CD83 Antibody - #AF5233

Figure 12. (A,B) NOD2 and CD83 contents in Y79 tumors after different treatments. (C) IFN-γ and MCP-1 levels in Y79 tumors after different treatments. (D) H&E staining in major organs (heart, liver, spleen, lung, and kidney) of all groups after treatments. The scale bars are 50 μm. (n = 5, **p < 0.01).

Figure 4. Subpopulation analysis of M1-polarization-like microglia clusters. (A) Pseudotime plot (from monocle3) shows the differentiation trajectory between Mic_M1L1 and Mic_M1L2 cells. (B) Line plot of the fraction of cells in Mic_M1L1 and Mic_M1L2 subpopulations over time. (C) Expression of the ten genes with the highest Moran’s I score alongside the pseudotime trajectory. (D) Violin plot of the functional pathways revealed by gene set variation analysis (GSVA) in the Mic_M1L1 and Mic_M1L2 subpopulations. (E) Volcano plot displays the differentially expressed genes between Mic_M1L1 and Mic_M1L2 cells. Red dots represent the upregulated genes in the Mic_M1L1 cluster compared with the Mic_M1L2 cluster and blue dots represent the downregulated. (F) Relative activity of the highly expressed transcription factors (TF) in Mic_M1L2 cells compared with Mic_M1L1 cells. (G) Double immunofluorescence staining of Mic_M1L1 cells at each sampling time. Coronal brain sections are all stained with anti-Iba1(green, representing microglia), Nfkbiz (red, the marker of Mic_M1L1 cells), and DAPI (blue, representing cell nuclei) antibodies (N=3). The yellow bar represents 100μm. The arrows point to the cells simultaneously marked by Iba1 and Nfkbiz. (H) Double immunofluorescence staining of Mic_M1L2 cells at each sampling time. Coronal brain sections are all stained with anti-Iba1(green, representing microglia), Cd83 (red, the marker of Mic_M1L2 cells), and DAPI (blue, representing cell nuclei) antibodies (N=3). The yellow bar represents 100μm. The arrows point to the cells simultaneously marked by Iba1 and Cd83. (I) Bar plot of the ratio of Nfkbiz+Iba1+ cells (green plus red) compared with all DAPI+ cells in a 500μm2 area surrounding the infarction core (N=6). (J) Bar plot of the ratio of Cd83+Iba1+ cells (green plus red) compared with all DAPI+ cells in a 500μm2 area surrounding the infarction core (N=6).