GATA4 Antibody - #AF5245

Figure 4 HIPK1 knockdown inhibits pathological hypertrophy of cardiomyocytes through inactivation of the CREB-C/EBPβ axis. a) qRT-PCR for CREB in NRCMs transfected with OE-CREB plasmid or control vector (n = 6). b) Immunofluorescent staining for α-actinin in NRCMs treated with sh-HIPK1 lentivirus and/or OE-CREB plasmid (n = 6). Scale bar = 100 µm. c) qRT-PCR for C/EBPβ in NRCMs transfected with OE-C/EBPβ plasmid or control vector (n = 5). d) Immunofluorescent staining for α-actinin in NRCMs treated with sh-HIPK1 lentivirus and/or OE-C/EBPβ plasmid (n = 6). Scale bar = 100 µm. e) H9C2 cell lysates were incubated with C/EBPβ antibody, and co-immunoprecipitation of C/EBPβ with NFAT3 and GATA4 was evaluated by Western blot. f) qRT-PCR for genes associated with pathological cardiac hypertrophy in NRCMs (n = 6). g,h) Western blot for CREB phosphorylation and C/EBPβ expressions in heart tissues from WT and HIPK1 KO mice with g) sham or h) TAC operations (n = 3). i,j) qRT-PCR for C/EBPβ expression in heart tissues from WT and HIPK1 KO mice with i) sham or j) TAC operations (n = 5–6). Data between two groups were compared by independent-sample two-tailed Student's t-test. Data among four groups were compared by two-way ANOVA test followed by Tukey post hoc test. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Fig 7. |Effects of miR-20b on the BMP signaling pathway, as assessed by by western blotting. a): in the miR-20b overexpression group; b): in miR-20b silenced group. (n = 3, *: P<0.05, **: P<0.01, ***: P<0.001)

Figure 8 Effects of AMO-3568 on the protein expression levels of RPBJ (A, B), GATA6 (A, C) and GATA4 (A, D) were measured by Western blot assay. n = 4 for per group. Data were analyzed by one-way ANOVA followed by the Tukey’s test. *p < 0.05, versus the sham group, #p < 0.05 versus the SCII group.

A. Synaptophysin在肿瘤组织胞质中呈阳性表达，且阳性区域广泛, bar=200 μm；B. Synaptophysin在肿瘤组织胞质中呈阳性表达(黑色箭头)，bar=100 μm；C.S-100在肿瘤细胞中表达为少量细胞阳性反应(黑色箭头)，bar=100 μm；D. GATA4在肿瘤组织细胞核中表达为阳性反应(黑色箭头)，bar=100 μm；E. Pancytokeratin在肿瘤组织中表达为阴性反应，bar=200 μm；F. Vimentin在肿瘤组织中表达为阴性反应，bar=200 μm A. Synaptophysin was positively expressed in the cytoplasm of tumor tissues, and the positive region was extensive bar=200 μm; B. Synaptophysin was positively expressed in the cytoplasm of tumor tissue (black arrow), bar=100 μm; C. S-100 was expressed as a small amount of cell positive reaction in tumor cells (black arrow), bar=100 μm; D. GATA4 was positively expressed in the nucleus of tumor tissues (black arrow), bar=100 μm; E. Pancytokeratin was negatively expressed in tumor tissues, bar=200 μm; F. Vimentin was negative in tumor tissue, bar=200 μm 图 3 免疫组化结果 Fig. 3 Immunohistochemical results