Hsc70 Antibody - #AF5187

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产品: Hsc70 抗体
货号: AF5187
描述: Rabbit polyclonal antibody to Hsc70
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat, Monkey
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: P11142
RRID: AB_2837673

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
Hsc70 Antibody detects endogenous levels of total Hsc70.
RRID:
AB_2837673
引用格式: Affinity Biosciences Cat# AF5187, RRID:AB_2837673.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

2410008N15Rik; Constitutive heat shock protein 70; Epididymis luminal protein 33; Epididymis secretory sperm binding protein Li 72p; Heat shock 70 kDa protein 8; Heat shock 70kD protein 10; Heat shock 70kD protein 8; Heat shock 70kDa protein 8; Heat shock cognate 71 kDa protein; Heat shock cognate protein 54; Heat shock cognate protein 71 kDa; Heat shock protein 8; Heat shock protein A8; Heat-shock70-KD protein 10, formerly; HEL 33; HEL S 72p; HSC54; HSC71; Hsc73; HSP71; HSP73; HSP7C_HUMAN; HSPA10; HSPA8; LAP1; Lipopolysaccharide associated protein 1; LPS associated protein 1; LPS associated protein; MGC102007; MGC106514; MGC114311; MGC118485; MGC131511; MGC29929; N-myristoyltransferase inhibitor protein 71; NIP71;

抗原和靶标

免疫原:

A synthesized peptide derived from human Hsc70, corresponding to a region within the internal amino acids.

基因/基因ID:
HSPA8(3312)
描述:
Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Genetic Information Processing > Transcription > Spliceosome.

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

文献引用

1). Synergistic stimulation of osteoblast differentiation of rat mesenchymal stem cells by leptin and 25(OH)D3 is mediated by inhibition of chaperone-mediated autophagy. Stem Cell Research & Therapy, 2021 (PubMed: 34717752) [IF=7.5]

Application: WB    Species: Rat    Sample: rBMMSCs

Fig. 3 Leptin inhibited CMA of rBMMSCs by activating PI3K/AKT signal pathway. a Relative mRNA expression of CMA-related genes (LAMP2A and HSC70) of rBMMSCs in growth medium at 8 h; b western blot bands of proteins of LAMP2A and HSC70 of rBMMSCs in growth medium at 24 h; c relative quantitative analysis of proteins of LAMP2A and HSC70; d 25(OH)D3 with or without leptin did not affect the relative mRNA expression of LAMP2A and HSC70 in ODM at 8 h; e 25(OH)D3 did not affect the expression of proteins of LAMP2A and HSC70 in ODM with or without leptin at 24 h; f relative quantitative analysis of proteins of LAMP2A and HSC70; g western blot analysis of JAK2/STAT3, PI3K/AKT, and MAPK/p38 in ODM at 1 h; h relative quantitative analysis of related signal pathway proteins. (*P 
2). Aging as a Precipitating Factor in Chronic Restraint Stress-Induced Tau Aggregation Pathology, and the Protective Effects of Rosmarinic Acid. JOURNAL OF ALZHEIMERS DISEASE, 2016 (PubMed: 26577520) [IF=3.4]

Application: WB    Species: mouse    Sample: mouse

Fig. 5. The effect of chronic restraint stress on the expression of chaperone proteins. Homogenates of cerebral cortex from adult (6 months) and middle-age (13 months) mice with or without chronic restraint stress were detected by immunoblotting with antibodies against chaperones including heat shock proteins (HSP70, HSC70, HSP90, and HDJ2/HSP40) and chaperonin TCP1, and also the antibody against Pin1 (A). The protein levels were present as the fold change compared with the level in control of 13-month-old mice. The quantified data of (A) are shown in histograms (B-G). Data were expressed as mean ± SEM, n = 4. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. H) The expression of HDJ2/HSP40 or Pin1 in HEK293 cells was inhibited by siRNA, and tau gene was transfected by 2.5 g (+) or 5g (++) GV143-tau plasmids. The total extraneous tau, which contained a FLAG tag, were analyzed by anti-FLAG antibody, and P-tau was detected by PS396 antibody.
3). Memantine Differentially Regulates Tau Phosphorylation Induced by Chronic Restraint Stress of Varying Duration in Mice. NEURAL PLASTICITY, 2019 (PubMed: 30906318) [IF=3.0]

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