Smad4 Antibody - #AF5247

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产品: Smad4 抗体
货号: AF5247
描述: Rabbit polyclonal antibody to Smad4
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: Q13485
RRID: AB_2837733

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Smad4 Antibody detects endogenous levels of total Smad4.
RRID:
AB_2837733
引用格式: Affinity Biosciences Cat# AF5247, RRID:AB_2837733.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

(Small) Mothers Against Decapentaplegic; Deleted in Pancreatic Carcinoma 4; Deleted in Pancreatic Carcinoma; Deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; DPC 4; DPC4; hSMAD4; JIP; MAD homolog 4; MAD mothers against decapentaplegic Drosophila homolog 4; MAD mothers against decapentaplegic homolog 4; MADH 4; MADH4; Med; Medea; Mothers against decapentaplegic homolog 4; Mothers against decapentaplegic, Drosophila, homolog of, 4; Mothers against DPP homolog 4; MYHRS; OTTHUMP00000163548; SMA- and MAD-related protein 4; SMAD 4; SMAD family member 4; SMAD mothers against DPP homolog 4; SMAD4; SMAD4_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human Smad4, corresponding to a region within N-terminal amino acids.

基因/基因ID:
SMAD4(4089)
描述:
Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

文献引用

1). Dietary protein levels changed the hardness of muscle by acting on muscle fiber growth and the metabolism of collagen in sub-adult grass carp (Ctenopharyngodon idella). Journal of Animal Science and Biotechnology, 2022 (PubMed: 36002862) [IF=7.0]

Application: WB    Species: grass carp    Sample: muscle

Fig. 3 Western blot analysis of relative protein expression in the muscle of grass carp. A Collagen transcription related factors; B Related factors regulating collagen mRNA translation; C Collagen degradation related factors. Values are means ± SD and n = 6 for each group. Different letters are significantly different (P < 0.05)
2). LINC00909 up-regulates pluripotency factors and promotes cancer stemness and metastasis in pancreatic ductal adenocarcinoma by targeting SMAD4. Biology direct, 2024 (PubMed: 38504385) [IF=5.5]

Application: WB    Species: Human    Sample: PDAC cells

Fig. 5. LINC00909 enhances the stemness of pancreatic ductal adenocarcinoma (PDAC) by inhibiting SMAD4 expression. (A–C) RT-qPCR analysis was performed to detect the mRNA levels of SMAD4 in LINC00909-overexpressing cells (A) and LINC00909-knockdown cells (B, C). (D–F) Western blotting analysis was conducted to examine the protein expression of SMAD4 in PDAC cells. (G, H) The efficiency of SMAD4 knockdown in PANC-1 (G) and AsPC-1 cells (H) was detected by RT-qPCR. (I, J) Representative images of sphere-formation assays in PANC-1/KD and AsPC-1/KD cells transfected with siSMAD4-2. (K, L) The protein levels of SMAD4, KLF4, c-Myc, JNK, p-JNK, JUN and p-JUN in PANC-1/LINC00909-KD (K) and AsPC-1/LINC00909-KD cells (L) transfected with siSMAD4-2 were analyzed by western blotting. (M) PANC-1 cells transfected with EV or vector encoding LINC00909 were treated with actinomycin D (1 mg/ml) at the indicated time point. (N, O) PANC-1 or AsPC-1 cells transfected with siNC, siLINC00909-3, or siLINC00909-6 were treated with actinomycin D (1 mg/ml) at the indicated time point. Total RNA was extracted and analyzed by RT-qPCR to examine the relative levels of SMAD4 mRNA (M–O). All *P 
3). Novel polysaccharide identified from Cortinarius purpurascens demonstrated anti-fibrosis effects in cardiac fibroblasts. Food Bioscience, 2023 [IF=4.8]
4). Jiawei Maxing Shigan Tang alleviates radiation-induced lung injury via TGF-β1/Smad signaling pathway mediated by regulatory T cells. Journal of ethnopharmacology, 2024 (PubMed: 37944875) [IF=4.8]

Application: WB    Species: Human    Sample:

Fig. 1. Evaluation of the effects of CD4+CD25+ Tregs on TGF-β1/Smad pathway and the EMT process. (A) The release of TGF-β1 from Tregs was detected by ELISA. (B–F) Protein levels of Smad4, Vimentin, α-SMA, and E-cadherin in type II AECs co-cultured with Tregs supernatant were detected by Western blot. *P＜0.05, **P＜0.01, ***P＜0.001, n = 3.
5). Enhancements in flavor substances, mouthfeel characteristics and collagen synthesis in the muscle of sub-adult grass carp (Ctenopharyngodon Idella): Application of a dietary lysine nutrition strategy. Aquaculture, 2023 [IF=3.9]
6). Enhancement of flavor and healthcare substances, mouthfeel parameters and collagen synthesis in the muscle of on-growing grass carp (Ctenopharyngodon idella) fed with graded levels of glutamine. Aquaculture, 2020 [IF=3.9]
7). Astragaloside IV inhibits cell proliferation in vulvar squamous cell carcinoma through the TGF‐β/Smad signaling pathway. Dermatologic Therapy, 2019 (PubMed: 30536730) [IF=3.7]

Application: WB    Species: human    Sample: SW962 cells

Figure 3. |AS-Ⅳ reverses TGF-β/Smad signaling abnormalities and inhibits TGF-β1-mediated cell proliferation and anti-apoptosis in SW962 cells. SW962 cells were treated with TGF-β1 (10 ng/ml) alone or in combination with AS-Ⅳ (800 μg/ml) for 24 h and 48 h, respectively.Western blot assays evaluated the protein levels of TGF-βRI, TGF-βRII,Smad2/3, p-Smad2/3 and Smad4 (A,B).
8). Comprehensive upstream and downstream regulatory analyses identify miR-675-3p as a potential prognostic biomarker in melanoma. Human Cell, 2021 (PubMed: 33400243) [IF=3.4]

Application: WB    Species: Human    Sample: melanoma A375 cells

Fig. 7 miR-675-3p may promote cell proliferation, regulate the cell cycle, and activate the TGF-β, HIF-1 signaling pathways. a miR- 675-3p facilitated the G0/G1–G2/M transition in human melanoma A375 cells, b miR-675-3p promoted cell proliferation. c The TGFβ2, Smad2/3, Smad4, and HIF1A protein levels in the TGF-β/SMAD and HIF-1 signaling pathways were signifcantly higher, while TGF β1 was decreased relative to the negative control group
9). MMP13 as an effective target of an active trifluoromethyl quinazoline compound against osteosarcoma. Toxicology and applied pharmacology, 2025 (PubMed: 39674349) [IF=3.3]
10). Huangqi decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathway. BMC complementary medicine and therapies, 2023 (PubMed: 37365530) [IF=3.3]

Application: WB    Species: Mouse    Sample: kidneys

Fig. 6 HQD downregulated the expression of the Smad1 protein in the kidneys of db/db mice. A. The protein expression levels of P-Smad1/5/9, Smad1 and Smad4 in the kidney cortex by Western blot assay. B. Statistical results of the expression level of Smad1, Values are mean ± SEM (n = 3), *P 
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