(28)
(24)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5239, RRID:AB_2837725.
展开/折叠
CADH2_HUMAN; Cadherin 2; Cadherin 2 N cadherin neuronal; Cadherin 2 type 1; Cadherin 2 type 1 N cadherin neuronal; Cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin2; Calcium dependent adhesion protein neuronal; CD325; CD325 antigen; CDH2; CDHN; CDw325; CDw325 antigen; N cadherin 1; N-cadherin; NCAD; Neural cadherin; OTTHUMP00000066304; OTTHUMP00000067378;
抗原和靶标
- P19022 CADH2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MCRIAGALRTLLPLLAALLQASVEASGEIALCKTGFPEDVYSAVLSKDVHEGQPLLNVKFSNCNGKRKVQYESSEPADFKVDEDGMVYAVRSFPLSSEHAKFLIYAQDKETQEKWQVAVKLSLKPTLTEESVKESAEVEEIVFPRQFSKHSGHLQRQKRDWVIPPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGPGADQPPTGIFIINPISGQLSVTKPLDREQIARFHLRAHAVDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGSKPGTYVMTVTAIDADDPNALNGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQATDMEGNPTYGLSNTATAVITVTDVNDNPPEFTAMTFYGEVPENRVDIIVANLTVTDKDQPHTPAWNAVYRISGGDPTGRFAIQTDPNSNDGLVTVVKPIDFETNRMFVLTVAAENQVPLAKGIQHPPQSTATVSVTVIDVNENPYFAPNPKIIRQEEGLHAGTMLTTFTAQDPDRYMQQNIRYTKLSDPANWLKIDPVNGQITTIAVLDRESPNVKNNIYNATFLASDNGIPPMSGTGTLQIYLLDINDNAPQVLPQEAETCETPDPNSINITALDYDIDPNAGPFAFDLPLSPVTIKRNWTITRLNGDFAQLNLKIKFLEAGIYEVPIIITDSGNPPKSNISILRVKVCQCDSNGDCTDVDRIVGAGLGTGAIIAILLCIIILLILVLMFVVWMKRRDKERQAKQLLIDPEDDVRDNILKYDEEGGGEEDQDYDLSQLQQPDTVEPDAIKPVGIRRMDERPIHAEPQYPVRSAAPHPGDIGDFINEGLKAADNDPTAPPYDSLLVFDYEGSGSTAGSLSSLNSSSSGGEQDYDYLNDWGPRFKKLADMYGGGDD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P19022 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S61 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| N273 | N-Glycosylation | Uniprot | |
| N402 | N-Glycosylation | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| T454 | Phosphorylation | Uniprot | |
| Y496 | Phosphorylation | Uniprot | |
| K536 | Ubiquitination | Uniprot | |
| N572 | N-Glycosylation | Uniprot | |
| K667 | Ubiquitination | Uniprot | |
| T683 | Phosphorylation | Uniprot | |
| S685 | Phosphorylation | Uniprot | |
| N692 | N-Glycosylation | Uniprot | |
| K756 | Ubiquitination | Uniprot | |
| K772 | Ubiquitination | Uniprot | |
| Y773 | Phosphorylation | Uniprot | |
| Y785 | Phosphorylation | Uniprot | |
| S788 | Phosphorylation | Uniprot | |
| K802 | Ubiquitination | Uniprot | |
| Y820 | Phosphorylation | P12931 (SRC) | Uniprot |
| S824 | Phosphorylation | Uniprot | |
| Y852 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y860 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y884 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y886 | Phosphorylation | P12931 (SRC) | Uniprot |
研究背景
Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).
May be phosphorylated by OBSCN.
Cell membrane>Single-pass type I membrane protein. Cell membrane>Sarcolemma. Cell junction. Cell surface.
Note: Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes.
Homodimer (via extracellular region). Can also form heterodimers with other cadherins (via extracellular region). Dimerization occurs in trans, i.e. with a cadherin chain from another cell (By similarity). Interacts with CDCP1. Interacts with PCDH8; this complex may also include TAOK2 (By similarity). The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway (By similarity). Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. May interact with OBSCN (via protein kinase domain 2) (By similarity).
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. Calcium-binding sites are occupied sequentially in the order of site 3, then site 2 and site 1.
研究领域
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).
文献引用
Application: WB Species: Human Sample: 5-8 F and HNE1 cells
Application: WB Species: Human Sample: BMSCs
Application: WB Species: rat Sample: FLSs
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