Cytochrome P450 19A1 Antibody - #AF5229

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产品: Cytochrome P450 19A1 抗体
货号: AF5229
描述: Rabbit polyclonal antibody to Cytochrome P450 19A1
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Sheep, Rabbit, Dog
蛋白号: P11511
RRID: AB_2837715

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Cytochrome P450 19A1 Antibody detects endogenous levels of total Cytochrome P450 19A1.
RRID:
AB_2837715
引用格式: Affinity Biosciences Cat# AF5229, RRID:AB_2837715.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ARO; ARO1; Aromatase; CP19A_HUMAN; CPV1; CYAR; CYP19; Cyp19a1; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Cytochrome P450, family 19, subfamily A, polypeptide 1; Cytochrome P450, subfamily XIX (aromatization of androgens); Estrogen synthase; Estrogen synthetase; Flavoprotein linked monooxygenase; MGC104309; Microsomal monooxygenase; OTTHUMP00000162543; OTTHUMP00000198350; P 450AROM;

抗原和靶标

免疫原:

A synthesized peptide derived from human Cytochrome P450 19A1, corresponding to a region within the internal amino acids.

基因/基因ID:
CYP19A1(1588)
描述:
Defects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity. Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency.

研究领域

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

文献引用

1). CIRBP Enhances the Function of Yak Cumulus Cells by Activating AMPK/mTOR-Mediated Mitophagy. Biomolecules, 2025 (PubMed: 40563401) [IF=5.5]
2). CIRBP Increases the synthesis and secretion of steroid hormones by in yak granulaso cells. The Journal of steroid biochemistry and molecular biology, 2024 (PubMed: 38143009) [IF=2.7]
3). Exosomes Derived from Yak Follicular Fluid Increase 2-Hydroxyestradiol Secretion by Activating Autophagy in Cumulus Cells. Animals, 2022 (PubMed: 36428401) [IF=2.7]

Application: IF/ICC    Species: Yak    Sample: YCCs

Figure 5. Yak follicular fluid exosomes could increase 2-OHE2 secretion in YCCs. (A) Immunofluorescence staining of CYP19A1 and CYP1A1 proteins in YCCs. Scale bar represents 50 μm. (B) Expression of 2-OHE2 secretion-related genes CYP17A1 (B1), CYP19A1 (B2), CYP1A1 (B3) and CYP1B1 (B4) in YCCs using the qPCR assay. (C) Western blot analysis of the protein expression in YCCs (C: Control, E: Yak follicular fluid exosomes) (See Figure S2). (D) ELISA kit analyses of the concentrations of estradiol (pg/mL) in the cell supernatant. (E) Quantitative Western blot results for CYP17A1 (E1), CYP19A1 (E2), CYP1A1 (E3) and CYP1B1 (E4). Data are expressed as the mean ± SD. n = 6. NS means no significant difference. * p < 0.05 and ** p < 0.01 indicate significant differences between the yak follicular fluid exosome treatments and the control.

Application: WB    Species: Yak    Sample: YCCs

Figure 5. Yak follicular fluid exosomes could increase 2-OHE2 secretion in YCCs. (A) Immunofluorescence staining of CYP19A1 and CYP1A1 proteins in YCCs. Scale bar represents 50 μm. (B) Expression of 2-OHE2 secretion-related genes CYP17A1 (B1), CYP19A1 (B2), CYP1A1 (B3) and CYP1B1 (B4) in YCCs using the qPCR assay. (C) Western blot analysis of the protein expression in YCCs (C: Control, E: Yak follicular fluid exosomes) (See Figure S2). (D) ELISA kit analyses of the concentrations of estradiol (pg/mL) in the cell supernatant. (E) Quantitative Western blot results for CYP17A1 (E1), CYP19A1 (E2), CYP1A1 (E3) and CYP1B1 (E4). Data are expressed as the mean ± SD. n = 6. NS means no significant difference. * p < 0.05 and ** p < 0.01 indicate significant differences between the yak follicular fluid exosome treatments and the control.

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