Elastin Antibody - #AF5226

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产品: Elastin 抗体
货号: AF5226
描述: Rabbit polyclonal antibody to Elastin
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse
蛋白号: P15502
RRID: AB_2837712

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Polyclonal
特异性:
Elastin Antibody detects endogenous levels of total Elastin.
RRID:
AB_2837712
引用格式: Affinity Biosciences Cat# AF5226, RRID:AB_2837712.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Elastin; ELN; ELN_HUMAN; SVAS; Tropoelastin; WBS; WS;

抗原和靶标

免疫原:

A synthesized peptide derived from human Elastin, corresponding to a region within the internal amino acids.

基因/基因ID:
ELN(2006)
描述:
Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle

研究领域

· Organismal Systems > Digestive system > Protein digestion and absorption.

文献引用

1). Deficiency of Integrin β 4 Results in Increased Lung Tissue Stiffness and Responds to Substrate Stiffness via Modulating RhoA Activity. Frontiers in cell and developmental biology, 2022 (PubMed: 35309934) [IF=4.6]

Application: IHC    Species: Mouse    Sample:

FIGURE 3. The expression of collagens, elastin and LOXs between ITGB4f/f and ITGB4ccsp.cre mice. (A,B) Masson’s Trichrome stain image (a, b) and ImageJ Imaging Software-generated image (c, d) to calculate Area % of collagen (Red). Scale bars: 50 μm. (C,D) The mRNA expression levels of collagens, elastin and LOXs were detected by RT-PCR. (E) Immunostaining for LOXL4 in airway epithelium (a,b) and alveolar septa (c,d) in ITGB4f/fand ITGB4ccsp.crelungs. Scale bars: 50 μm for (a, b);20 μm for (c, d). (F) Immunostaining for COLVI in pulmonary alveoli (a–d) and in airway epithelium (e–h) in ITGB4f/f and ITGB4ccsp.crelungs. Scale bars: 200 μm for (a, b, e, f); 50 μm for (c, d, g, h). (G) Immunostaining for elastin in pulmonary alveoli. Scale bars: 20 μm. (n = 3 independent experiments, *p < 0.05, **p < 0.01). Data are presented as mean ± SD.

Application: IF/ICC    Species: Mouse    Sample:

FIGURE 4. The expression of LOXL4,ColⅥ and elastin between ITGB4f/f and. ITGB4ccsp.cre mice (A) Immunofluorescence images of Col VI (green) and LOXL4 (pink) in the airway epithelium (a, b) and the Col VI (green) and elastin (red) in alveoli (c, d). Scale bars: 50 μm. (B) Immunofluorescence images of elastin in the in alveoli. Scale bars: 20 μm. Lung elastin accumulation (arrows) was distinctly greater in the ITGB4ccsp.cre lungs.
2). Pathological changes and expression of lysine oxidases and matrix metalloproteinases‐1,‐2, and‐3 in ligaments of patients with haemophilic arthritis. HAEMOPHILIA, 2022 (PubMed: 34697874) [IF=3.0]

Application: WB    Species: human    Sample: cruciate ligaments

Figure 6.|CollagenI and elastin expression levels in the cruciate ligaments of patients with OA or HA
3). Hydrogel Derived from Decellularized Porcine Small Intestinal Submucosa as a Physical Shielding Repaired the Gut Epithelial Barrier of Murine Ulcerative Colitis. Advanced Functional Materials, 2024

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