: kidneys

Fig. 6 HQD downregulated the expression of the Smad1 protein in the kidneys of db/db mice. A. The protein expression levels of P-Smad1/5/9, Smad1 and Smad4 in the kidney cortex by Western blot assay. B. Statistical results of the expression level of Smad1, Values are mean ± SEM (n = 3), *P 
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