TOMM20 Antibody - #AF5206

Fig. 4 |Inhibition of mitophagy aggravates hepatic fibrosis in mice. a H&E staining of liver sections from mice; n = 6 per group; scale bar, 50 μm.b Sirius red staining of collagen in mouse liver sections; n = 6 per group; scale bar, 50 μm. c Representative western blots of TOM20, collagen I and α-SMA. Bar graph represents the mean ± SEM of three different experiments. *P < 0.05; **P < 0.01; and ***P < 0.001 vs. the indicated groups. d Serum levels of ALT and AST in the indicated mice. Bar graph represents the mean ± SEM; n = 6 per group. *P < 0.05 vs. The indicated groups

Fig. 4 Transferred mitochondria in MG/MΦ increased the number of mitochondria in hippocampus, and improved the morphology and structure of mitochondria in MG/MΦ. (A) Immunofluorescence analysis of mitochondria in hippocampal tissue. Green-stained cells are MG/MΦ, blue-stained regions are nuclei, and red arrows indicate the Mito-tracker Red CMXRos labeled exogenous mitochondria (400×, n = 3). (B) Immunofluorescence analysis of mitochondrial number. Blue-stained regions are nuclei, and red-stained regions are mitochondria in the hippocampus (400×, n = 3). (C) The mean gray value of TOMM20. (n = 3). (D) Morphology and structure of mitochondria in MG/MΦ were observed by electron microscopy. The red arrows indicate mitochondria; (scale bar 500 nm, n = 3); nsp > 0.05; *** p 

Fig. 3. AS-IV ameliorates mitochondrial damage induced by H2O2 combined with CCCP in L6 myoblasts. (A, B) Effects of AS-IV on intracellular ROS (bar = 100 µm). (C) Effects of AS-IV on ATP activities. (D) Effects of AS-IV on Tom 20 mRNA expression. (E) Effects of AS-IV on Tim 23 mRNA expression. (F) Effects of AS-IV on VDAC1 mRNA expression. (G) Effects of AS-IV on Tom 20 protein expression. (H) Effects of AS-IV on Tim 23 protein expression. (I) Effects of AS-IV on VDAC1 protein expression. ★P