ACE1 Antibody - #AF5197

Fig. 2. GTS-21 regulated the ACE/ACE2 ratio and glycocalyx shedding in LPS-ALI mice A-D ELISA kits were used to quantify the concentrations of major renin-angiotensin system (RAS) regulating enzymes (ACE, Ang II, ACE2, and Ang 1-7) in the lung tissue (n = 6 per group). E Protein levels of important RAS-regulating enzymes (ACE, AT1R, ACE2, and MasR) (n = 3 per group). The relative protein levels are displayed. F Levels of NF-κB phosphoproteins (p-P65 and p-IκB) (n = 3 per group). The relative protein levels are displayed. The immunofluorescence of HS, SDC-1, and surfactant protein D in the lungs is shown in G in representative images and densitometry (scar bar, 20 mm, n = 3 per group). H Evans Blue staining tested lung permeability (n = 3 for each group). Using ELISA, we assessed the BALF concentrations of HS I and HPA J; SP-D in serum K and BALF L (n = 5 for each group). M Relative protein levels of SDC-1, HPA, EXT-1, and SP-D in The lung tissues (n = 3 for each group). All data were shown as mean ± SD.

Figure 2. ACE knockdown diminishes the sensitization of NPC cells to IR by reducing the ROS level. (A) The mRNA level of ACE was measured by RT-qPCR after ACE knockdown. (B) The protein level of ACE was detected by WB after ACE knockdown. (C–F) The viability of NPC cells was measured by CCK8 assay. ACE knockdown via siRNA treatment and irradiation with a dose of 4 Gy radiation shows sustained growth promotion, although ACE knockdown alone does not increase the proliferation of these cells. (G–J) The mean fluorescence intensity of DCFH-DA (DCF) was measured using flow cytometry to determine the level of intracellular ROS. Green: fluorescence histogram on the left side of the scale line; red: fluorescence histogram on the right side of the scale line. ROS levels were reduced after ACE knockdown compared to controls in both the IR and non-IR groups. (K) Mitochondrial membrane potential changes measured by laser confocal microscopy (JC-1 staining). ACE knockdown reverses radiotherapy-induced decrease in mitochondrial membrane potential in NPC cell lines. Red, JC-1 aggregates; green, JC-1 monomers; merge, combined red and green. Scale bar in the figure is 40 µm. (L–O) Apoptosis rates determined by flow cytometry. ACE knockdown decreased apoptosis induced by radiotherapy, although ACE knockdown alone did not significantly induce apoptosis. All data are presented as mean ± SEM (n = 3). N.S., p ≥ 0.05; *, p < 0.05; **, p < 0.01; ***, p < 0.001.