Cytochrome P450 19A1 Antibody - #AF5229

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产品: Cytochrome P450 19A1 抗体
货号: AF5229
描述: Rabbit polyclonal antibody to Cytochrome P450 19A1
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Sheep, Rabbit, Dog
蛋白号: P11511
RRID: AB_2837715

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Cytochrome P450 19A1 Antibody detects endogenous levels of total Cytochrome P450 19A1.
RRID:
AB_2837715
引用格式: Affinity Biosciences Cat# AF5229, RRID:AB_2837715.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ARO; ARO1; Aromatase; CP19A_HUMAN; CPV1; CYAR; CYP19; Cyp19a1; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Cytochrome P450, family 19, subfamily A, polypeptide 1; Cytochrome P450, subfamily XIX (aromatization of androgens); Estrogen synthase; Estrogen synthetase; Flavoprotein linked monooxygenase; MGC104309; Microsomal monooxygenase; OTTHUMP00000162543; OTTHUMP00000198350; P 450AROM;

抗原和靶标

免疫原:

A synthesized peptide derived from human Cytochrome P450 19A1, corresponding to a region within the internal amino acids.

基因/基因ID:
CYP19A1(1588)
描述:
Defects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity. Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency.

研究领域

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

文献引用

1). CIRBP Enhances the Function of Yak Cumulus Cells by Activating AMPK/mTOR-Mediated Mitophagy. Biomolecules, 2025 (PubMed: 40563401) [IF=5.5]

Application: WB    Species: Yak    Sample: Yak Cumulus Cells

Figure 3. Overexpression of CIRBP improves YCC function. (A) Steroid-synthesis-related factors expression level after CIRBP overexpression, n = 4. (B) The mRNA expression level of cumulus-diffusion-related factors, n = 4. (C) The secretion of E2 and P4, n = 4. (D) E2 and P4 synthesis-related protein bands and analysis, n = 3. (E) Cumulus-diffusion-related protein bands and analysis, n = 3. (F) Immunofluorescence detection of cumulus-diffusion-related proteins. Values are mean ± SE, *: p < 0.05, **: p < 0.01, NS: no difference. Original Western blot images are provided in the Supplementary Materials.
2). Exosomes Derived from Yak Follicular Fluid Increase 2-Hydroxyestradiol Secretion by Activating Autophagy in Cumulus Cells. Animals, 2022 (PubMed: 36428401) [IF=2.7]

Application: IF/ICC    Species: Yak    Sample: YCCs

Figure 5. Yak follicular fluid exosomes could increase 2-OHE2 secretion in YCCs. (A) Immunofluorescence staining of CYP19A1 and CYP1A1 proteins in YCCs. Scale bar represents 50 μm. (B) Expression of 2-OHE2 secretion-related genes CYP17A1 (B1), CYP19A1 (B2), CYP1A1 (B3) and CYP1B1 (B4) in YCCs using the qPCR assay. (C) Western blot analysis of the protein expression in YCCs (C: Control, E: Yak follicular fluid exosomes) (See Figure S2). (D) ELISA kit analyses of the concentrations of estradiol (pg/mL) in the cell supernatant. (E) Quantitative Western blot results for CYP17A1 (E1), CYP19A1 (E2), CYP1A1 (E3) and CYP1B1 (E4). Data are expressed as the mean ± SD. n = 6. NS means no significant difference. * p < 0.05 and ** p < 0.01 indicate significant differences between the yak follicular fluid exosome treatments and the control.

Application: WB    Species: Yak    Sample: YCCs

Figure 5. Yak follicular fluid exosomes could increase 2-OHE2 secretion in YCCs. (A) Immunofluorescence staining of CYP19A1 and CYP1A1 proteins in YCCs. Scale bar represents 50 μm. (B) Expression of 2-OHE2 secretion-related genes CYP17A1 (B1), CYP19A1 (B2), CYP1A1 (B3) and CYP1B1 (B4) in YCCs using the qPCR assay. (C) Western blot analysis of the protein expression in YCCs (C: Control, E: Yak follicular fluid exosomes) (See Figure S2). (D) ELISA kit analyses of the concentrations of estradiol (pg/mL) in the cell supernatant. (E) Quantitative Western blot results for CYP17A1 (E1), CYP19A1 (E2), CYP1A1 (E3) and CYP1B1 (E4). Data are expressed as the mean ± SD. n = 6. NS means no significant difference. * p < 0.05 and ** p < 0.01 indicate significant differences between the yak follicular fluid exosome treatments and the control.
3). CIRBP Increases the synthesis and secretion of steroid hormones by in yak granulaso cells. The Journal of steroid biochemistry and molecular biology, 2024 (PubMed: 38143009) [IF=2.7]

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