SOD1 Antibody - #AF5198

Fig. 3. 6BQ NPs promote angiogenesis in vitro by modulating the HSP90/HIF-1α axis through MPTT, while inhibiting oxidative stress and inflammatory responses. A, B, C mRNA expression of HIF-1α, HSP90 and VEGF in HUVECs of every group was measured by RT-qPCR. D Western blotting of HIF-1α, HSP90 and VEGF expressions in HUVECs of the three groups. E Western blotting of HO-1 and SOD1 expressions in HUVECs of the three groups. F Western blotting of IL-6 expressions in HUVECs of the three groups. G The optical density values of HIF-1α, HSP90 and VEGF in HUVECs of the three groups. H The optical density values of HO-1 and SOD1 in HUVECs of the three groups. I The optical density values of IL-6 in HUVECs of the three groups. J Mean SOD activity of HUVECs in the three groups. K Mean MDA content of HUVECs in the three groups. Data are presented as mean ± SD, n = 6 per group. ns P > 0.05, **P 

Fig. 2 Chlorogenic acid-induced protection of brain post-hypoxic-ischemic brain injury via the down-regulation of expression of inflammatory and oxidative stress levels. a The IL-1 β level in brain tissue 24 h after HI brain injury measured by Elisa kit. ∗P < 0.05 vs. the sham group. ##P < 0.01 vs. the HI group. n=3. b The MDA level in brain tissue 24 h after HI brain injury by MDA kit. ∗∗∗P < 0.001 vs. the sham group. #P < 0.05 vs. the HI group. n=3. c The CAT level in brain tissue 24 h after HI brain injury by CAT kit. ∗∗∗P < 0.001 vs. the sham group. #P < 0.05 vs. the HI group. n=3. d Western blot detection of the protein levels of iNOS, SOD2/MnSOD, TNF-α, pre-IL-1β and mature- IL-1β 24h after HI injury. e–h Quantification of western blot data of iNOS, SOD2/MnSOD, TNF-α, and mature-IL-1 β. ∗∗P < 0.01 and ∗∗∗P < 0.001 vs. the sham group. #P < 0.05, ##P < 0.01 and ###P < 0.001 vs. the HI group. n = 3

Fig. 2 GA alleviates mitochondrial oxidative stress induced by excessive exercise in mice A-B Immunohistochemical analysis of SOD expression in skeletal muscle tissue from different groups of mice, with “_” indicating 100 μm, accompanied by quantitative histograms C Detection of ATP, GSH and GSSG levels in skeletal muscle tissue by ELISA D-E Western blot bands represent the expression levels of TOM20 and SOD proteins in different groups of mouse tissues, with histograms representing the quantitative data of each index standardized to β-actin F Detection of TOM20 and SOD mRNA levels in skeletal muscle tissue by RT-qPCR Values are expressed as the mean ± SD from three independent experiments (n = 3). * p 

FIGURE 7 Effect of MLG on some protein levels in ethanol-induced gastric lesions mice. Some representative western blot bands (A). Protein levels of SOD1/GAPDH (B), SOD2/GAPDH (C), iNOS/GAPDH (D), nNOS/GAPDH (E), eNOS/GAPDH (F), COX2/GAPDH (G), p38/GAPDH (H) in mice gastric tissues. SOD1, Superoxide dismutase one or Cu/Zn-superoxide dismutase; SOD2/Mn SOD, superoxide dismutase 2. Control: the group administered zero ethanol; Model: the group administered ethanol intragastrically (13.25 ml/kg BW); MLG-L: low-dose (5 g/kg body weight) MLG-treated group; MLG-M: medium-dose (10 g/kg body weight) MLG-treated group; MLG-H: high-dose (20 g/kg body weight) MLG-treated group; CBP: the group receiving Colloid Bismuth Pectin (57 mg/kg body weight). Each group’s data was expressed as mean ± standard deviation (SD), n = 6. By one-way analysis of variance (ANOVA) test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. each group. Whole page of western blot can be found in Supplementary Figures S1, S3, S7, S8, S10, S14, S15, respectively.