NCF1/p47-phox Antibody - #AF5220

Fig. 5 Effects of ELA on DOCA/salt-induced NADPH oxidase formation and activation. a mRNA levels of p22phox, gp91phox, p47phox, and p67phox subunits of NADPH oxidase in the renal cortex. b mRNA levels of p22phox, gp91phox, p47phox, and p67phox subunits of NADPH oxidase in the renal medulla. c Representative immunoblots of p22phox and p47phox and summarized intensities of blots. d Intracellular NADP+/NADPH in renal cortex and medulla. N = 6 per group. *P < 0.05 versus Ctrl group; #P < 0.05 versus DOCA/salt group.

Figure 4. Stachydrine hydrochloride (STA) decreases NOX2 expression and membrane ectopic expression of p67 and p47 in neonatal rat cardiomyocytes cultured with phenylephrine. (A) Quantitative statistical plots of NOX2 and the expression of its regulatory subunits in NRCMs and grayscale values of protein bands after stimulation with PE and STA for 48 h. (B) Images representative of the colocalization of WGA and p67phox and p47phox; visualization of p67phox and p47phox using Alexa Fluor 488-conjugated stain (green), whereas staining of WGA using Alexa Fluor 555 (red), ×400. * vs. control group, p < 0.05; # vs. H2O2 group, p < 0.05; Wihte arrow: A local magnification view of cell membrane. NRCMs: neonatal rat cardiomyocytes; PE: phenylephrine.

Fig. 8.TRPV4 activation promotes ROS production by triggering the membrane translocation of gp47phox. Neutrophils from mouse bone marrow were pretreated for 30 min with the indicated concentrations of GSK219 or Ca2+-free medium, followed by stimulation with GSK101 (300 nM) for 15 min. The preparation of membrane and cytosolic proteins is described in the Materials and methods section. Representative blots (A) and the histogram (B) of gp47phox expression in the cytosolic and membrane (n = 6 to 7 per group). (C–E) Representative images and quantification of immunofluorescence staining of gp91phox (green) and gp47phox (red) in WT neutrophils. Nuclei were stained with DAPI (blue). Scale bar = 20 μm, n = 3 per group. Statistical analyses: 2-tailed unpaired Student's t-test and 1-way ANOVA with Bonferroni's post hoc test; ***P < 0.001vs Ctrl; ##P < 0.01, ###P < 0.001 vs GSK101; NS, not significant. Data are expressed as the mean ± SEM.

Fig. 8.TRPV4 activation promotes ROS production by triggering the membrane translocation of gp47phox. Neutrophils from mouse bone marrow were pretreated for 30 min with the indicated concentrations of GSK219 or Ca2+-free medium, followed by stimulation with GSK101 (300 nM) for 15 min. The preparation of membrane and cytosolic proteins is described in the Materials and methods section. Representative blots (A) and the histogram (B) of gp47phox expression in the cytosolic and membrane (n = 6 to 7 per group). (C–E) Representative images and quantification of immunofluorescence staining of gp91phox (green) and gp47phox (red) in WT neutrophils. Nuclei were stained with DAPI (blue). Scale bar = 20 μm, n = 3 per group. Statistical analyses: 2-tailed unpaired Student's t-test and 1-way ANOVA with Bonferroni's post hoc test; ***P < 0.001vs Ctrl; ##P < 0.01, ###P < 0.001 vs GSK101; NS, not significant. Data are expressed as the mean ± SEM.