PAI 1 Antibody - #AF5176

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产品: PAI 1 抗体
货号: AF5176
描述: Rabbit polyclonal antibody to PAI 1
应用: WB IHC IF/ICC
反应: Human
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 46 kDa; 45kD(Calculated).
蛋白号: P05121
RRID: AB_2837662

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MS Report
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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(82%), Bovine(82%), Horse(82%), Sheep(82%), Rabbit(82%), Dog(82%)
克隆:
Polyclonal
特异性:
PAI 1 Antibody detects endogenous levels of total PAI 1.
RRID:
AB_2837662
引用格式: Affinity Biosciences Cat# AF5176, RRID:AB_2837662.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Clade E; Endothelial plasminogen activator inhibitor; Nexin; PAI 1; PAI; PAI-1; PAI1_HUMAN; PLANH1; Plasminogen activator inhibitor 1; Plasminogen activator inhibitor type 1; Serine (or cysteine) proteinase inhibitor; Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1; Serpin E1; Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1; Serpin peptidase inhibitor clade E; Serpine 1; SERPINE1;

抗原和靶标

免疫原:
Uniprot:

P05121(PAI1_HUMAN) >>Visit HPA database.

基因/基因ID:
SERPINE1(5054)
表达:
P05121 PAI1_HUMAN:

Expressed in endothelial cells (PubMed:2430793, PubMed:3097076). Found in plasma, platelets, and hepatoma and fibrosarcoma cells.

描述:
Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.
序列:
MQMSPALTCLVLGLALVFGEGSAVHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPKFSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASSSTAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
82
Horse
82
Bovine
82
Sheep
82
Dog
82
Rabbit
82
Xenopus
73
Zebrafish
45
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P05121 作为底物

Site PTM Type Enzyme
Y102 Phosphorylation
Y193 Phosphorylation
T200 Phosphorylation

研究背景

功能:

Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence. Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis.

翻译修饰:

Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in endothelial cells. Found in plasma, platelets, and hepatoma and fibrosarcoma cells.

亚基结构:

Forms a heterodimer with TMPRSS7. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Interacts with PPP1CB. In complex with PLAU/uPA, interacts with PLAUR/uPAR. Interacts with SORL1 and LRP1, either alone or in complex with PLAU; these interactions are abolished in the presence of LRPAP1/RAP. The ternary complex composed of PLAUR-PLAU-PAI1 also interacts with SORL1. Also interacts with SORL1, when complexed to PLAT/tPA.

蛋白家族:

Belongs to the serpin family.

研究领域

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

文献引用

1). Effects of (Pro) renin Receptor on Diabetic Cardiomyopathy Pathological Processes in Rats via the PRR-AMPK-YAP Pathway. Frontiers in Physiology (PubMed: 34122131) [IF=4.0]

Application: IHC    Species: Human    Sample: mesangial cells

FIGURE 2 PRR silencing decreased myocardial interstitial fibrosis and inflammation in rats with diabetic cardiomyopathy (n = 5). (A–F) Representative immunohistochemical staining of Collagen I, Fibronectin, PAI-1, TGF-β, IL-1β and IL-18 in the 4 groups (scale bar = 20 μm). (G–L) Quantitative analysis of the Collagen I, Fibronectin, PAI-1, TGF-β, IL-1β, and IL-18 protein expression shown in panels (A–F). *p < 0.05 versus the control group; #p < 0.05 versus the Ad-SC-shRNA group.
2). Yes-Associated Protein is Involved in Myocardial Fibrosis in Rats with Diabetic Cardiomyopathy. Diabetes, Metabolic Syndrome and Obesity (PubMed: 34012279) [IF=3.3]

Application: IHC    Species: Rat    Sample: myocardium

Figure 2 HE staining, CTGF, Profilin-1, and PAI-1 protein expression in the myocardium in vivo (n=6). (A-D) Representative HE staining and immunohistochemical staining of CTGF, Profilin-1 and PAI-1 in the 4 groups (scale bar=20 μm). (E-H) Quantitative analysis of CTGF, Profilin-1, and PAI-1 protein expression.
3). TGF-β1-Mediated Activation of SERPINE1 is Involved in Hemin-Induced Apoptotic and Inflammatory Injury in HT22 Cells. Neuropsychiatric Disease and Treatment (PubMed: 33603380) [IF=3.2]

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