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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5189, RRID:AB_2837675.
展开/折叠
Amyloid precursor protein secretase; APP secretase; APPS; CATB_HUMAN; Cathepsin B heavy chain; Cathepsin B1; CathepsinB; CPSB; CTSB; cysteine protease; OTTHUMP00000116009; OTTHUMP00000229510; OTTHUMP00000229511; OTTHUMP00000229512; OTTHUMP00000229514; OTTHUMP00000229515; OTTHUMP00000229516; Preprocathepsin B;
抗原和靶标
Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum.
- P07858 CATB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MWQLWASLCCLLVLANARSRPSFHPLSDELVNYVNKRNTTWQAGHNFYNVDMSYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQYWEKI
翻译修饰 - P07858 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y33 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| Y173 | Phosphorylation | Uniprot | |
| N192 | N-Glycosylation | Uniprot | |
| C211 | S-Nitrosylation | Uniprot | |
| Y215 | Phosphorylation | Uniprot | |
| Y219 | Phosphorylation | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| Y244 | Phosphorylation | Uniprot | |
| C319 | S-Nitrosylation | Uniprot |
研究背景
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis.
Lysosome. Melanosome. Secreted>Extracellular space. Apical cell membrane>Peripheral membrane protein>Extracellular side.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Localizes to the lumen of thyroid follicles and to the apical membrane of thyroid epithelial cells (By similarity).
Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum.
Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Directly interacts with SHKBP1.
Belongs to the peptidase C1 family.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Renin secretion.
文献引用
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