产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5197, RRID:AB_2837683.
展开/折叠
ACE 1; ACE; ACE T; ACE_HUMAN; ACE1; Angiotensin converting enzyme somatic isoform; Angiotensin converting enzyme testis specific isoform; Angiotensin I converting enzyme 1; Angiotensin I converting enzyme; Angiotensin I converting enzyme peptidyl dipeptidase A 1; angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript; Angiotensin-converting enzyme; Carboxycathepsin; CD 143; CD143; CD143 antigen; DCP 1; DCP; DCP1; Dipeptidyl carboxypeptidase 1; Dipeptidyl carboxypeptidase I; Kininase II; MGC26566; MVCD3; Peptidase P; Peptidyl dipeptidase A; soluble form; Testicular ECA;
抗原和靶标
Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
- P12821 ACE_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGAASGRRGPGLLLPLPLLLLLPPQPALALDPGLQPGNFSADEAGAQLFAQSYNSSAEQVLFQSVAASWAHDTNITAENARRQEEAALLSQEFAEAWGQKAKELYEPIWQNFTDPQLRRIIGAVRTLGSANLPLAKRQQYNALLSNMSRIYSTAKVCLPNKTATCWSLDPDLTNILASSRSYAMLLFAWEGWHNAAGIPLKPLYEDFTALSNEAYKQDGFTDTGAYWRSWYNSPTFEDDLEHLYQQLEPLYLNLHAFVRRALHRRYGDRYINLRGPIPAHLLGDMWAQSWENIYDMVVPFPDKPNLDVTSTMLQQGWNATHMFRVAEEFFTSLELSPMPPEFWEGSMLEKPADGREVVCHASAWDFYNRKDFRIKQCTRVTMDQLSTVHHEMGHIQYYLQYKDLPVSLRRGANPGFHEAIGDVLALSVSTPEHLHKIGLLDRVTNDTESDINYLLKMALEKIAFLPFGYLVDQWRWGVFSGRTPPSRYNFDWWYLRTKYQGICPPVTRNETHFDAGAKFHVPNVTPYIRYFVSFVLQFQFHEALCKEAGYEGPLHQCDIYRSTKAGAKLRKVLQAGSSRPWQEVLKDMVGLDALDAQPLLKYFQPVTQWLQEQNQQNGEVLGWPEYQWHPPLPDNYPEGIDLVTDEAEASKFVEEYDRTSQVVWNEYAEANWNYNTNITTETSKILLQKNMQIANHTLKYGTQARKFDVNQLQNTTIKRIIKKVQDLERAALPAQELEEYNKILLDMETTYSVATVCHPNGSCLQLEPDLTNVMATSRKYEDLLWAWEGWRDKAGRAILQFYPKYVELINQAARLNGYVDAGDSWRSMYETPSLEQDLERLFQELQPLYLNLHAYVRRALHRHYGAQHINLEGPIPAHLLGNMWAQTWSNIYDLVVPFPSAPSMDTTEAMLKQGWTPRRMFKEADDFFTSLGLLPVPPEFWNKSMLEKPTDGREVVCHASAWDFYNGKDFRIKQCTTVNLEDLVVAHHEMGHIQYFMQYKDLPVALREGANPGFHEAIGDVLALSVSTPKHLHSLNLLSSEGGSDEHDINFLMKMALDKIAFIPFSYLVDQWRWRVFDGSITKENYNQEWWSLRLKYQGLCPPVPRTQGDFDPGAKFHIPSSVPYIRYFVSFIIQFQFHEALCQAAGHTGPLHKCDIYQSKEAGQRLATAMKLGFSRPWPEAMQLITGQPNMSASAMLSYFKPLLDWLRTENELHGEKLGWPQYNWTPNSARSEGPLPDSGRVSFLGLDLDAQQARVGQWLLLFLGIALLVATLGLSQRLFSIRHRSLHRHSHGPQFGSEVELRHS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P12821 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N38 | N-Glycosylation | Uniprot | |
| N54 | N-Glycosylation | Uniprot | |
| N74 | N-Glycosylation | Uniprot | |
| N111 | N-Glycosylation | Uniprot | |
| K136 | Acetylation | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| N146 | N-Glycosylation | Uniprot | |
| Y151 | Phosphorylation | Uniprot | |
| N318 | N-Glycosylation | Uniprot | |
| N445 | N-Glycosylation | Uniprot | |
| N509 | N-Glycosylation | Uniprot | |
| N695 | N-Glycosylation | Uniprot | |
| T697 | Phosphorylation | Uniprot | |
| N714 | N-Glycosylation | Uniprot | |
| T716 | Phosphorylation | Uniprot | |
| K718 | Ubiquitination | Uniprot | |
| N760 | N-Glycosylation | Uniprot | |
| R814 | Methylation | Uniprot | |
| S824 | Phosphorylation | Uniprot | |
| R826 | Methylation | Uniprot | |
| N942 | N-Glycosylation | Uniprot | |
| N1191 | N-Glycosylation | Uniprot | |
| S1299 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
研究背景
Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
Phosphorylated by CK2 on Ser-1299; which allows membrane retention.
Secreted.
Cell membrane>Single-pass type I membrane protein. Cytoplasm.
Note: Detected in both cell membrane and cytoplasm in neurons.
Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
Belongs to the peptidase M2 family.
研究领域
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Organismal Systems > Endocrine system > Renin-angiotensin system. (View pathway)
· Organismal Systems > Endocrine system > Renin secretion.
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