产品: AQP1 抗体
货号: AF5231
描述: Rabbit polyclonal antibody to AQP1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Horse, Dog
分子量: 29 kDa.; 29kD(Calculated).
蛋白号: P29972
RRID: AB_2837717

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Horse(80%), Dog(80%)
克隆:
Polyclonal
特异性:
AQP1 Antibody detects endogenous levels of total AQP1.
RRID:
AB_2837717
引用格式: Affinity Biosciences Cat# AF5231, RRID:AB_2837717.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AQP 1; AQP CHIP; AQP-1; AQP1; AQP1_HUMAN; aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group); aquaporin 1 (Colton blood group); Aquaporin CHIP; Aquaporin-1; Aquaporin-CHIP; Aquaporin1; Channel forming integral protein 28kDa; Channel like integral membrane protein 28 kDa; CHIP 28; CHIP28; CO; Colton blood group; Growth factor induced delayed early response protein; MGC26324; Urine water channel; Water channel protein CHIP 29; Water channel protein CHIP29; Water channel protein for red blood cells and kidney proximal tubule;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P29972 AQP1_HUMAN:

Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.

描述:
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
序列:
MASEFKKKLFWRAVVAEFLATTLFVFISIGSALGFKYPVGNNQTAVQDNVKVSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISIFRALMYIIAQCVGAIVATAILSGITSSLTGNSLGRNDLADGVNSGQGLGIEIIGTLQLVLCVLATTDRRRRDLGGSAPLAIGLSVALGHLLAIDYTGCGINPARSFGSAVITHNFSNHWIFWVGPFIGGALAVLIYDFILAPRSSDLTDRVKVWTSGQVEEYDLDADDINSRVEMKPK

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
80
Dog
80
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P29972 作为底物

Site PTM Type Enzyme
T157 Phosphorylation P17252 (PRKCA)
S236 O-Glycosylation
T239 Phosphorylation P17252 (PRKCA)
Y253 Phosphorylation
S262 Phosphorylation

研究背景

功能:

Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.

亚基结构:

Homotetramer. Interacts with EPHB2; involved in endolymph production in the inner ear (By similarity). Identified in a complex with STOM.

蛋白家族:

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Belongs to the MIP/aquaporin (TC 1.A.8) family.

研究领域

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.

文献引用

1). Synergistic effect of constituent drugs of Baibutang on improving Yin-deficiency pulmonary fibrosis in rats. JOURNAL OF ETHNOPHARMACOLOGY (PubMed: 36535334) [IF=5.4]

2). MMP‐7 affects peritoneal ultrafiltration associated with elevated aquaporin‐1 expression via MAPK/ERK pathway in peritoneal mesothelial cells. Journal of Cellular and Molecular Medicine (PubMed: 34117704) [IF=5.3]

Application: IF/ICC    Species: Human    Sample: peritoneal mesothelial cells

FIGURE 4 Ectopic expression of MMP-7 enhanced AQP-1 expression and then altered cell volume in peritoneal mesothelial cells. HMrSV5 cells were infected with lentivirus expressing MMP-7. (a, b) The expression of MMP-7 in the lentivirus-transfected cells was determined by quantitative RT-PCR (a) and Western blotting analysis (b). (c) The MMP-7-overexpressing cells (Lv-MMP-7) and control cells (Lv-NC) were incubated in NS or dialysate with 4.25% glucose for 1 minute. The cell diameter of peritoneal mesothelial cells was evaluated by the Scepter 2.0 cell counter. The changes of cell diameter after glucose stimulation were calculated. (d-f) The mRNA level of AQP-1 was detected by quantitative RT-PCR (d), and the protein expression of AQP-1 was assessed by Western blotting assay (e). Besides, the cells were permeabilized by Triton-X 100 and immunofluorescence assay was used to detect the expression of AQP-1 (f). (g, h) The cells were treated with the indicated 50 ng/ml MMP-7 for 12 hours. The expression of AQP-1 in the HMrSV5 cell membrane was evaluated by Western blotting (g) and immunofluorescence assay (h), HMrSV5 cells were transfected with AQP-1-targeting siRNAs for 36 hours. The expression level of AQP-1 protein was assayed by immunoblotting. The #3 siRNA against AQP-1 was selected for the further analysis. (I) After transfecting with AQP-1-targeting siRNAs for 36 hours, HMrSV5 cells were incubated with 100 ng/ml MMP-7 for another 24 hours. Cell diameter of the peritoneal mesothelial cells was evaluated by the Scepter 2.0 cell counter (J). *P < 0.05, **P < 0.01, one of the three independent experiments is shown

Application: WB    Species: Human    Sample: peritoneal mesothelial cells

FIGURE 4 Ectopic expression of MMP-7 enhanced AQP-1 expression and then altered cell volume in peritoneal mesothelial cells. HMrSV5 cells were infected with lentivirus expressing MMP-7. (a, b) The expression of MMP-7 in the lentivirus-transfected cells was determined by quantitative RT-PCR (a) and Western blotting analysis (b). (c) The MMP-7-overexpressing cells (Lv-MMP-7) and control cells (Lv-NC) were incubated in NS or dialysate with 4.25% glucose for 1 minute. The cell diameter of peritoneal mesothelial cells was evaluated by the Scepter 2.0 cell counter. The changes of cell diameter after glucose stimulation were calculated. (d-f) The mRNA level of AQP-1 was detected by quantitative RT-PCR (d), and the protein expression of AQP-1 was assessed by Western blotting assay (e). Besides, the cells were permeabilized by Triton-X 100 and immunofluorescence assay was used to detect the expression of AQP-1 (f). (g, h) The cells were treated with the indicated 50 ng/ml MMP-7 for 12 hours. The expression of AQP-1 in the HMrSV5 cell membrane was evaluated by Western blotting (g) and immunofluorescence assay (h), HMrSV5 cells were transfected with AQP-1-targeting siRNAs for 36 hours. The expression level of AQP-1 protein was assayed by immunoblotting. The #3 siRNA against AQP-1 was selected for the further analysis. (I) After transfecting with AQP-1-targeting siRNAs for 36 hours, HMrSV5 cells were incubated with 100 ng/ml MMP-7 for another 24 hours. Cell diameter of the peritoneal mesothelial cells was evaluated by the Scepter 2.0 cell counter (J). *P < 0.05, **P < 0.01, one of the three independent experiments is shown

3). Combination of pseudoephedrine and emodin ameliorates LPS-induced acute lung injury by regulating macrophage M1/M2 polarization through the VIP/cAMP/PKA pathway. Chinese Medicine (PubMed: 35123524) [IF=4.9]

Application: WB    Species: Rat    Sample: lung tissues 

Fig. 1 Effect of Pseudoephedrine + emodin on pathological changes of lung tissues (×200), expression of AQP‐1, AQP‐5 in LPS‐induced ALI rats. A The chemical structure of Pseudoephedrine and Emodin. B Stained with hematoxylin and eosin. C The lung injury score was determined following a five-point scale from 0 to 4 as follows: 0, l, 2, 3, and 4 represent no damage, mild damage, moderate damage, severe damage, and very severe damage, respectively. Representative histological sections of the lungs were stained with hematoxylin and eosin (H & E staining, magnification ×200). D–F Western blot analysis was performed to detect AQP‐1 and AQP‐5 protein expression. K The dry/wet weight ratio and lung weight/body weight ratio were used to reflect the pulmonary edema. All data are expressed as mean ± S.D. (n = 5). ##p < 0.01, ###p < 0.001 vs. control group. *p < 0.05, **p < 0.01, ***p < 0.001 vs. LPS alone group. +p < 0.05, +++p < 0.001 vs. combined treatment group (5 + 20 mg/kg)

4). MiR-3194-3p Inhibits Breast Cancer Progression by Targeting Aquaporin1. Frontiers in Oncology (PubMed: 32903818) [IF=4.7]

Application: WB    Species: human    Sample: BC tissues and cell lines

FIGURE 1 | The miR-3194-3p and AQP1 expression levels in BC tissues and cell lines. Comparing differences in the expression levels of miR-3194-3p between(A) BC and adjacent non-tumor tissues (n = 30); (B) BC cell lines and the control breast epithelial cells (MCF-10A); differences in AQP1 mRNA level between (C) BC and adjacent non-tumor tissue; (D) BC cell lines and MCF-10A; differences in AQP1 protein level between (E) BC cell lines and MCF-10A.

5). Hyperglycemia induces corneal endothelial dysfunction through attenuating mitophagy. EXPERIMENTAL EYE RESEARCH (PubMed: 34951999) [IF=3.4]

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